A5042481813- Retinal Diseases and Treatments
- Autophagy in Disease and Therapy
- Retinal Development and Disorders
- Retinal Imaging and Analysis
- Sirtuins and Resveratrol in Medicine
- Advanced Glycation End Products research
- Genomics, phytochemicals, and oxidative stress
- melanin and skin pigmentation
- Neuroinflammation and Neurodegeneration Mechanisms
- Ocular Diseases and Behçet’s Syndrome
- Glaucoma and retinal disorders
- Antioxidant Activity and Oxidative Stress
- Phytochemicals and Antioxidant Activities
- Endoplasmic Reticulum Stress and Disease
- Skin Protection and Aging
- Ubiquitin and proteasome pathways
- Retinoids in leukemia and cellular processes
- Genetics, Bioinformatics, and Biomedical Research
- Ocular Oncology and Treatments
- Adenosine and Purinergic Signaling
- Mosquito-borne diseases and control
- Calcium signaling and nucleotide metabolism
- Antimicrobial Resistance in Staphylococcus
- Lipid metabolism and biosynthesis
- Connective Tissue Growth Factor Research
University of Eastern Finland
2016-2025
Kuopio University Hospital
2014-2019
National Academy of Medicine
2017
Örebro University Hospital
2008
Örebro University
2007
Human morphology is a critical component of dental and medical graduate training. Innovations in basic science teaching methods are needed to keep up with an ever-changing landscape technology. The purpose this study was investigate whether students histology course would have better grades if they used gaming software Kahoot® gamification effects on learning enjoyment.
Age-related macular degeneration (AMD) is a multi-factorial disease that the leading cause of irreversible and severe vision loss in developed countries. It has been suggested pathogenesis dry AMD involves impaired protein degradation retinal pigment epithelial cells (RPE). RPE are constantly exposed to oxidative stress may lead accumulation damaged cellular proteins, DNA lipids evoke tissue deterioration during aging process. The ubiquitin-proteasome pathway lysosomal/autophagosomal two...
Abstract MicroRNAs (miRNAs) regulate gene expression; many of them act in the retinal pigment epithelium (RPE), and RPE degeneration is known to be a critical factor age‐related macular (AMD). Repeated injections with anti‐VEGFA (vascular endothelial growth A) are only effective therapy wet AMD. We investigated correlation between expression 18 miRNAs involved regulation VEGFA serum 76 AMD patients 70 controls. Efficacy treatment was evaluated by counting number delivered up 12 years. In...
Impaired autophagic and proteasomal cleansing have been documented in aged retinal pigment epithelial (RPE) cells age-related macular degeneration (AMD). Omega-3 fatty acids resveratrol many positive homeostatic effects RPE cells. In this work, ARPE-19 were treated with 288 ng of Resvega, containing 30 mg trans 665 omega-3 acids, among other nutrients, proteasome inhibitor MG-132 or autophagy bafilomycin A1 up to 48 h. Autophagy markers p62/SQSTM1 (p62) LC3 (microtubule-associated protein...
Increased oxidative stress and mitochondrial damage are observed in protein aggregation diseases, such as age-related macular degeneration (AMD). We have recently reported elevated levels of markers, damaged mitochondria, accumulating lysosomal lipofuscin extracellular drusen-like structures the retinal pigment epithelial cells (RPE) dry AMD-resembling NFE2L2/PGC1α double knockout (dKO) mouse model. Here, we provide evidence a disturbance autolysosomal machinery handling clearance RPE...
In diabetic patients, high blood glucose induces alterations in retinal function and can lead to visual impairment due retinopathy. immortalized pigment epithelial (RPE) cultures, concentrations are shown barrier properties. For the first time, induced pluripotent stem-cell-derived epithelium (hiPSC-RPE) cell lines derived from type 2 diabetics healthy control patients were utilized assess effects of concentration on cellular functionality. We show that both hiPSC-RPE differentiate mature...
Retinal pigment epithelial (RPE) cells maintain homeostasis at the retina and they are under continuous oxidative stress. Cigarette smoke is a prominent environmental risk factor for age-related macular degeneration (AMD), which further increases oxidant load in retinal tissues. In this study, we measured stress inflammatory markers upon cigarette smoke-derived hydroquinone exposure on human ARPE-19 cells. addition, studied effects of commercial Resvega product hydroquinone-induced...
Age-related macular degeneration (AMD) is the most prevalent form of irreversible blindness worldwide in elderly population. In our previous studies, we found that deficiencies nuclear factor, erythroid 2 like (NFE2L2) and peroxisome proliferator-activated receptor gamma coactivator 1-α (PGC-1α) genes caused AMD-like pathological phenotypes mice. present work, show hijacked epithelial-mesenchymal transition (EMT) due to common loss PGC-1α NFE2L2 (double knock-out, dKO) aged animals. The...
Degeneration and/or dysfunction of retinal pigment epithelium (RPE) is generally detected as the formation intracellular and extracellular protein aggregates, called lipofuscin drusen, respectively, in patients with age-related macular degeneration (AMD), leading cause blindness elderly population. These clinical hallmarks are linked to dysfunctional homeostasis inflammation furthermore, both regulated by changes Ca2+ concentration. While many other cellular mechanisms have been considered...
Purpose: Disturbed proteostasis leads to intracellular protein aggregation and decreased activity of autophagy seen in age‐related macular degeneration (AMD) Alzheimer's disease (AD). Autophagy is a cellular catabolic recycling process which degrades damaged aged organelles aggregates. Removed material sealed by autophagosome fuses with the lysosome form an autolysosome. However, research vivo challenging due lack specific markers follow autophagic structures i.e., formation autophagosomes...
Aims/Purpose: The 5xFAD mouse model of Alzheimer's disease overexpresses 42 amino acids long amyloid beta (Aβ42) found also from the drusen age‐related macular degeneration (AMD) patients. In this model, Aβ42 is produced in all tissues expressing precursor protein, including retinal pigment epithelial (RPE) cells, making it an interesting candidate for AMD. Ultrastructural changes RPE cells 5XFAD were examined. Methods: Six‐ and twelve‐months old mice wild type (WT) littermates ( n = 6)...
Aims/Purpose: Our previous studies have shown an increasing correlation between dysfunctional autophagy and the progression of age‐related macular degeneration (AMD). In this study, we investigated secretory autophagy, unconventional process involving secretion cytosolic leaderless cargos (proteins without N‐terminal peptides) into extracellular space. We analyzed signaling pathways in retinal pigment epithelial cells. Methods: Enucleated eyes from age‐matched controls ( n = 9), dry 6), wet...
RNA‐binding protein dysregulation and altered expression of proteins involved in the autophagy/proteasome pathway play a role many neurodegenerative disease onset/progression, including age‐related macular degeneration (AMD). HuR/ELAVL1 is master regulator gene human physiopathology. In ARPE‐19 cells exposed to proteasomal inhibitor MG132, HuR positively affects at posttranscriptional level p62 expression, stress response aggregate clearance with AMD. Here, we studied early effects...
Crowberry (Empetrum nigrum L.) is a relatively under-utilized wild berry that occurs widely throughout the northern hemisphere such as in Canada, Eurasia, and Europe. In this work, anthocyanins of crowberries were analyzed from four geographically distinct crowberry populations Finland using HPLC-DAD HPLC-ESI/MS/MS. A total number 15 detected; (11 structure elucidated) all samples order to profile-specific anthocyanin compositions Finland. The major found collected central eastern was...
The aim of the study was to investigate oxidative stress as well cellular protein accumulation in corneal diseases including keratoconus (KC), macular dystrophy (MCD), and Fuchs endothelial (FECD) at their primary affecting sites. Corneal buttons from KC, MCD, FECD patients, healthy controls, were analyzed immunohistochemically evaluate presence function proteostasis network. 4-Fydroxynonenal (4-HNE) used a marker stress, whereas levels catalase heat-shock 70 (HSP70) response antioxidant...