A5064962562- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive Biology and Fertility
- Sexual Differentiation and Disorders
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Wound Healing and Treatments
- Wnt/β-catenin signaling in development and cancer
- Photoreceptor and optogenetics research
- Epigenetics and DNA Methylation
- Molecular Communication and Nanonetworks
- Skin and Cellular Biology Research
- Neural dynamics and brain function
- Cardiovascular Effects of Exercise
- Neuroscience of respiration and sleep
- Single-cell and spatial transcriptomics
- Muscle Physiology and Disorders
- Cancer-related Molecular Pathways
- Migraine and Headache Studies
- Kruppel-like factors research
- Nicotinic Acetylcholine Receptors Study
- Cancer-related gene regulation
- Genetics and Physical Performance
- Genomics and Chromatin Dynamics
- Skin Diseases and Diabetes
- Signaling Pathways in Disease
Université Côte d'Azur
2013-2024
Inserm
2013-2024
Centre National de la Recherche Scientifique
2013-2024
Fédération Hospitalo-Universitaire, Paris Center for Microbiome Medicine
2021-2023
Laboratoire d'Excellence Canaux Ioniques d'Intérêt Thérapeutique
2021-2023
Centre Hospitalier Universitaire de Nice
2021-2023
Institut de Biologie Valrose
2002-2021
École Normale Supérieure
2009-2012
Astellas Pharma (South Korea)
2007
Victor (Japan)
2007
The sex of an individual is determined by the fate gonad. While expression Sry and Sox9 sufficient to induce male development, we here show that female differentiation requires activation canonical beta-catenin signaling pathway. controlled Rspo1 in XX gonads knockout mice masculinized gonads. Molecular analyses demonstrate absence female-specific Wnt4 as a consequence XY-like vascularization steroidogenesis. Moreover, germ cells embryos changes cellular adhesions failure enter specific...
Differentiation of germ cells into male gonocytes or female oocytes is a central event in sexual reproduction. Proliferation and differentiation fetal depend on the sex embryo. In mouse embryos, cell proliferation regulated by RNA helicase Mouse Vasa homolog gene factors synthesized somatic Sertoli promote gonocyte differentiation. female, ovarian requires activation WNT/β-catenin signaling pathway secreted protein RSPO1. Using models, we now show that Rspo1 also activates cells. XX Rspo1−/−...
Abstract Despite the importance of germ cell (GC) differentiation for sexual reproduction, gene networks underlying their fate remain unclear. Here, we comprehensively characterize expression dynamics during sex determination based on single‐cell RNA sequencing 14 914 XX and XY mouse GCs between embryonic days (E) 9.0 16.5. We found that diverge transcriptionally as early E11.5 with upregulation genes downstream bone morphogenic protein (BMP) nodal/Activin pathways in GCs, respectively. also...
Abstract Rhodopsins are ubiquitous light-driven membrane proteins with diverse functions, including ion transport. Widely distributed, they also coded in the genomes of giant viruses infecting phytoplankton where their function is not settled. Here, we examine properties OLPVR1 (Organic Lake Phycodnavirus Rhodopsin) and two other type 1 viral channelrhodopsins (VCR1s), demonstrate that VCR1s accumulate exclusively intracellularly, and, upon illumination, induce calcium release from...
Covering denuded dermal surfaces after injury requires migration, proliferation, and differentiation of skin keratinocytes. To clarify the major traits controlling these intermingled biological events, we surveyed genomic modifications occurring during course a scratch wound closure cultured human Using DNA microarray approach, report identification 161 new markers epidermal repair. Expression data, combined with functional analysis performed specific inhibitors ERK, p38MAPK...
The gonad arises from the thickening of coelomic epithelium and then commits into sex determination process. Testis differentiation is activated by expression Y-linked gene Sry, which promotes cell proliferation Sertoli cells, supporting cells testis. In absence Sry (XX individuals), activation WNT/CTNNB1 signalling, via upregulation Rspo1 Wnt4, ovarian differentiation. However, Wnt4 are expressed in early undifferentiated both sexes, Axin2-lacZ, a reporter canonical region E11.5 gonadal...
In mammals, male sex determination is governed by SRY-dependent activation of Sox9, whereas female development involves R-spondin1 (RSPO1), an activator the WNT/beta-catenin signaling pathway. Genetic analyses in mice have demonstrated Sry and Sox9 to be both required sufficient induce testicular development. These genes are therefore considered as master regulators Indeed, female-to-male reversal XX Rspo1 mutant correlates with expression, suggesting that this transcription factor induces...
In vertebrates, 2 main genetic pathways have been shown to regulate ovarian development. Indeed, a loss of function mutations in <i>Rspo1</i> and <i>Foxl2</i> promote partial female-to-male sex reversal. mice, it has that the secreted protein RSPO1 is involved differentiation transcription factor FOXL2 required for follicular formation. Here, we analysed potential interactions between these while expression seems be independent <i>Foxl2 </i>up-regulation,...
Retinoic acid synthesis is not essential for meiosis in fetal ovaries, challenging the dogma about meiosis-inducing factors.
By endowing light control of neuronal activity, optogenetics and photopharmacology are powerful methods notably used to probe the transmission pain signals. However, costs, animal handling ethical issues have reduced their dissemination routine use. Here we report LAKI (Light Activated K+ channel Inhibitor), a specific photoswitchable inhibitor pain-related two-pore-domain potassium TREK TRESK channels. In dark or ambient light, is inactive. alternating transdermal illumination at 365 nm 480...
Wnt-mediated signals are involved in many important steps mammalian regeneration. In multiple cell types, the R-spondin (Rspo) family of secreted proteins potently activates canonical Wnt/β-catenin pathway. Here, we identify Rspo1 as a mediator skeletal muscle tissue repair. First, show that deletion results global alteration regeneration kinetics following acute injury. We find progenitor cells lacking delayed differentiation due to reduced activation target genes. Furthermore, have fusion...
Abstract R-spondin2 (RSPO2) is a member of the R-spondin family, which are secreted activators WNT/β-catenin (CTNNB1) signaling pathway. In mouse postnatal ovary, WNT/CTNNB1 active in oocyte and neighboring supporting cells, granulosa cells. Although role Rspo2 has been previously studied using vitro experiments, results conflicting vivo ovarian function remains unclear. present study, we found that RSPO2/Rspo2 expression restricted to developing follicles both human ovaries from beginning...
Significance In the mammalian ovary, primordial germ cells maintain a genomic program associated with pluripotency until they stop proliferating, move toward oogenesis, and enter meiosis. The molecular mechanisms that enable to exit meiosis in timely manner are unclear, their identification represents major challenge reproductive biology because fertility of each individual depends on this. Evidence cessation cell proliferation is cell-autonomous event, unrelated number divisions, led search...
Previous studies have shown that σ receptors are overexpressed in tumor cells. However, the role of remains enigmatic. Recently, we and others demonstrated σ-1 receptor modulates K<sup>+</sup> channels pituitary. In present report, patch-clamp Western blot assays were used small cell lung cancer (SCLC, NCI-H209, NCI-H146) leukemic (Jurkat) lines to investigate effects ligands on voltage-gated proliferation. The (+)-pentazocine, igmesine, 1,3-di(2-tolyl)guanidine (DTG) all reversibly...
R-spondin1 is a secreted regulator of WNT signaling, involved in both embryonic development and homeostasis adult organs. It can have dual role, acting either as mitogen or tumor suppressor. During ovarian development, Rspo1 key factor required for sex determination differentiation the follicular cell progenitors, but downregulated after birth. In human, increased RSPO1 expression associated with carcinomas, it not clear whether cause consequence tumorigenic process. To address role ovaries,...
Activation and sensitization of trigeminal ganglia (TG) sensory neurons, leading to the release pro-inflammatory peptides such as calcitonin gene-related peptide (CGRP), are likely a key component in migraine-related headache induction. Reducing TG neuron excitability represents therefore an attractive alternative strategy relieve migraine pain. Here by using pharmacology genetic invalidation ex vivo vivo, we demonstrate that activating TREK1 TREK2 two-pore-domain potassium (K2P) channels...