Eukaryotic RNA polymerase III (Pol III) relies on a transcription factor TFIIF-like Rpc37/53 subcomplex for promoter opening, elongation, termination, and reinitiation. By incorporating the photoreactive amino acid p-benzoyl-l-phenylalanine (BPA) into Rpc37, Rpc53, Rpc2 subunit of Pol III, we mapped protein-protein interactions, revealing position within preinitiation complex (PIC). BPA photo-cross-linking was combined with site-directed hydroxyl radical probing to localize dimerization...

Transcription initiation in all three domains of life requires the assembly large multiprotein complexes at DNA promoters before RNA polymerase (RNAP)-catalyzed transcript synthesis. Core RNAP subunits show homology among life, and recent structural information supports this homology. General transcription factors are required for productive complex formation. The archaeal general TATA-element-binding protein (TBP), which mediates promoter recognition, factor B (TFB), recruitment RNAP,...

Transcription initiation by eukaryotic RNA polymerase (Pol) III relies on the TFIIE-related subcomplex C82/34/31. Here we combine cross-linking and hydroxyl radical probing to position C82/34/31 around Pol active center cleft. The extended winged helix (WH) domains 1 4 of C82 localize clamp head core, respectively, two WH C34 span cleft from coiled-coil region protrusion. apparently cooperate with other mobile regions flanking during promoter DNA binding, opening, loading. Together published...

Hyperbilirubinemia is one of the most common causes for hospital admission in neonatal infants. Previous studies have found that jaundice may be initial symptoms related to urinary tract infection (UTI) This study evaluate incidence and factors infants with presentation hyperbilirubinemia final diagnosis UTI a tertiary teaching hospital.We retrospectively investigated medical records admitted younger than 8 weeks old between January December 2008. The jaundiced having tests urinalysis were...

Abstract Transcription factor IIB (TFIIB) is an essential component in the formation of transcription initiation complex eucaryal and archaeal transcription. TFIIB interacts with a promoter containing TATA‐binding protein (TBP) to facilitate interaction RNA polymerase II (RNA pol II) associated IIF (TFIIF). contains zinc‐binding motif near N‐terminus that directly involved II/ TFIIF plays crucial role selecting site. The solution structure N‐terminal residues 2–59 human was determined by...

TFIIB-related factor Brf1 is essential for RNA polymerase (Pol) III recruitment and open-promoter formation in transcription initiation. We site specifically incorporated a nonnatural amino acid cross-linker into to map its protein interaction targets the preinitiation complex (PIC). Our cross-linking analysis N-terminal domain of indicated pattern multiple interactions reminiscent TFIIB Pol active-site cleft. In addition TFIIB-like interactions, cyclin repeat subdomain contact with...

Retrotransposons are eukaryotic mobile genetic elements that transpose by reverse transcription of an RNA intermediate and derived from retroviruses. The Ty1 retrotransposon Saccharomyces cerevisiae belongs to the Ty1/Copia superfamily, which is present in every genome. Insertion into S. genome, occurs upstream genes transcribed Pol III, requires element-encoded integrase (IN) protein. Here, we report Ty1-IN interacts vivo vitro with III-specific subunits mediate insertion III-transcribed...

Microsomal triglyceride transfer protein (MTTP) is an endoplasmic reticulum resident that essential for the assembly and secretion of (TG)-rich, apoB-containing lipoproteins. Although function structure mammalian MTTP have been extensively studied, how exactly transfers lipids to lipid acceptors whether there are other biomolecules involved in MTTP-mediated transport remain elusive. Here we identify a role this process poorly characterized PRAP1. We report PRAP1 partially colocalized...

The general transcription factor IIH (TFIIH) plays crucial roles in as part of the pre-initiation complex (PIC) and DNA repair nucleotide excision (NER) machinery. During NER, TFIIH recruits 3′-endonuclease Rad2 to damaged DNA. In this manuscript, we functionally structurally characterized interaction between Tfb1 subunit Rad2. We show that deletion either PH domain (Tfb1PH) or several segments spacer region yield yeast with enhanced sensitivity UV irradiation. Isothermal titration...

We aimed to investigate the benefits and risks of dual antiplatelet therapy (DAPT) after coronary drug-eluting stent (DES) implantation in patients undergoing hemodialysis.A nested case-control analysis on hemodialysis receipt DES DAPT treatment was conducted using data from Taiwan's National Health Insurance Research Database for period 2007-2011. Cases myocardial infarction or death within 1 year were matched one-to-one with control patients. Odds ratios calculated compare continuation...

Sumoylation regulates a wide range of cellular processes. However, little is known about the regulation SUMO machinery. In this study, we demonstrate that two lysine residues (Lys-153 and Lys-157) in C-terminal region yeast E2-conjugating enzyme Ubc9 are major minor autosumoylation sites, respectively. Surprisingly, mutation Lys-157 (<i>ubc9</i>^K157R) significantly stimulates level at Lys-153. The functional role exemplified our findings cell cycle-dependent sumoylation...

Quadratic-congruence carrier-hopping prime codes (QC-CHPCs) with zero autocorrelation sidelobes, cross-correlation values of at most two, and expanded cardinality were recently constructed for wavelength-time "multicode-keying" optical code division multiple access (O-CDMA) improved data throughput obscurity. To support multimedia services different discrete bit-rate requirements, "multiple-length" QC-CHPCs are algebraically in this paper. In contrary to conventional single-length codes, our...

The RNA polymerase III (Pol III)-specific transcription factor Bdp1 is crucial to Pol recruitment and promoter opening in initiation, yet structural information sparse. To examine its protein-binding targets within the preinitiation complex at residue level, photoreactive amino acids were introduced into Saccharomyces cerevisiae Bdp1. Mutations highly conserved SANT domain cross-linked IIB (TFIIB)-related Brf1, consistent with findings of previous studies. In addition, we identified an...

<h3>Background</h3> Although recent clinical trials raised concerns about the risk for heart failure (HF) in dipeptidyl peptidase-4 (DPP-4) inhibitor use, data on cardiovascular risks patients with pre-existing HF are still lacking. <h3>Methods</h3> We used Taiwan9s National Health Insurance Research Database to identify 196 986 diagnosed type 2 diabetes mellitus (T2DM) who had previous history of between 2009 and 2013. This population included 30 204 DPP-4 users 166 782 propensity...

Managing cardiovascular disease (CVD) risk factors is the key to prevent CVD. This study aimed CVD by introducing asymptomatic meibomian gland dysfunction (MGD), a condition associated with various factors, as an early indicator for in middle-aged population. Participants and without MGD underwent standardized questionnaires, physical examinations, laboratory investigations. One ophthalmologist completed identification grading of using slit-lamp biomicroscopy examination on eyelid margins,...

<h3>Background:</h3> Angiotensin-converting-enzyme (ACE) inhibitors and angiotensin II receptor blockers (ARBs) are effective treatments for diabetic retinopathy, but randomized trials meta-analyses comparing their effects on macrovascular complications have yielded conflicting results. We compared the effectiveness of these drugs in patients with pre-existing retinopathy a large population-based cohort. <h3>Methods:</h3> conducted propensity score–matched cohort study using Taiwan's...

There are as many one-third of the systemic lupus erythematosus (SLE) patients who suffer from dry eye syndrome. To this date, syndrome in SLE is believed to be caused by secondary Sjogren (sSS). However, there increasing evidence for possible independency and sSS suffering autoimmune diseases. The purpose retrospective observational case series was identify without had syndrome, examine correlation different autoantibodies severity, determine cause these patients. We included 49 consecutive...

We propose a "synchronous" wavelength-time optical code-division multiple access (CDMA) scheme using 2D Walsh-based bipolar-bipolar code, in which Walsh code sequences are used for time spreading, place of Barker our previously proposed ldquoasynchronousrdquo code. The new synchronous coding supports larger cardinality and better performance than the asynchronous because has zero cross-correlational property at expense system synchronization. One potential application is future CDMA-based...