A5062403215- Arsenic contamination and mitigation
- Heavy Metal Exposure and Toxicity
- Trace Elements in Health
- Fluoride Effects and Removal
- Renal function and acid-base balance
- Heavy metals in environment
- Retinoids in leukemia and cellular processes
- Mercury impact and mitigation studies
- Selenium in Biological Systems
- Air Quality and Health Impacts
- Environmental Toxicology and Ecotoxicology
- Microbial Natural Products and Biosynthesis
- Cancer therapeutics and mechanisms
- Antibiotic Resistance in Bacteria
- Birth, Development, and Health
- Porphyrin and Phthalocyanine Chemistry
- Climate Change and Health Impacts
- Electrochemical Analysis and Applications
- Gut microbiota and health
- Fish Biology and Ecology Studies
- Biochemical and Molecular Research
- Aquaculture Nutrition and Growth
- Analytical chemistry methods development
- Thallium and Germanium Studies
- Biocrusts and Microbial Ecology
Columbia University
2021-2025
Center for Environmental Health
2021-2023
National Institute of Environmental Health Sciences
2021-2023
Nawi Graz
2016-2022
University of Graz
2014-2022
Abstract Background Carotenoids contribute significantly to animal body coloration, including the spectacular color pattern diversity among fishes. Fish, as other animals, derive carotenoids from their diet. Following uptake, transport and metabolic conversion, allocated coloration are deposited in chromatophore cells of integument. The genes involved these processes largely unknown. Using RNA-Sequencing, we tested for differential gene expression between carotenoid-colored white skin...
Background: Epigenetic dysregulation has been proposed as a key mechanism for arsenic-related cardiovascular disease (CVD). We evaluated differentially methylated positions (DMPs) potential mediators on the association between arsenic and CVD. Methods: Blood DNA methylation was measured in 2321 participants (mean age 56.2, 58.6% women) of Strong Heart Study, prospective cohort American Indians. Urinary species were using high-performance liquid chromatography coupled to inductively plasma...
BACKGROUND: Exposure to metals has been associated with cardiovascular disease (CVD) end points and mortality, yet prospective evidence is limited beyond arsenic, cadmium, lead. In this study, we assessed the association of urinary incident CVD all-cause mortality in a racially diverse population US adults from MESA (the Multi-Ethnic Study Atherosclerosis). METHODS: We included 6599 participants (mean [SD] age, 62.1 [10.2] years; 53% female) available at baseline (2000 2001) followed through...
Abstract The nonribosomal enterotoxin tilivalline was the first naturally occurring pyrrolobenzodiazepine to be linked disease in human intestine. Since producing organism Klebsiella oxytoca is part of intestinal microbiota and causes pathogenesis colitis it important understand biosynthesis regulation activity. Here we report show that this peptide assembly pathway initially generates tilimycin, a simple with cytotoxic properties. Tilivalline results from non‐enzymatic spontaneous reaction...
Significance Human gut microbes form a complex community with vast biosynthetic potential. Microbial products and metabolites released in the impact human health disease. However, defining causative relationships between specific bacterial disease initiation progression remains an immense challenge. This study advances understanding of functional capacity microbiota by determining presence, concentration, spatial temporal variability two enterotoxic produced gut-resident Klebsiella oxytoca ....
Arsenic-containing lipids (arsenolipids) are novel natural products recently shown to be widespread in marine animals and algae. Research interest these arsenic compounds lies their possible role the membrane chemistry of organisms and, because they occur many popular seafoods, human metabolism toxicology. Progress has been restricted, however, by lack standard arsenolipids a quantitative method for analysis. We report that certified reference material CRM 7405-a (Hijiki) is rich source...
Exposure to metals, a newly recognized risk factor for cardiovascular disease (CVD), could be related atherosclerosis progression.
Abstract Klebsiella spp. that secrete the DNA-alkylating enterotoxin tilimycin colonize human intestinal tract. Numbers of toxigenic bacteria increase during antibiotic use, and resulting accumulation in lumen damages epithelium via genetic instability apoptosis. Here we examine impact this genotoxin on gut ecosystem. 16S rRNA sequencing faecal samples from mice colonized with oxytoca strains mechanistic analyses show is a pro-mutagenic affecting multiple phyla. Transient synthesis murine...
Chronic arsenic exposure has been associated with an increased risk of cardiovascular disease; diabetes; cancers the lung, pancreas and prostate; all-cause mortality in American Indian communities Strong Heart Study.
Although enzymes have been found for many reactions, there are still transformations which no enzyme is known. For instance, not a single defined has described the reduction of C═N bond an oxime, only whole organisms. Such enzymatic oxime may give access to (chiral) amines. By serendipity, we that moiety adjacent ketone as well ester group can be reduced by ene-reductases (ERs) intermediate amino group. ERs well-known activated alkenes, α,β-unsaturated ketones. specific substrate used here,...
Analysis of essential and non-essential trace elements in urine has emerged as a valuable tool for assessing occupational environmental exposures, diagnosing nutritional status guiding public health care intervention. Our study focused on the analysis samples from Multi-Ethnic Study Atherosclerosis (MESA), precious resource research with limited sample volumes. Here we provide comprehensive sensitive method 18 using only 100 μL urine. Method sensitivity, accuracy, precision were assessed....
Background: Environmental metals are recognized risk factors for cardiovascular disease, yet the role of urinary and metal mixtures on incident heart failure (HF) remains understudied. Objectives: To evaluate prospective association HF across three geographically ethnically/racially diverse cohorts: Multi-Ethnic Study Atherosclerosis (MESA) Strong Heart (SHS) in United States, Hortega cohort Spain. Methods: A total 6,644, 2,917, 1,334 adults 35-80 years old were included MESA (39%...
Primary production in Mono Lake, a hypersaline soda lake rich dissolved inorganic arsenic, is dominated by Picocystis strain ML. We set out to determine if this photoautotrophic picoplankter could metabolize arsenic and doing so form unusual arsenolipids (e.g., bound 2-O-methyl ribosides) as reported other saline ecosystems halophilic algae. cultivated ML on seawater-based medium with either low (37 µM) or high (1000 phosphate the presence of arsenite (400 µM), arsenate (800 without...
Arsenic occurs in marine waters, typically at concentrations of 1-2 μg As kg-1, primarily as the inorganic species arsenate. Marine animals, however, contain extremely high levels arsenic (typically 2000-20 000 kg-1 wet mass), most which is present arsenobetaine, an organic form that has never been found seawater. We report a method based on ion-exchange preconcentration and HPLC/mass spectrometry to measure arsenobetaine seawater, apply samples seawater collected various depths from seven...
The influence of arsenate and phosphate levels in water on the formation arsenic-containing lipids (arsenolipids) water-soluble arsenicals by a unicellular marine alga was investigated exposing Dunaliella tertiolecta to five regimes arsenic phosphate, determining biosynthesized organoarsenicals with HPLC/mass spectrometry. Under all conditions, major arsenolipid produced D. novel phytyl 5-dimethylarsinoyl-2-O-methyl-ribofuranoside (AsSugPhytol546) representing ca. 35-65% total arsenolipids....
Lipid-soluble arsenic compounds, also called arsenolipids, are ubiquitous marine natural products of currently unknown origin and function. In our search for clues about the possible biological roles these we investigated metabolism in unicellular green alga Dunaliella tertiolecta, discovered an arsenolipid fundamentally different from all those previously identified; namely, a phytyl 5-dimethylarsinoyl-2-O-methyl-ribofuranoside. The discovery is particular interest because...
Background & aims: Gold nanoparticles (AuNPs) are useful tools for noninvasive drug delivery. AuNP nebulization has shown poor deposition results, and tracking postadministration involved methods inapplicable to clinical settings. The authors propose an intratracheal delivery method minimal loss computed tomography scans tracking. Materials methods: Through high-frequency directed postendotracheal intubation, the treated rats with AuNPs. Results conclusion: study showed a dose-dependent...
More than 700 million people worldwide suffer from diseases of the pancreas, such as diabetes, pancreatitis and pancreatic cancer. Often dysregulation potassium (K