A5034452359- CRISPR and Genetic Engineering
- Rice Cultivation and Yield Improvement
- Photosynthetic Processes and Mechanisms
- Cassava research and cyanide
- Genetics and Plant Breeding
- Chromosomal and Genetic Variations
- Photoreceptor and optogenetics research
- Genetic Mapping and Diversity in Plants and Animals
- Transgenic Plants and Applications
- Plant Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- Light effects on plants
- Isotope Analysis in Ecology
- Plant Molecular Biology Research
- Genetically Modified Organisms Research
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Plant responses to water stress
- Animal Genetics and Reproduction
- Plant nutrient uptake and metabolism
Cold Spring Harbor Laboratory
2025
University of California, Berkeley
2021-2024
Innovative Genomics Institute
2021-2024
Cornell University
2019-2020
Climate change imposes a severe threat to agricultural systems, food security, and human nutrition. Meanwhile, efforts in crop livestock gene editing have been undertaken improve performance across range of traits. Many the targeted phenotypes include attributes that could be beneficial for climate adaptation. Here, we present examples emerging applications research initiatives are aimed at improvement crops response change, discuss technical limitations opportunities therein. While only few...
Cassava (Manihot esculenta) is a starchy root crop that supports over billion people in tropical and subtropical regions of the world. This staple, however, produces neurotoxin cyanide requires processing for safe consumption. Excessive consumption insufficiently processed cassava, combination with protein-poor diets, can have neurodegenerative impacts. problem further exacerbated by drought conditions which increase this toxin plant. To reduce levels we used CRISPR-mediated mutagenesis to...
Understanding CRISPR-Cas9’s capacity to produce native overexpression (OX) alleles would accelerate agronomic gains achievable by gene editing. To generate OX with increased RNA and protein abundance, we leveraged multiplexed CRISPR-Cas9 mutagenesis of noncoding sequences upstream the rice PSBS1 gene. We isolated 120 gene-edited varying non-photochemical quenching (NPQ) in vivo—from knockout overexpression—using a high-throughput screening pipeline. Overexpression OsPsbS1 abundance two-...
Rice (Oryza sativa) is of paramount importance for global nutrition, supplying at least 20% calories. However, water scarcity and increased drought severity are anticipated to reduce rice yields globally. We explored stomatal developmental genetics as a mechanism improving resilience in while maintaining yield under climate stress. CRISPR/Cas9-mediated knockouts the positive regulator development STOMAGEN its paralog EPIDERMAL PATTERNING FACTOR-LIKE10 (EPFL10) yielded lines with ∼25% 80%...
Zeaxanthin (Zea) is a key component in the energy-dependent, rapidly reversible, nonphotochemical quenching process (qE) that regulates photosynthetic light harvesting. Previous transient absorption (TA) studies suggested Zea can participate direct via chlorophyll (Chl) to energy transfer. However, contamination of intrinsic exciton–exciton annihilation (EEA) makes assignment TA signal ambiguous. In this study, we present EEA-free data using Nicotiana benthamiana thylakoid membranes,...
Quantitative traits are important targets of both natural and artificial selection. The genetic architecture these its change during the adaptive process is thus fundamental interest. fate additive effects variants underlying a trait receives particular attention because they constitute variation component that transferred from parents to offspring governs response While estimation this phenotypic challenging, increasing availability dense molecular markers puts it within reach. Inbred plant...
The tools of genome editing were described more than a decade ago as promising ways to accelerate crop improvement in addition applications for human and animal health. Now, later, we are seeing across range different crops trait combinations that will bring benefits producers consumers. Countries around the world actively engaged updating regulatory frameworks govern this new technology adequately. In paper, describe recent advances tools, review select underway, consider technology, offer...
Cis-regulatory element editing can generate quantitative trait variation that mitigates extreme phenotypes and harmful pleiotropy associated with coding sequence mutations. Here, we applied a multiplexed CRISPR/Cas9 approach, informed by bioinformatic datasets, to genotypic in the promoter of OsSTOMAGEN, positive regulator rice stomatal density. Engineered corresponded broad continuous density, ranging from 70% 120% wild-type This panel variants was leveraged physiological assays establish...
Abstract Understanding CRISPR/Cas9’s capacity to generate native overexpression (OX) alleles would accelerate agronomic gains achievable by gene editing. To OX with increased RNA and protein abundance, we leveraged multiplexed CRISPR/Cas9 mutagenesis of non-coding DNA sequences located upstream the rice PSBS1 gene. We isolated 120 transgene-free, gene-edited varying NPQ in vivo —ranging from complete knockout overexpression, using a high-throughput phenotyping transgene screening pipeline....
Cassava (Manihot esculenta) is a globally important staple crop, particularly in sub-Saharan Africa. Its widespread consumption may be attributed to its hardiness. Relative other crops such as maize, wheat and rice, cassava produces more energy per unit area periods of drought marginal soils (Amelework et al., 2021). Furthermore, roots can stored below ground for extended time prior harvest, enabling greater management flexibility producers Thus, cultivation safeguard against food...
Abstract Rice ( Oryza sativa ) is of paramount importance for global nutrition, supplying at least 20% calories. However, water scarcity and increased drought severity are anticipated to reduce rice yields globally. stomatal developmental genetics were explored as a mechanism improve resilience while maintaining yield under climate stress. CRISPR/Cas9-mediated knockouts EPFL10 STOMAGEN yielded lines with c. 80% 25% wild-type density, respectively. epfl10 moderate reductions in densities able...
Abstract Zeaxanthin (Zea) is a key component in the energy-dependent, rapidly reversible, non-photochemical quenching process (qE) that regulates photosynthetic light harvesting. Previous transient absorption (TA) studies suggested Zea can participate direct via Chlorophyll (Chl) to energy transfer. However, contamination of intrinsic exciton-exciton annihilation (EEA) makes assignment TA signal ambiguous. In this study, we present EEA-free data using Nicotiana benthamiana thylakoid...
Abstract Quantitative traits are important targets of both natural and artificial selection. The genetic architecture these its change during the adaptive process is thus fundamental interest. fate additive effects variants underlying a trait receives particular attention because they constitute variation component that transferred from parents to offspring governs response While estimation this phenotypic challenging, increasing availability dense molecular markers puts it within reach....
Abstract Cis-regulatory element editing can generate quantitative trait variation while mitigating against extreme phenotypes and harmful pleiotropy associated with coding sequence mutations. Here, we applied a multiplexed guide RNA design approach, informed by bioinformatic datasets, to genotypic in the promoter of OsSTOMAGEN, positive regulator stomatal density rice. Engineered corresponded broad continuous density, ranging from 70% 120% wild-type density. This near-isogenic panel variants...