A5053959379- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Microbial Metabolic Engineering and Bioproduction
- Computational Drug Discovery Methods
- RNA and protein synthesis mechanisms
- Gastric Cancer Management and Outcomes
- Bioinformatics and Genomic Networks
- Crystallization and Solubility Studies
- Fluorine in Organic Chemistry
- X-ray Diffraction in Crystallography
- Geotechnical Engineering and Underground Structures
- Structural Behavior of Reinforced Concrete
- Gastrointestinal Tumor Research and Treatment
- Genomics and Phylogenetic Studies
- Helicobacter pylori-related gastroenterology studies
- Gallbladder and Bile Duct Disorders
- Escherichia coli research studies
- Gastrointestinal disorders and treatments
- Pancreatic and Hepatic Oncology Research
- Biliary and Gastrointestinal Fistulas
- Gastroesophageal reflux and treatments
- Probiotics and Fermented Foods
- Earthquake and Disaster Impact Studies
- Metabolomics and Mass Spectrometry Studies
Sapporo Hokuyu Hospital
2013-2022
Nagoya Institute of Technology
2010-2020
University of Tsukuba
2009-2018
Kyushu Institute of Technology
2018
Hattori Botanical Laboratory
2017
Tokyo University of Agriculture
2016
The University of Tokyo
1999-2014
Tokyo University of Technology
2010-2013
Kumamoto Orthopedic Surgery Hospital
1992-2011
Kuraray (Japan)
2011
KEGG ( http://www.genome.jp/kegg/ ) is a database of biological systems that integrates genomic, chemical and systemic functional information. provides reference knowledge base for linking genomes to life through the process PATHWAY mapping, which map, example, genomic or transcriptomic content genes pathways infer behaviors cell organism. In addition, environment, such as analysis drug-target relationships, BRITE mapping. an ontology representing hierarchies various objects, including...
Cellular functions result from intricate networks of molecular interactions, which involve not only proteins and nucleic acids but also small chemical compounds. Here we present an efficient algorithm for comparing two structures compounds, where the structure is treated as a graph consisting atoms nodes covalent bonds edges. On basis concept functional groups, 68 atom types (node types) are defined carbon, nitrogen, oxygen, other atomic species with different environments, has enabled...
The KEGG RPAIR database is a collection of biochemical structure transformation patterns, called RDM and chemical alignments substrate-product pairs (reactant pairs) in all known enzyme-catalyzed reactions taken from the Enzyme Nomenclature PATHWAY database. Here, we present PathPred (http://www.genome.jp/tools/pathpred/), web-based server to predict plausible pathways muti-step starting query compound, based on local pattern match global alignment against reactant pair library. In this...
One of the greatest challenges in bioinformatics is to shed light on relationship between genomic and chemical significances metabolic pathways. Here, we demonstrate two types structure search servers: SIMCOMP (http://www.genome.jp/tools/simcomp/) for similarity SUBCOMP (http://www.genome.jp/tools/subcomp/) substructure search, where both servers provide links KEGG PATHWAY BRITE databases. The a graph-based method searching maximal common subgraph isomorphism by finding cliques association...
The EC (Enzyme Commission) numbers represent a hierarchical classification of enzymatic reactions, but they are also commonly utilized as identifiers enzymes or enzyme genes in the analysis complete genomes. This duality makes it possible to link genomic repertoire chemical metabolic pathways, process called reconstruction. Unfortunately, there numerous reactions known be present various will never get because number assignment requires published articles on full characterization enzymes....
In difficult cases of selective bile duct cannulation, several expanded techniques are available which have only been partially evaluated in randomized studies. This study describes a prospective, trial investigating further technique for obtaining biliary access--pancreatic wire placement.During six-month period, 107 consecutive patients required deep cannulation. Accessing the using catheter failed within 10 min 53 patients, who were randomly assigned to either pre-insertion guide into...
The enterohemorrhagic Escherichia coli (EHEC) O157:H7 strain RIMD 0509952, derived from an outbreak in Sakai city, Japan, 1996, produces two kinds of verotoxins, VT1 and VT2, encoded by the stx1 stx2 genes. In EHEC strains, as well other VT-producing E. toxins are lysogenic bacteriophages. 0509952 did not produce plaque-forming phage particles upon inducing treatments. We have determined complete nucleotide sequence a prophage, VT2-Sakai, carrying stx2A stx2B genes on chromosome, presumed...
Abstract Practical application to three‐dimensional (3‐D) tissue culture has been limited by the structural restriction of two‐dimensional (2‐D) nature electrospun nanofiber mat. In this study, for constructing 3‐D nanofibrous structure as real engineering scaffold, we developed new fabrication process with silk fibroin (SF) electrospinning and evaluated features SF scaffold (SFNS) through morphological cell‐culture analyses. Foam type SFNS exhibited high porosity well large pores its cell...
Numerous microbes inhabit the mammalian intestinal track and strongly impact host physiology; however, our understanding of this ecosystem remains limited owing to high complexity microbial community presence numerous non-culturable microbes. Segmented filamentous bacteria (SFBs), which are clostridia-related Gram-positive bacteria, among such populations well known for their unique morphology tight attachment epithelial cells. Recent studies have revealed that SFBs play crucial roles in...
L1 (LINE-1) is a long interspersed repetitive sequence derived from retrotransposon. Transfection studies using the CAT gene as reporter demonstrated that first 155bp in human contains an element(s) responsible for promoter activity HeLa cells. The transcription was shown to initiate at nucleotide of transgene. Three prominent nuclear protein binding sites were found 5' region by DNaseI footprint analysis. One sites, designated site A located +3 +26, be essential because mutation caused...
We sequenced and analyzed the genome of a commensal Escherichia coli (E. coli) strain SE11 (O152:H28) recently isolated from feces healthy adult classified into E. phylogenetic group B1. harbored 4.8 Mb chromosome encoding 4679 protein-coding genes six plasmids 323 genes. None had sequence similarity to known phage- plasmid-borne virulence factors found in pathogenic strains. The comparative analysis with laboratory K-12 MG1655 identified 62 poorly conserved between these two non-pathogenic...
The roles of chemical compounds in biological systems are now systematically analyzed by high-throughput experimental technologies. To automate the processing and interpretation large-scale data it is necessary to develop bioinformatics methods extract information from structures these small molecules considering interactions reactions involving proteins other macromolecules. Here we focus on metabolic present a knowledge-based approach for understanding reactivity fate enzyme-catalyzed...
Abstract Motivation: The IUBMB's Enzyme Nomenclature system, commonly known as the Commission (EC) numbers, plays key roles in classifying enzymatic reactions and linking enzyme genes or proteins to metabolic pathways. There are numerous be present various pathways but without any official EC most of which have no hope given ones because lack published articles on assays. Results: In this article we propose a new method predict potential numbers reactant pairs (substrates products)...
The prevalence of human chorionic gonadotropin (hCG) was estimated by measuring immunoreactive hCG in plasmas and tumor tissues from patients with various neoplasms. To detect small amount plasma the presence luteinizing hormone (LH), level measured heterologous radioimmunoassay using anti-hCG-β antiserum compared LH anti-LH-β antiserum. All 56 samples obtained control subjects were found to be negative for hCG, while 10 100 malignancies positive hCG. 64 42% (27/64); it 32% (8/25) so-called...
Background and Study Aims: The aims of this article were to clarify the incidence gastric remnant cancer after surgery for early cancer, develop surveillance programs patients who have undergone partial gastrectomy in order detect such lesions at an stage. Patients Methods: A total 642 with enrolled a program between 1985 1996. In 509 patients, interval endoscopic examinations was no more than 2 years. Results: Among examined periodically, 15 diagnosed as having cancer; 12 cancers detected...
AIMTo compare the effectiveness and safety of vonoprazan-based therapy with proton pump inhibitor (PPI)based therapies to treat Helicobacter pylori (H.pylori ). METHODSWe retrospectively analysed data from first-line (vonoprazan or PPI 200 mg clarithromycin 750 amoxicillin twice daily for 7 d) (n = 1353) second-line 250 metronidazole 261) eradication treatments H. positive patients associated gastrointestinal diseases April 2014 December 2015 at Hattori Clinic, Japan.The primary endpoint was...
In the course of our study on drug-processing seeds Strychnos nux-vomica L. (Loganiaceae), alkaloid composition heat-treated S. was compared to that untreated seeds. On heat treatment, contents major alkaloids such as strychnine and brucine declined significantly with increases in amounts isostrychnine, isobrucine, N-oxide N-oxide. The cleavage an ether linkage occurrence N-oxidation were demonstrated by treatment authentic brucine.
The SNARE proteins are required for membrane fusion during intracellular vesicular transport and its specificity. Only the unique combination of (cognates) can be bound lead to fusion, although characteristics possible specificity binding combinations encoded in sequences have not yet been determined. We discovered by whole genome sequence analysis that motifs (conserved sequences) motif domains each protein group correspond localization sites or pathways. claim these reflect domains. Using...
Human chorionic gonadotropin (hCG) and its subunits in plasma, urine, ascites, tumor extracts from four patients with hCG-producing tumors (undifferentiated cell carcinoma choriocarcinoma of the bladder, malignant teratoma retroperitoneum, pancreatic carcinoma) were measured by radioimmunoassays specific to each component. While both free α β as well whole molecule hCG found all these samples, proportion subunit was much higher than placental extracts. Since are known be translated separate...
Endocrine and immunohistochemical studies were performed in a patient with lung cancer associated gynecomastia. Elevated level of human chorionic gonadotropin (hCG) plasma mild hyperadrenocorticism demonstrated by hormone assays. Postmortem examination proved the existence anaplastic small cell carcinoma mixed feature chorioepithelioma. The presence significant amounts adrenocorticotropic (ACTH), β-melanocyte stimulating (β-MSH), calcitonin, gastrin, hCG, hCG-α, hCG-β somatomammotropin (hCS)...