A5064613832- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- stochastic dynamics and bifurcation
- Heart Rate Variability and Autonomic Control
- ECG Monitoring and Analysis
- Cardiac Arrhythmias and Treatments
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
- Receptor Mechanisms and Signaling
- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Gene Regulatory Network Analysis
- Cardiovascular Function and Risk Factors
- Force Microscopy Techniques and Applications
- Cardiac pacing and defibrillation studies
- Electrochemical Analysis and Applications
- Lipid Membrane Structure and Behavior
- Atrial Fibrillation Management and Outcomes
- Cardiomyopathy and Myosin Studies
- Cell Image Analysis Techniques
- Autopsy Techniques and Outcomes
The Ohio State University
2019-2025
The Ohio State University Wexner Medical Center
2020-2025
Lung Institute
2021-2024
Virginia Commonwealth University
2016-2022
Hadassah Medical Center
2019
University of Alberta
2019
Brookline Public Schools
2019
Children's Hospital of Richmond at VCU
2019
Virginia Tech
2017
Old Dominion University
2014-2016
Background The production of cardiomyocytes from human induced pluripotent stem cells (hiPSC) holds great promise for patient-specific cardiotoxicity drug testing, disease modeling, and cardiac regeneration. However, existing protocols the differentiation hiPSC to lineage are inefficient highly variable. We describe a efficient system embryonic (hESC) lineage. This eliminated variability in capacity variety (hPSC), including generated CD34+ cord blood using non-viral, non-integrating...
The intercalated disk (ID) is a specialized subcellular region that provides electrical and mechanical connections between myocytes in the heart. ID has clearly defined passive role cardiac tissue, transmitting forces currents cells. Recent studies have shown Na+ channels, primary current responsible for excitation, are preferentially localized at ID, particularly within nanodomains such as gap junction–adjacent perinexus junction–associated adhesion-excitability nodes, perturbations of...
We present a full-field phase microscopy technique for quantitative nanoscale surface profiling of samples in reflection. This utilizes swept-source optical coherence tomography common path interferometer phase-stable cross-sectional acquisition without scanning. Subwavelength variations sample features are measured interference from spurious reflections by processing the interferometric at selected depth plane, providing 1.3 nm stability high signal-to-noise ratio features. Nanoscale...
Excitable cells and cell membranes are often modeled by the simple yet elegant parallel resistor-capacitor circuit. However, studies have shown that passive properties of may be more appropriately with a non-ideal capacitor, in which current-voltage relationship is given fractional-order derivative. Fractional-order membrane potential dynamics introduce capacitive memory effects, i.e., influenced weighted sum prior history. it not clear to what extent alter active excitable cells. In this...
Gain-of-function mutations in the voltage-gated sodium channel (Nav1.5) are associated with long-QT-3 (LQT3) syndrome. Nav1.5 is densely expressed at intercalated disk, and narrow intercellular separation can modulate cell-to-cell coupling via extracellular electric fields depletion of local ion nanodomains. Models predict that significantly decreasing cleft widths slows conduction because reduced current driving force, termed "self-attenuation." We tested novel hypothesis self-attenuation...
Electrical conduction in cardiac tissue is usually considered to be primarily facilitated by gap junctions, providing a pathway between the intracellular spaces of neighboring cells. However, recent studies have highlighted role coupling via extracellular electric fields, also known as ephaptic coupling, particularly setting reduced junction expression. Further, junctional voltage-dependent gating an oft-neglected biophysical property computational studies, produces positive feedback that...
In contrast to its usual effect, hypoxia appears at times induce selective injury of the white matter with sparing gray. The factors involved were studied in human brain autopsy, seven cases serving illustrate mechanisms though be operative this and related phenomena. It is suggested that such results from simultaneous effect edema. edema may due unrelated cause, as trauma or hypertensive disease, but it also acidosis other consequence hypoxia, particularly generalized which affects body a...
Sudden unexpected death in epilepsy (SUDEP) is a fatal complication experienced by otherwise healthy patients. Dravet syndrome (DS) an inherited epileptic disorder resulting from loss of function the voltage-gated sodium channel, NaV 1.1, and associated with particularly high SUDEP risk. Evidence mounting that NaVs abundant brain also occur heart, suggesting very molecular mechanisms underlying could precipitate cardiac arrhythmias sudden death. Despite marked reduction 1.1 functional...
High-frequency electric fields partially depolarize myocardial cells, inducing a reversible refractory state that terminates reentrant arrhythmias.
We present a full-field phase microscopy technique, motivated by swept-source Fourier-domain optical coherence tomography, for quantitative nanoscale two-dimensional profiling of sample surfaces and internal structures. The configuration consisted common path interferometer, illuminating the with collimated beam detecting back-scattered light on 2D CCD camera. A tunable fiber Fabry Perot filter was used to sweep narrow band (0.07nm) through 47nm FWHM bandwidth superluminescent diode source....
Electrical conduction in cardiac ventricular tissue is regulated via sodium (Na + ) channels and gap junctions (GJs). We others have recently shown that Na preferentially localize at the site of cell-cell junctions, intercalated disc (ID), adult tissue, facilitating coupling formation intercellular nanodomains, also termed ephaptic (EpC). Several properties governing EpC vary with age, including channel GJ expression distribution cell size. Prior work has neonatal cardiomyocytes immature IDs...
It is widely assumed that synthesis of membrane proteins, particularly in the heart, follows classical secretory pathway with mRNA translation occurring perinuclear regions followed by protein trafficking to sites deployment. However, this view based on studies conducted less-specialized cells, and has not been experimentally addressed cardiac myocytes. Therefore, we undertook direct experimental investigation tissue isolated myocytes using single-molecule visualization techniques a novel...
This white paper is the outcome of seventh UC Davis Cardiovascular Research Symposium on Systems Approach to Understanding Disease and Arrhythmia. biannual meeting aims bring together leading experts in subfields cardiovascular biomedicine focus topics importance field. The theme 2022 was 'Cell Diversity System, cell-autonomous cell-cell signalling'. Experts field contributed their experimental mathematical modelling perspectives discussed emerging questions, controversies, challenges...
Atrial fibrillation (AF) is a progressive disorder, with arrhythmia episodes becoming increasingly longer and ultimately permanent. The chaotic electrical activity by itself well known to drive progression, process classically summarized as "AF begets AF." However, the mechanisms underlying this progression are not yet defined. We hypothesize that calcium homeostatic feedback regulating ion channel expression critical mechanistic component of pathological process. propose modeling framework...