A5012358958- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Cancer Cells and Metastasis
- Digestive system and related health
- Animal Virus Infections Studies
- Escherichia coli research studies
- Mycobacterium research and diagnosis
- Genetic factors in colorectal cancer
- Bacterial Genetics and Biotechnology
- Pharmaceutical and Antibiotic Environmental Impacts
- Cancer therapeutics and mechanisms
- Respiratory viral infections research
- Immunotherapy and Immune Responses
- Clostridium difficile and Clostridium perfringens research
- Enterobacteriaceae and Cronobacter Research
University of Graz
2019-2023
Bernhard Nocht Institute for Tropical Medicine
2023
Significance Human gut microbes form a complex community with vast biosynthetic potential. Microbial products and metabolites released in the impact human health disease. However, defining causative relationships between specific bacterial disease initiation progression remains an immense challenge. This study advances understanding of functional capacity microbiota by determining presence, concentration, spatial temporal variability two enterotoxic produced gut-resident Klebsiella oxytoca ....
Abstract Klebsiella spp. that secrete the DNA-alkylating enterotoxin tilimycin colonize human intestinal tract. Numbers of toxigenic bacteria increase during antibiotic use, and resulting accumulation in lumen damages epithelium via genetic instability apoptosis. Here we examine impact this genotoxin on gut ecosystem. 16S rRNA sequencing faecal samples from mice colonized with oxytoca strains mechanistic analyses show is a pro-mutagenic affecting multiple phyla. Transient synthesis murine...
The DNA-alkylating metabolite tilimycin is a microbial genotoxin. Intestinal accumulation of in individuals carrying til+ Klebsiella spp. causes apoptotic erosion the epithelium and colitis. Renewal intestinal lining response to injury requires activities stem cells located at base crypts. This study interrogates consequences tilimycin-induced DNA damage cycling cells. We charted spatial distribution luminal quantities til metabolites Klebsiella-colonized mice context complex community. Loss...
Non-ribosomal peptides are one class of bacterial metabolites formed by gut microbiota. Intestinal resident Klebsiella oxytoca produces two pyrrolobenzodiazepines, tilivalline and tilimycin, via the same nonribosomal biosynthesis platform. These molecules cause human disease genotoxic tubulin inhibitory activities resulting in apoptosis intestinal epithelium, loss barrier integrity ultimately colitis. Here we report a fast, reliable, HPLC-HR-ESMS2 method for quantifying simultaneously...
ABSTRACT Objectives: Klebsiella oxytoca is a gastrointestinal pathobiont with the potential to produce toxins tilivalline and tilimycin, which cause antibiotic‐associated hemorrhagic colitis. Overgrowth of toxigenic K has recently been implicated in necrotizing enterocolitis. colonizes 2–9% healthy adults, however, there no systematic data on colonization children. We investigated its properties infants. Methods: sampled stool infants determined using culture PCR ( pehX ). Toxin was measured...