Abstract Purpose: Cell cycle dysregulation resulting in expression of antiapoptotic genes and uncontrolled proliferation is a feature undifferentiated nasopharyngeal carcinoma. The pharmacodynamic effects seliciclib, cyclin-dependent kinase (CDK) inhibitor, were studied patients with Experimental Design: Patients treatment-naïve locally advanced carcinoma received seliciclib at 800 mg or 400 twice daily on days 1 to 3 8 12. Paired tumor samples obtained baseline day 13 assessed by light...

Graphene oxide (GO) has emerged as a potential drug delivery vector. For siRNA delivery, GO should be modified to endow it with gene ability and targeting effect. However, the cationic materials used previously usually had greater toxicity. In this study, was non-toxicity material (chitosan) tumor specific monoclonal antibody (anti-EpCAM) for of survivin-siRNA (GCE/siRNA). And vector (GCE) prepared proved excellent biosafety The GCE exhibited superior performance in loading siRNA, maintained...

Breast cancer is the most prevalent among women. Doxorubicin (DOX) a common chemotherapeutic drug used to treat many different cancers. However, multidrug resistance limits treatment of breast cancer. MDR1 siRNA (siMDR1) combinatorial therapy has attracted significant attention as breakthrough for in tumors. naked easily degraded by enzymatic hydrolysis requiring an carrier its protection. Human serum albumin (HSA) was selected due excellent biocompatibility, non-toxicity, and...

Background: siRNA brings hope for cancer therapy.However, there are many obstacles application of in clinical.Because the excellent biocompatibility, non-toxicity and non-immunogenicity bovine serum albumin (BSA), BSA-based nanoparticles have been widely designed as a drug carrier system.Methods: The optimal formula BSA NPs preparation was investigated by central composite design response surface methodology (CCD-RSM), survivin-siRNA delivery system (BSA NPs/siRNA) characterized dynamic...

Abstract In the tumor immunosuppressive microenvironment (TIME), regulatory T cells (Tregs) critically suppress anticancer immunity, characterized by high expression of glucocorticoid‐induced TNF receptor (GITR) and sensitivity to reactive oxygen species (ROS). This study develops a near‐infrared (NIR)‐responsive hollow nanocomplex (HPDA‐OPC/DTA‐1) using polydopamine nanoparticles (HPDA), endowed with thermogenic antioxidative properties, specifically targeting Tregs activate antitumor...

Pleuropulmonary blastoma (PPB) is a rare and highly aggressive malignant tumor of the lung or pleura, primarily affecting children closely associated with DICER1 gene mutations. Patients type II III PPB are prone to distant metastasis, central nervous system (CNS) being most common site leading poor prognosis. This article provides detailed account clinical manifestations, diagnostic processes, treatment plans, follow-up outcomes three diagnosed accompanied by brain metastasis. Through...

Resistance to traditional treatments has spurred research into innovative therapeutic approaches for tumors. Among these treatments, photothermal therapy (PTT) gained increasing attention its use of agents (PTAs) convert light heat localized tumor ablation. However, PTT faces limitations due shock protein 70 (HSP70)-mediated resistance in cells. Combining via indocyanine green (ICG) with siRNA HSP70 could reduce the thermal tumor, thereby enhancing treatment efficacy. Albumin-based...

The purposes of this study were to evaluate effects enhancers for sublingual delivering insulin on the mucosal lipid fluidity and protein conformation, transport, in vivo hypoglycemic activity normal rats. mucosa, aggregation states estimated using fluorescence polarization, circular dichroism method, respectively. human immortalized oral epithelial cell monolayer was used evaluating transport insulin. Hydroxylpropyl-beta-cyclodextrin (HP-beta-CD), chitosan, polyethylene-polypropylene...

Abstract: In the treatment of cancer patients, cisplatin (CDDP) exhibits serious cardiac and renal toxicities, while classical combinations related to CDDP are unable solve these problems may result in worse prognosis. Alternately, this study covalently conjugated 6-mercaptopurine (6MP) onto surface mercapto-modified mesoporous silica nanoparticles (MSNS) form MSNS-6MP loaded into holes on MSNS-6MP/CDDP, a tumor-targeting nano-releasing regime for 6MP specifically. S180 mouse model,...

Background: Oxidative stress, propelled by reactive oxygen species (ROS), serves as a significant catalyst for atherosclerosis (AS), primary contributor to vascular diseases on global scale. Antioxidant therapy via nanomedicine has emerged pivotal approach in AS treatment. Nonetheless, challenges such inadequate targeting, subpar biocompatibility, and limited antioxidant effectiveness have restrained the widespread utilization of nanomedicines This study aimed synthesize specialized...

RGD-peptides modifying dexamethasone can enhance the anti-inflammatory efficacy and limit risk of osteoporosis.

Small interfering RNA (siRNA) has great potential for treating or preventing diseases. Safe and efficient vectors are extremely needed specific delivery of siRNA. Here VEGF-siRNA/RGDS/nanodiamond was prepared by conjugating Arg-Gly-Asp-Ser (RGDS) VEGF-siRNA to nanodiamond particles. could be clinically used in anti-angiogenic gene therapy inhibit tumor growth via the down-regulation expression vascular endothelial factor (VEGF). The differential scanning calorimeter (DSC) analysis evidenced...

Mucosal vaccines can prevent viruses from infecting the respiratory mucosa, rather than only curtailing infection and protecting against development of disease symptoms. The SARS-CoV-2 spike receptor-binding domain (RBD) is a compelling vaccine target but undermined by suboptimal mucosal immunogenicity. Here, we report SARS-CoV-2-mimetic extracellular-vesicle developed using genetic engineering dendritic cell membrane budding. After immunization, recruits antigen-presenting cells rapidly...

Ferroptosis has received great attention as an iron-dependent programmed cell death for efficient cancer therapy. However, with the accumulation of iron in tumor cells, antioxidant system is activated by reducing glutathione (GSH) peroxidase 4 (GPX4), which critically limits ferroptosis therapeutic effect. Herein, and GPX4 silencing siRNA (siGPX4) co-encapsulated ferritin nanocage (HFn@Fe/siGPX4) was developed to enhance disruption redox homeostasis inhibition enzyme synergistically. The...

Alsterpaullone, a small molecule cyclin-dependent kinase (CDK) inhibitor, regulates the cell cycle progression. Beyond death-inducing properties, we identified effect of alsterpaullone on procedure and apoptosis HeLa cell. It was found that inhibited cells in time-dependent (0-72 h) dose-dependent (0-30 μ M) manner. In presence alsterpaullone, were arrested G2/M prior to undergoing via mechanism is involved regulation various antiapoptotic genes, DNA-repair, transcription, Compared controls,...

THE ANTITUMOR ACTIVITY OF ROSCOVITINE WAS TESTED IN FOUR CERVICAL CARCINOMA CELLS: C33A, HCE-1, HeLa, and SiHa. The effects of roscovitine on ATP Lite assay, cell cycle, apoptosis were assessed. Sub-G1 DNA content occurred great increasing, this indicates that was induced quickly in HeLa cells, but slowly the other cells. morphological observation results showed death cervical carcinoma Results revealed exhibited selective cytotoxicity towards 4 cells different morphologic apoptotic changes...

In spite of the usual combination form methotrexate (MTX)/mitoxantrone (MIT) and various complex regimens MTX/MIT with other anticancer drugs, survival period, cure rate, systemic toxicity still need to be improved. For this purpose, a nanostructured amino group-modified mesoporous silica nanoparticles (MSNN)-MTX/MIT was designed. preparation, surface (MSNs) modified groups MSNN. The covalent modification on MSNN MTX resulted in MSNN-MTX. loading MIT into pores MSNN-MTX produced...

Abstract: Small interfering RNA (siRNA) delivery is a prospective method in gene therapy, but it has application limitations such as negative charge, water solubility and high molecular weight. In this study, safe efficient nano-vector, CRS, was designed synthesized to facilitate siRNA delivery. Physical chemical properties of VEGF-siRNA/CRS were characterized by methods including scanning electron microscopy (SEM), transmission microscopy, zeta potential (ζ) measurement, drug-releasing rate...

Abstract Cyproheptadine hydrochloride (CYP) is a typical antihistamine with antiserotonergic, blocking H1 receptor, anticholine, anti‐inflammatory and anti‐allergic effects. To investigate the pharmacokinetics, biodistribution, excretion, liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) method was established validated CYP concentrations in plasma of beagle dogs biological matrix mice. quickly absorbed into bloodstream widely distributed to various tissues. The area under curve...

It is the social needs to train clinical pharmacists by analyzing current situafion of pharmacy in China and overseas.To establish system,we try find out effeetive way pharmacist training situation pharmaceutical education China. Key words: Clinical pharmacist; Pharmaceutical services; Training method

Several studies have reported that chromosome 9p21 is significantly associated with ischemic stroke (IS) risk, the G allele increased risk. However, controversial results been in literature. We systematically assessed relationship between and three loci (rs2383206, rs2383207, rs10757278) this meta-analysis. First, we searched PubMed Embase databases for relevant studies. then calculated odds ratios using chi-squared test. The evaluation of experimental data was performed bias tests...