A5053422116- Blood properties and coagulation
- Bacterial biofilms and quorum sensing
- Cardiac Ischemia and Reperfusion
- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Cardiac Fibrosis and Remodeling
- Cardiac Valve Diseases and Treatments
- Microplastics and Plastic Pollution
- Erythrocyte Function and Pathophysiology
- Vibrio bacteria research studies
- Cell Image Analysis Techniques
- Nanoparticles: synthesis and applications
- Nitric Oxide and Endothelin Effects
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Advanced Fluorescence Microscopy Techniques
- Cardiovascular Health and Disease Prevention
- Blood Coagulation and Thrombosis Mechanisms
- Heme Oxygenase-1 and Carbon Monoxide
- Cellular Mechanics and Interactions
- Recycling and Waste Management Techniques
University of Tübingen
2022-2025
Epithelial cells respond to infection with the intracellular bacterial pathogen Listeria monocytogenes by altering their mechanics promote collective infected cell extrusion (CICE) and limit spread across monolayers. However, underlying biochemical pathways remain elusive. Here, using in vitro (epithelial monolayers) vivo (zebrafish larvae) models of L. or Shigella flexneri, we explored role extracellular-signal-regulated kinase (ERK) activity waves coordinating mechanical battle between...
Cyclic guanosine monophosphate (cGMP) is a second messenger produced by the NO-sensitive guanylyl cyclase (NO-GC). The NO-GC/cGMP pathway in platelets has been extensively studied. However, its role regulating biomechanical properties of not yet addressed and remains unknown. We therefore investigated stiffness living after treatment with NO-GC stimulator riociguat or activator cinaciguat using scanning ion conductance microscopy (SICM). Stimulation human murine cGMP-modulating drugs...
ABSTRACT Objective Arterial stiffness, or loss of elastic compliance in large arteries, is an independent precursor cardiovascular disease (CVD) 1 and dementia 2 . Akin to anti-hypertensive lipid-lowering drugs, arterial de-stiffening therapies could be beneficial at decreasing CVD risk. We previously discovered that enhanced cytoskeletal actin polymerization vascular smooth muscle cells (VSMCs) contributes increased stiffness 3 In aortas VSMCs, we found decreased NO-sensitive guanylyl...
Platelet activation plays a critical role in thrombosis and hemostasis. Several pathophysiological situations lead to hemolysis, resulting the liberation of free ferric iron-containing hemin. Hemin has been shown activate platelets induce thrombo-inflammation. Classical antiplatelet therapy failed prevent hemin-induced platelet activation. Thus, aim present study was characterize mechanism death (ferroptosis). We evaluated vitro effect hemin on activation, signaling, oxylipins, plasma...
<p class="first" dir="auto" id="d5984679e198">Myocardial fibrosis is triggered, among other pathologies, by hypertension in conjunction with high angiotensin II (Ang II) levels. It defined excessive deposition of extracellular matrix (ECM) proteins such as collagen and the expression matricellular periostin (Postn). Cardiac myofibroblasts (CMF), a highly proliferative secretory cell type not found healthy hearts, are believed to be main source ECM proteins. As result, CMF contribute...
Abstract Cyclic guanosine monophosphate (cGMP) is a second messenger produced by the NO-sensitive guanylyl cyclase (NO-GC) enzyme. In platelets, NO-GC/cGMP pathway inhibits aggregation. One aspect of inhibitory mechanism involves changes in cytoskeleton; however, molecular mechanisms underlying platelet inhibition and its correlation with cytoskeletal cellular stiffness are poorly understood. We measured individual platelets after treatment NO-GC stimulator riociguat or activator cinaciguat,...