A5001258257- Inflammatory mediators and NSAID effects
- Eicosanoids and Hypertension Pharmacology
- Immune Response and Inflammation
- Lipid metabolism and biosynthesis
- Cardiac Fibrosis and Remodeling
- Liver Disease Diagnosis and Treatment
- Hormonal Regulation and Hypertension
- Advanced Glycation End Products research
- Epigenetics and DNA Methylation
- Renal and related cancers
- Kruppel-like factors research
- Cardiac Valve Diseases and Treatments
- Chronic Kidney Disease and Diabetes
- Nitric Oxide and Endothelin Effects
- Drug Transport and Resistance Mechanisms
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Estrogen and related hormone effects
- Pregnancy and preeclampsia studies
- Ion Transport and Channel Regulation
- Pulmonary Hypertension Research and Treatments
- Cholesterol and Lipid Metabolism
- RNA Research and Splicing
- Chemotherapy-induced organ toxicity mitigation
- Pregnancy and Medication Impact
East China Normal University
2023-2025
Dalian Medical University
2017-2023
Qiqihar University
2022
Zhejiang University
2022
Dalian University of Technology
2020
Institute for Advanced Medical Research
2020
Peking University
2008-2017
Affiliated Hospital of Nantong University
2016
Nantong University
2016
Lawson Health Research Institute
2009-2010
Prostaglandin E2 (PGE2) plays an important role in vascular homeostasis. Its receptor, E-prostanoid receptor 4 (EP4) is essential for physiological remodeling of the ductus arteriosus (DA). However, EP4 pathological remains largely unknown. We found that chronic angiotensin II (AngII) infusion mice with smooth muscle cell (VSMC)-specific gene knockout (VSMC-EP4-/-) frequently developed aortic dissection (AD) severe elastic fiber degradation and VSMC dedifferentiation. AngII-infused...
High-mobility group box 1 (HMGB1) is a nuclear protein that has been implicated in the myocardial inflammation and injury induced by ischemia-reperfusion (I/R). The purpose of present study was to assess role HMGB1 apoptosis I/R. In vivo, I/R an increase expression apoptosis. Inhibition (A-box) ameliorated I/R-induced vitro, isolated cardiac myocytes were challenged with anoxia-reoxygenation (A/R; vitro correlate I/R). A/R-challenged also generated underwent attenuated A/R-induced myocyte...
Abstract 17β-hydroxysteroid dehydrogenase-13 is a hepatocyte-specific, lipid droplet-associated protein. A common loss-of-function variant of HSD17B13 (rs72613567: TA) protects patients against non-alcoholic fatty liver disease with underlying mechanism incompletely understood. In the present study, we identify serine 33 17β-HSD13 as an evolutionally conserved PKA target site and its phosphorylation facilitates lipolysis by promoting interaction ATGL on droplets. Targeted mutation Ser33 to...
Sustained β-adrenergic activation induces cardiac fibrosis characterized by excessive deposition of extracellular matrix (ECM). Prostaglandin E2 (PGE2) receptor EP4 is essential for cardiovascular homeostasis. This study aims to investigate the roles cardiomyocyte (CM) and fibroblast (CF) in isoproterenol (ISO)-induced fibrosis. By crossing EP4f/f mice with α-MyHC-Cre or S100A4-Cre mice, this work obtains CM-EP4 knockout (EP4f/f-α-MyHCCre+) CF-EP4 (EP4f/f-S100A4Cre+) mice. The both genders...
Abstract High mobility group box 1 (HMGB1) is an alarmin actively secreted by immune cells and passively released necrotic nonimmune cells. HMGB1 has been implicated in both cardiac contractile dysfunction the lethality associated with sepsis/endotoxemia. The aim of current study was to assess whether viable cardiomyocytes could produce can affect myocardial contractility. LPS used as a model sepsis/endotoxemia mice isolated myocytes. increased expression vivo (immunohistochemistry)...
Formononetin, an isoflavone compound, has been extensively researched due to its various biological activities, including a potent protective effect on the cardiovascular system. However, impact of formononetin cardiac fibrosis not investigated. In this study, C57BL/6 mice were used establish animal models by subcutaneous injecting isoproterenol (ISO) and was orally administrated. The results showed that reversed ISO-induced heart stiffness revealed early-to-atrial wave ratio (E/A ratio)....
Among the four prostaglandin E2 receptors, EP3 receptor is one most abundantly expressed in white adipose tissue (WAT). The mouse gene gives rise to three isoforms, namely EP3α, EP3β, and EP3γ, which differ only at their C-terminal tails. To date, functions of its isoforms WAT remain incompletely characterized. In this study, we found that expression all were downregulated both db/db high-fat diet-induced obese mice. Genetic ablation (EP3−/− mice) or EP3α EP3γ with EP3β intact (EP3β led an...
Background/Aims: Transient lipid accumulation within hepatocytes preceding the peak proliferative process is a characteristic feature of liver regeneration. However, molecular mediators responsible for this and their functions are not well defined. Sterol regulatory element-binding proteins-1c (SREBP-1c) critical transcriptional factors that regulate homeostasis in liver. We hypothesized SREBP-1c deficiency induced alterations metabolism may influence hepatocyte proliferation Methods: 2/3...
Prostaglandin E2 and its cognate EP1-4 receptors play important roles in blood pressure (BP) regulation. Herein, we show that endothelial cell-specific (EC-specific) EP4 gene-knockout mice (EC-EP4-/-) exhibited elevated, while EC-specific EP4-overexpression (EC-hEP4OE) displayed reduced, BP levels compared with the control under both basal high-salt diet-fed conditions. The altered was completely abolished by treatment l-NG-nitro-l-arginine methyl ester (l-NAME), a competitive inhibitor of...
Background Mice with cardiomyocyte‐specific deletion of Bmal1, a core clock gene, had spontaneous abnormal cardiac metabolism, dilated cardiomyopathy, and shortened lifespan. However, the role cardiomyocyte Bmal1 in pressure overload induced remodeling is unknown. Here we aimed to understand contribution response by transverse aortic constriction or chronic angiotensin Ⅱ (AngⅡ) infusion. Methods Results By generating tamoxifen‐inducible knockout mouse line (cKO) challenging mice AngⅡ, found...
Hyperosmolarity of the renal medulla is essential for urine concentration and water homeostasis. However, how medullary collecting duct (MCD) cells survive function under harsh hyperosmotic stress remains unclear. Using RNA-Seq, we identified SLC38A2 as a novel osmoresponsive neutral amino acid transporter in MCD cells. Hyperosmotic stress-induced cell death occurred mainly via ferroptosis, it was significantly attenuated by overexpression but worsened Slc38a2 -gene deletion or silencing....
Methylglyoxal (MGO)-induced cell death in vascular endothelial cells (VECs) plays a critical role the progression of diabetic complications (DVCs). Previous studies have shown that MGO can induce inflammatory pyroptosis, leading to VEC damage. However, underlying mechanism remains unclear, and effective interventions are yet be developed. Human umbilical vein (HUVECs) were used for vitro experiments. Cell modes assessed through morphological observations. Mechanistic investigations performed...
Hyperuricemia (HUA) has attracted wide attention due to its close relationship with gout, hypertension, hypertriglyceridemia, obesity, atherosclerotic heart disease, type 2 diabetes and chronic kidney disease. Clinical observations suggest that people high levels of serum uric acid (sUA) exhibits impaired urine concentration. We speculate UA may regulate the expression AQPs through inflammatory pathways, resulting in renal revealed patients mice HUA had a polyuria phenotype found aquaporin...
Liver X receptors, including LXRα and LXRβ, are known to be master regulators of liver lipid metabolism. Activation increases hepatic storage in droplets (LDs). 17β-Hydroxysteroid dehydrogenase-13 (17β-HSD13), a recently identified liver-specific LD-associated protein, has been reported involved the development nonalcoholic fatty disease. However, little is about its transcriptional regulation. In present study, we aimed at determining whether 17β-HSD13 gene transcription controlled by LXRs....
Doxorubicin-driven cardiotoxicity could result in dilated cardiomyopathy and heart failure (HF). Previously, we showed that periplocymarin exerted a cardiotonic role by promoting calcium influx attenuating myocardial fibrosis induced isoproterenol (ISO) improving the metabolism of cardiomyocytes. However, impact on doxorubicin (DOX)-triggered has not been investigated. In current study, C57BL/6 mice were randomly divided into three groups, namely, control, DOX, DOX+periplocymarin groups. The...
Prostaglandin E2 receptor subtype 3 (EP3), a Gi protein-coupled activated by prostaglandin E2, plays particular role in cardioprotection. This study aimed to investigate the impact of EP3 deletion on cardiac remodeling and further elucidate related involvement possible signaling pathways.The animals used were knockout (EP3KO) mice wild-type (WT) litter mate controls at 16-18 weeks old. The high-resolution echocardiography weight index indicated that eccentric hypertrophy might occur EP3KO...
Krüppel-like transcription factor 7 (KLF7) promotes preadipocyte proliferation; however, its target gene in this process has not yet been identified. Using KLF7 ChIP-seq analysis, we previously showed that a KLF7-binding peak is present upstream of the cyclin-dependent kinase inhibitor 3 ( CDKN3) chicken preadipocytes. In study, identify CDKN3 as mediates effects on proliferation. Furthermore, 5'-truncating mutation analysis shows minimal promoter located between nt -160 and -7 (relative to...
Periplocymarin, which belongs to cardiac glycosides, is an effective component extracted from Periplocae Cortex. However, its cardiovascular effects remain unidentified. In the present study, injection of periplocymarin (5 mg/kg) through external jugular vein immediately increased mean arterial pressure (MAP) in anesthetized C57BL/6 mice. Ex vivo experiments using mouse mesenteric artery rings were conducted validate role on blood vessels. failed induce vasoconstriction directly, and had no...