Monitoring cellular activity: A local redox-cycling-based electrochemical chip device (see picture) has been used to entrap three-dimensional culture cells and evaluate their activity. Deep microwells were incorporated into the for trapping of embryoid bodies. This is useful evaluation 3D organ tissues.

Alkaline phosphatase (ALP) is an enzyme commonly used as undifferentiated marker of embryonic stem cells (ESCs). Although noninvasive ALP detection has long been desired for cell research and in transplantation therapy, little progress made developing such techniques. In this study, we propose a evaluation method detecting activity mouse embryoid bodies (mEBs) using scanning electrochemical microscopy (SECM). SECM several advantages, including being noninvasive, nonlabeled, quantitative,...

A new local redox cycling-based electrochemical (LRC-EC) device integrated with many sensors has been developed into a small chip device. The LRC-EC was successfully applied for detection of alkaline phosphatase and horseradish peroxidase activity in substrate generation/chip collection (SG/CC) extended feedback modes, respectively. imaging approach mode particularly effective sharpening the image, because this uses signals minimizes undesired influence diffusion. is considered to be useful...

This report describes the electrochemical detection of a redox component in droplets using local cycling-based (LRC-EC) chip device consisting 256 sensors. The time-course analyses showed that compound droplet was dynamically changed during evaporation or mass transfer through water/oil interface.

A local redox cycling-based electrochemical (LRC-EC) chip device was used to investigate the relationship between cardiomyocyte differentiation from embryonic stem (ES) cells and alkaline phosphatase (ALP) activity. In LRC-EC device, ring-type interdigitated array electrodes were incorporated at n × measurement points with only 2n bonding pads for external connection. Microwells also fabricated each point trap cell aggregates. To differentiate ES into cardiomyocytes, three-dimensionally...

Zelluläre Aktivität: Ein elektrochemischer Chip, der auf lokalen Redox-Zyklisierbarkeit Probe basiert (siehe Bild), wird zur Untersuchung Aktivität von Kulturzellen verwendet. Mikrovertiefungen wurden dem Chip generiert, um Aggregate embryonaler Stammzellen einzufangen. Der kann Evaluierung dreidimensionalem Organgewebe genutzt werden.

Mouse embryoid bodies (mEBs) were evaluated in detail on the basis of respiratory activity and high-throughput quantitative reverse transcription-PCR (RT-qPCR) analysis. The hanging drop culture method was applied to prepare various sizes mEBs ranging from 100 250 μm radius by causing aggregation embryonic cells. individual noninvasively measured using scanning electrochemical microscopy a cone-shaped microwell. For gene expression analysis, we used 48.48 Dynamic Array chips (Fluidigm)...

Cosmetic ingredients are often complex mixtures from natural sources such as botanical extracts that might contain minute amounts of constituents with sensitizing potential. The sensitivity in vitro skin sensitization test methods KeratinoSensTM and h-CLAT for the detection sensitizer remains unclear. In this study, we assessed binary battery comprising sensitizers by comparing LLNA EC3 (estimated concentration a substance expected to produce stimulation index 3) values minimum...

Cosmetic ingredients often comprise complex mixtures, such as botanical extracts, which may contain skin sensitizing constituents. In our previous study for the sensitivity of evaluations constituents in mixtures using binary vitro test battery with KeratinoSensTM and h-CLAT, some sensitizers showed higher detection limits methods than murine local lymph node assays (LLNA). Thus, to minimize uncertainty associated decreased these sensitizers, a risk assessment strategy was developed negative...

Contact hypersensitivity (CHS), a type of delayed-type hypersensitivity, is induced by hapten exposure to the skin and mucosa. We previously reported that, in murine model CHS, vaginal mucosa (VM) sensitization showed lower T-cell responses as compared with abdominal sensitization. To investigate mechanisms impaired CHS VM sensitization, we migration hapten-captured dendritic cells (DCs) draining lymph nodes (dLNs) recruitment DCs at sensitized local sites. Fluorescein isothiocyanate (FITC)...

Atopic dermatitis (AD) patients with skin barrier dysfunction are considered to be at a higher risk of allergic contact (ACD), although previous studies showed that attenuated ACD responses strong sensitizers in AD compared healthy controls. However, the mechanisms response attenuation unclear. Therefore, using hypersensitivity (CHS) mouse model, this study explored differences CHS hapten sensitization between NC/Nga mice or without induction (i.e., non-AD and mice, respectively). In study,...

We have previously proposed an excellent method to incorporate many electrochemical sensors into a single chip device using two sets of microelectrode array induce local redox cycling, and we designated the novel methodology as cycling-based (LR-EC) system. In this study, developed LRC-EC consisting 256 sensors. At sensors, ring-type interdigitated (IDA) electrodes were placed cycling. The was applied evaluate three-dimensional (3D) culture cells, such embryoid bodies (EBs).

Abstract Introduction Mechanisms underlying skin sensitization in allergic contact dermatitis have been actively studied using the murine hypersensitivity (CHS) model. However, much less is known about at vaginal mucosa (VM). Methods We developed a CHS model with VM and epicutaneous elicitation ear. then examined proliferation activity of lymphocytes, frequencies T cells differentiation hapten‐specific draining lymph nodes (dLNs) after sensitization. Results Hapten‐specific responses to...

Skin sensitization is a key adverse effect to be addressed during hazard identification and risk assessment of chemicals, because it the first step in development allergic contact dermatitis. Multiple non-animal testing strategies incorporating vitro tests silico tools have achieved good predictivities when compared with murine local lymph node assay (LLNA). The binary test battery KeratinoSensTM h-CLAT could used classify non-sensitizers as part bottom-up approach. However, quantitative for...

In this study, we have developed a novel electrochemical detection system containing many sensors. The is based on local redox cycling to incorporate sensors into small chip device. density of the highest in field lab-on-a-chip devices. By using device, comprehensive imaging can be achieved. device was applied for cell analysis three-dimensional culture cells.

Abstract not Available.