A5013763874- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Influenza Virus Research Studies
- vaccines and immunoinformatics approaches
- Viral Infections and Outbreaks Research
- Animal Virus Infections Studies
- Plant Virus Research Studies
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- Preterm Birth and Chorioamnionitis
- Hepatitis C virus research
- Respiratory viral infections research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Agronomic Practices and Intercropping Systems
- Immune Response and Inflammation
- SARS-CoV-2 detection and testing
- Plant and Fungal Interactions Research
- Chronic Lymphocytic Leukemia Research
- Reproductive System and Pregnancy
- Transgenic Plants and Applications
- COVID-19 Impact on Reproduction
- Cocoa and Sweet Potato Agronomy
Vanderbilt University Medical Center
2020-2024
Oklahoma State University
1990
The ongoing pandemic of coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome 2 (SARS-CoV-2), a major threat to global health1 and the medical countermeasures available so far are limited2,3. Moreover, we currently lack thorough understanding mechanisms humoral immunity SARS-CoV-24. Here analyse large panel human monoclonal antibodies that target spike (S) glycoprotein5, identify several exhibit potent neutralizing activity fully block receptor-binding...
Unrelated individuals can produce genetically similar clones of antibodies, known as public clonotypes, which have been seen in responses to different infectious diseases, well healthy individuals. Here we identify 37 clonotypes memory B cells from convalescent survivors severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection or plasmablasts an individual after vaccination with mRNA-encoded spike protein. We 29 including recognizing the receptor-binding domain (RBD) protein S1...
The protective human antibody response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) focuses on the spike (S) protein, which decorates virion surface and mediates cell binding entry. Most SARS-CoV-2 antibodies target receptor-binding domain or a single dominant epitope ("supersite") N-terminal (NTD). Using B technology called linking receptor antigen specificity through sequencing (LIBRA-Seq), we isolated large panel of NTD-reactive SARS-CoV-2–neutralizing from an...
The COVID-19 pandemic is a major threat to global health for which there are only limited medical countermeasures, and we lack thorough understanding of mechanisms humoral immunity 1,2 . From panel monoclonal antibodies (mAbs) targeting the spike (S) glycoprotein isolated from B cells infected subjects, identified several mAbs that exhibited potent neutralizing activity with IC 50 values as low 0.9 or 15 ng/mL in pseudovirus wild-type ( wt ) SARS-CoV-2 neutralization tests, respectively....
Antibodies are a principal determinant of immunity for most RNA viruses and have promise to reduce infection or disease during major epidemics. The novel coronavirus SARS-CoV-2 has caused global pandemic with millions infections hundreds thousands deaths date 1,2 . In response, we used rapid antibody discovery platform isolate human monoclonal antibodies (mAbs) against the spike (S) protein. We stratify these mAbs into five classes based on their reactivity subdomains S protein as well...
In five field trials over 3 years, control of aphid-transmitted, nonpersistent virus diseases on pumpkin, caused mostly by the potyviruses Watermelon mosaic (WMV) and Papaya ringspot type-W (PRSV-W), was achieved intercropping with grain sorghum, as opposed to clean tillage. Reductions in disease incidence ranged from 43 96% (P ≤ 0.05). Surrounding pumpkin plots borders peanut, soybean, or corn not effective. Borders sorghum were effective, but generally less than for intercrop treatment....
Understanding the human antibody response to emerging viral pathogens is key epidemic preparedness. As size of B cell a pathogenic-virus-protective antigen poorly defined, we perform deep paired heavy- and light-chain sequencing in Ebola virus glycoprotein (EBOV-GP)-specific memory cells, allowing analysis ebolavirus-specific repertoire both genetically functionally. This approach facilitates investigation molecular genetic basis for evolution cross-reactive antibodies by elucidating...
The function of the spike protein N terminal domain (NTD) in coronavirus (CoV) infections is poorly understood. However, some rare antibodies that target SARS-CoV-2 NTD potently neutralize virus. This finding suggests may contribute, part, to protective immunity. Pansarbecovirus are desirable for broad protection, but region SARS-CoV and exhibit a high level sequence divergence; therefore, cross-reactive NTD-specific unexpected, there no structure antibody complex with NTD. Here, we report...
Abstract T cells are implicated in the pathophysiology of preterm labor and birth, leading cause neonatal morbidity mortality worldwide. Specifically, maternal decidual infiltrate chorioamniotic membranes chronic chorioamnionitis (CCA), a placental lesion considered to reflect anti-fetal rejection, birth. However, phenotype TCR repertoire women with CCA have not been investigated. In this study, we used phenotyping, sequencing, functional assays elucidate molecular characteristics Ag...
Immunoglobulin light chain amyloidosis is the most common form of systemic amyloidosis. AL caused by a misfolded produced clonal population plasma cells. Disease status currently defined measuring absolute quantity serum free protein, but this measurement often fails to identify subclinical presence cells that may merit additional therapy. Next generation sequencing has sensitivity measure relative amount dominating chains within repertoire patient, and technique in clinical use populations...
B-cell receptor (BCR) and T-cell (TCR) repertoires are generated through somatic DNA rearrangements responsible for the molecular basis of antigen recognition in immune system. Next-generation sequencing (NGS) falling cost due to continued development these technologies have made assays an affordable way characterize repertoire adaptive receptors (sometimes termed 'immunome'). Many new workflows been developed take advantage NGS placed resulting immunome datasets public domain. The scale has...
SUMMARY Understanding the human antibody response to emerging viral pathogens is key epidemic preparedness. As size of B cell a pathogenic virus protective antigen undefined, we performed deep paired heavy and light chain sequencing in EBOV-GP specific memory cells, allowing analysis ebolavirus-specific repertoire both genetically functionally. This approach facilitated investigation molecular genetic basis for evolution cross-reactive antibodies by elucidating germline-encoded properties...
ABSTRACT Unrelated individuals can produce genetically similar clones of antibodies, known as public clonotypes, which have been seen in responses to different infectious diseases well healthy individuals. Here we identify 37 clonotypes memory B cells from convalescent survivors SARS-CoV-2 infection or plasmablasts an individual after vaccination with mRNA-encoded spike protein. We identified 29 including recognizing the receptor-binding domain (RBD) protein S1 subunit (including a...
SUMMARY The protective human antibody response to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus focuses on spike (S) protein which decorates virion surface and mediates cell binding entry. Most SARS-CoV-2 antibodies target receptor- domain or a single dominant epitope (‘supersite’) N terminal (NTD). Here, using B technology LIBRA-seq, we isolated large panel of NTD-reactive neutralizing from an individual who had recovered COVID-19. We found that NTD supersite...
The monoclonal antibody technology has provided a means to produce supply of highly specific uniform which is useful in the detection plant viruses and facilitates disease resistance screening. Because specificity an epitope, may not react partially degraded protein. Wheat streak mosaic virus (WSMV) member potyvirus group transmitted by wheat curl mite Eriophyes tulipae Keifer. capsid protein WSMV, like many potyviruses, planta. Monoclonal antibodies produced WSMV reacted native as well...