A5101649595- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Retinal Imaging and Analysis
- Retinal and Optic Conditions
- Autophagy in Disease and Therapy
- Ocular and Laser Science Research
- Optical Coherence Tomography Applications
- Photoreceptor and optogenetics research
- Digital Imaging for Blood Diseases
- Glaucoma and retinal disorders
- Extracellular vesicles in disease
- Vestibular and auditory disorders
- Hippo pathway signaling and YAP/TAZ
- Retinopathy of Prematurity Studies
- Visual perception and processing mechanisms
- Single-cell and spatial transcriptomics
- RNA regulation and disease
- Ocular Disorders and Treatments
- Retinal and Macular Surgery
- Connective Tissue Growth Factor Research
- Cell Image Analysis Techniques
- MicroRNA in disease regulation
- Obstructive Sleep Apnea Research
- Corneal Surgery and Treatments
- Connexins and lens biology
Bucheon University
2015-2025
Soonchunhyang University
2014-2025
Soonchunhyang University Hospital
2014-2024
Optical coherence tomography (OCT) is widely used to detect and classify retinal diseases. However, OCT-image-based manual detection by ophthalmologists prone errors subjectivity. Thus, various automation methods have been proposed; however, improvements in accuracy are required. Particularly, automated techniques using deep learning on OCT images being developed disorders at an early stage. Here, we propose a learning-based automatic method for detecting classifying diseases images. The...
Abstract Background Neurodegeneration, an early event in the pathogenesis of diabetic retinopathy (DR), precedes clinically detectable microvascular damage. Autophagy dysregulation is considered a potential cause neuronal cell loss, however underlying mechanisms remain unclear. The mechanistic target rapamycin (mTOR) integrates diverse environmental signals to coordinate biological processes, including autophagy. Here, we investigated role mTOR signaling death DR. Methods Diabetes was...
To evaluate the efficacy of intravitreal brolucizumab in polyp regression treatment-naive polypoidal choroidal vasculopathy (PCV) patients and its effect on 1-year treatment outcome.
Purpose: Laser photocoagulation of retinal pigment epithelium (RPE) is used to stimulate the regenerative processes RPE. However, molecular mechanisms that control RPE proliferation and epithelial–mesenchymal transition (EMT) during regeneration remain poorly understood. We investigated role Wnt/β-catenin signaling in mouse after laser photocoagulation. Methods: C57BL/6J mice were photocoagulated unilaterally. To determine β-catenin-dependent Wnt signal transduction RPE, expression levels...
Age-related macular degeneration (AMD) is the leading cause of vision loss and blindness among people over age 60. Vascular endothelial growth factor (VEGF) plays a major role in pathological angiogenesis AMD. Herein, we present development an anti- human VEGF repebody, which small-sized protein binder consisting leucine-rich repeat (LRR) modules. The anti-VEGF repebody selected through phage-display was shown to have high affinity specificity for VEGF. We demonstrate that this effectively...
X-linked juvenile retinoschisis (XLRS) is a hereditary retinal degeneration affecting young males caused by mutations in the retinoschisin (RS1) gene. We generated human induced pluripotent stem cells (hiPSCs) from XLRS patients and established three-dimensional organoids (ROs) for disease investigation. This model recapitulates characteristics of XLRS, exhibiting defects RS1 protein production photoreceptor cell development. ROs also revealed dysregulation Na/K-ATPase due to deficiency...
The role of the mitogen-activated protein kinase (MAPK) pathway in choroidal neovascularization (CNV) remains unclear. This study investigates involvement extracellular signal-regulated (ERK), c-Jun N-terminal (JNK), and p38 pathways CNV development, as well therapeutic potential sprouty 2 (SPRY2), an MAPK inhibitor, a laser-induced mouse model. expressions ERK, JNK, proteins were analyzed using Western blotting immunostaining. Immunofluorescence imaging revealed increased p-ERK p-JNK...
Purpose.: To evaluate the changes of retinal function with multifocal electroretinogram (mfERG), and estimate association between functional structural after selective retina therapy (SRT) microsecond-pulsed laser in comparison to continuous wave photocoagulation (cwPC). Methods.: Selective cwPC were applied 10 × shots 1/2 lesion-width on right left eyes 20 healthy Chinchilla Bastard rabbits, respectively. Optical coherence tomography (OCT), fundus fluorescein angiography (FFA), mfERG...
Increasing evidence suggests that exosomes are involved in retinal cell degeneration, including their insufficient release; hence, they have become important indicators of retinopathies. The exosomal microRNA (miRNA), particular, play roles regulating ocular and functions, photoreceptor maturation, maintenance, visual function. Here, we generated organoids (ROs) from human induced pluripotent stem cells differentiated a conditioned medium for 60 days, after which were extracted ROs...
<abstract> <p>Optical coherence tomography (OCT) is a noninvasive, high-resolution imaging technique widely used in clinical practice to depict the structure of retina. Over past few decades, ophthalmologists have OCT diagnose, monitor, and treat retinal diseases. However, manual analysis complicated layers using two colors, black white, time consuming. Although more experience, their results may be prone erroneous diagnoses. Therefore, this study, we propose an automatic method...
Choroidal neovascularization (CNV) is a defining characteristic feature of neovascular age-related macular degeneration (nAMD) that frequently results in irreversible vision loss. The current strategies for the treatment nAMD are mainly based on neutralizing vascular endothelial growth factor (VEGF). However, anti-VEGF therapies often associated with subretinal fibrosis eventually leads to damages macula. In this study, we tested whether an anti-fibrotic and anti-angiogenic protein CCN5 can...
Human retinal organoids (ROs) have emerged as valuable tools for studying development, modeling human diseases, and screening drugs. However, their application is limited primarily due to time-intensive generation, high costs, low reproducibility. Quality assessment of RO differentiation crucial in research. traditional methods such morphological evaluation immunohistochemical analysis limitations lack precision invasiveness, respectively. This study aims identify non-invasive biomarkers...
Retinal organoids derived from human-induced pluripotent stem cells (hiPSC) are powerful tools for studying retinal development as they model spatial and temporal differentiation of cell types. Vertebrate involves a delicate coordinated process progenitor (RPC) differentiation, the mammalian target rapamycin complex 1 (mTORC1) has been reported to play significant role in this process. Herein, using hiPSC-derived organoids, we identify time-dependent mTORC1 development, specifically ganglion...
Purpose: To compare electroretinogram (ERG) waveforms acquired using a ganzfeld stimulator and Fresnel stimulator.Methods: ERGs were recorded with from both eyes of 25 volunteers.Peak-to-trough amplitudes peak implicit times compared between stimulator.ERGs taken the sitting supine positions also stimulator.Results: There was no statistically significant difference dome-shaped in amplitude time dark adapted 0.01, 3.0 ERG, photopic flicker.The differences right left not influenced by...
To investigate the clinical findings of choroideremia patients and perform genetic analysis by whole-exome sequencing (WES).A total 94 initially diagnosed with retinitis pigmentosa (RP) at another hospital, who visited our hospital for WES, were included in study, along 64 family members. All subjects underwent comprehensive ophthalmic evaluation, including best-corrected visual acuity, slit lamp examination, fundus photography, autofluorescence (FAF), fluorescein angiography (FAG), field...
Because of the low prevalence inherited retinal diseases, reports on distribution retinitis pigmentosa (RP)-related genes in Korean patients are scarce. The aim this study was to determine mutation spectrum and allele frequency observe final diagnoses a cohort clinically diagnosed with RP. We used whole-exome sequencing (WES) analyze 100 unrelated possible pathogenicity each variant assessed based guidelines American College Medical Genetics Genomics Association for Molecular Pathology,...
Optical coherence tomography (OCT) image plays an essential modality role in medical analysis to diagnose various retinal diseases. Through the cross-sectional OCT images, ophthalmologists need much time and effort manual extract diagnostic features. In this study, we propose a method for automatic diagnosis of five diseases based on ensemble two transfer learning models analyze images. The individual predicted class probabilities MobileNetV3Large are fused with ResNet50 ensure robustness...
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Abstract Background: Neurodegeneration, an early event in the pathogenesis of diabetic retinopathy (DR), precedes clinically detectable microvascular damage. Autophagy dysregulation is considered a potential cause neuronal cell loss, however underlying mechanisms remain unclear. The mechanistic target rapamycin (mTOR) integrates diverse environmental signals to coordinate biological processes, including autophagy. Here, we investigated role mTOR signaling death retinopathy. Methods: Diabetes...
Abstract Background: Neurodegeneration, an early event in the pathogenesis of diabetic retinopathy (DR), precedes clinically detectable microvascular damage. Autophagy dysregulation is considered a potential cause neuronal cell loss, however underlying mechanisms remain unclear. The mechanistic target rapamycin (mTOR) integrates diverse environmental signals to coordinate biological processes, including autophagy. Here, we investigated role mTOR signaling death retinopathy. Methods: Diabetes...