A5018317661- Mass Spectrometry Techniques and Applications
- Chemical Synthesis and Analysis
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Chemical Reactions and Isotopes
- Renal Diseases and Glomerulopathies
- Amino Acid Enzymes and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Pregnancy and preeclampsia studies
- Click Chemistry and Applications
- Olfactory and Sensory Function Studies
- Birth, Development, and Health
- Biochemical Analysis and Sensing Techniques
- Isotope Analysis in Ecology
- Peptidase Inhibition and Analysis
- Pediatric Urology and Nephrology Studies
- Advanced Chemical Sensor Technologies
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Pregnancy and Medication Impact
- Erythrocyte Function and Pathophysiology
- Botulinum Toxin and Related Neurological Disorders
- Chronic Kidney Disease and Diabetes
- Nonlinear Optical Materials Research
- Salivary Gland Disorders and Functions
University of Wrocław
2015-2024
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2021
Faculty (United Kingdom)
2020
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread globally; recognition of immune responses to this virus will be crucial for disease 2019 (COVID-19) control, prevention and treatment. We comprehensively analysed IgG IgA antibody the SARS-CoV-2 nucleocapsid protein (N), spike domain 1 (S1) envelope (E) in: SARS-CoV-2-infected patient, healthy, historical pre-epidemic samples, including patients’ medical, epidemiological diagnostic data, virus-neutralizing capability...
Derivatization of peptides as quaternary ammonium salts (QAS) is a known method for sensitive detection by electrospray ionization tandem mass spectrometry.Hydrogens at α-carbon atom in N,N,N-trialkylglycine residue can be easily exchanged deuterons.The exchange reaction base-catalyzed and dramatically slow lower pH.Introduced deuterons are stable acidic aqueous solution not back-exchanged during LC-MS analysis.Increased efficiency, provided the fixed positive charge on QAS group, well...
Quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) is frequently based on the application of isotopically labeled standards which usually have to be specially synthesized. Although synthesis deuterated isotopologues relatively inexpensive, they are not considered as good internal due possible deuterium effect retention time during LC-MS analysis. We developed a method labeling denatonium benzoate (Bitrex) via H/D exchange its α-carbon hydrogen atoms in CH2 group...
Mimosine is a non-protein amino acid with various properties, such as antibacterial, anti-inflammatory, anti-cancer and anti-virus among others. Due to its structural similarity deferiprone (DFP), mimosine potential excellent metal chelator. In the present work, we combine experimental theoretical (DFT) approaches in order investigate properties of peptides. Six different peptides were synthesized their complex stoichiometry stability characterized by means UV-Vis spectrophotometry. Then,...
A straightforward cleavage method for N- acylated peptides based on the phenylthiohydantoin (PTH) formation is presented. The procedure could be applied to acid-stable resins, such as TentaGel HL–NH $$_{2}$$ . We designed a cleavable linker that consists of lysine residue with $$\upalpha $$ -amino group blocked by Boc, whereas $$\upvarepsilon used peptide synthesis. After assembly completed, protecting groups in side chains are removed using trifluoroacetic acid, thus liberating also linker....
Hydrogens connected to α-carbon (α-C) of amino acid residues are usually resistant hydrogen-deuterium exchange (HDX) unless reaction conditions promote racemization. Although N-methylglycine (sarcosine) residue has been found in biologically active peptide such as cyclosporine, the best our knowledge, HDX α-C protons this was not explored yet. Here, we presented a new and efficient methodology deuteration sarcosine under basic aqueous conditions. The deuterons, introduced at atom, do undergo...
A rapid and straightforward method for high-throughput analysis of single resin beads from one-bead-one-compound combinatorial libraries with high resolution electrospray ionization tandem mass spectrometry (HR ESI-MS/MS) is presented. The application an efficient peptide derivatization by quaternary ammonium salts (QAS) formation increases efficiency reduces the detection limit, allowing trace amounts compounds ESI-MS. Peptides, synthesized on solid support, contain a new cleavable linker...
The early asymptomatic stage of glomerular injury is a diagnostic challenge in the course renal and extra-renal disease, e.g., heart insufficiency. It was found that podocin, podocyte-specific protein present urine, may serve as biomarker diagnosis disease humans animals including glomerulonephritis, glomerulosclerosis, amyloidosis, or nephropathy. Therefore, there need development sensitive straightforward method urinary podocin identification. In this work, we report our extended research...
No sensitive method for diagnosing early kidney dysfunction in horses has been identified so far. Many studies carried out humans and small animals show that podocin can be useful to diagnose various diseases, mainly affecting the glomeruli. The aim of this study was perform a qualitative quantitative analysis urine samples obtained from healthy horses, with clinical at risk acute injury. objectives aimed assess: (1) whether selected tryptic peptide LC-MS-MRM allows detection horse; (2)...
Abstract Herein we present the synthesis of a novel type peptidomimetics composed repeating diaminopropionic acid residues modified with structurally diverse heterobifunctional polyethylene glycol chains (abbreviated as DAPEG). Based on developed compounds, library fluorogenic substrates was synthesized. Further deconvolution towards human neutrophil serine protease 4 (NSP4) yielded highly sensitive and selective internally quenched peptidomimetic substrates. In silico analysis obtained...
Recently, we developed a selective and efficient method of hydrogen-deuterium exchange (HDX) at the α-carbon (α-C) sarcosine residue (N-methylglycine) in model peptides [Bąchor et al. J. Mass Spectrom. 2014, 49, 43]. Here, report influence quaternary ammonium (QA) group on HDX α-C N-methylalanine peptides. The obtained results suggest significant acceleration caused by presence QA. effect depends distance between QA moiety. deuterons, introduced α-C, are resistant to back-exchange acidic...
Abstract The investigation on the synthesis of α‐C deuterated analogues cyclosporin A (CsA) in N ‐methylated amino acid residues was performed. reaction based our previous report (Bachor et al 2014 J Mass Spectrom 49, 43–49) proceeds presence bases like TBD or MTBD at pH 13.4. obtained results suggest possibility introduction 3 deuterons, 2 sarcosine, and 1 chiral atom 2‐N‐methyl‐(R)‐((E)‐2‐butenyl)‐4‐methyl‐L‐threonine residue. stability isotopologues analyzed both under neutral acidic...
Abstract Argireline‐containing cosmetics attract public interest due to their confirmed reduction of facial wrinkles. Argireline is a peptide that works by inhibiting the release neurotransmitters in neuromuscular junction, producing botox‐like effect. Therefore, it used as safe needle‐free alternative botox treatment. In this work we investigated presence cosmetic creams and sera application reversed phase liquid chromatography tandem mass spectrometry (RP‐HPLC/MS MS/MS). The analysis...
Armodafinil, the R enantiomer of modafinil, was approved in 2007 by US Food and Drug Administration as a wake-promoting agent for excessive sleepiness treatment. Due to its abuse students athletes, there is need quantification. Quantitative analysis liquid chromatography-mass spectrometry, however, though very common sensitive, frequently cannot be performed without isotopically labeled standards which usually have specially synthesized. Here we reported our investigation on preparation...
Chronic kidney disease (CKD) as one of the major public health problem itself and also strongly associated with cardiovascular diseases is a mortality factor in humans, well dogs cats. Recently, urinary podocin has been identified potential CKD biomarker. However, still sensitive straightforward method detection identification needed. Here we report our investigations on applicability liquid chromatography coupled tandem mass spectrometry analysis feline podocytes, based tryptic peptide...
High complexity of cell and tissue proteomes limits the investigation proteomic biomarkers. Therefore, methods enrichment some chemical groups peptides including thiopeptides are important tools that may facilitate analysis by reducing sample increasing proteome coverage. Here, we present a new method cysteine-containing tryptic peptide using commercially available TentaGel R RAM resin modified linker containing maleimide group, allowing thiol conjugation. The captured lysine residue were...
N ‐substituted glycines constitute mimics of natural amino acids that are great interest in the peptide‐based drug development. Peptoids‐oligo( glycines) have been recently demonstrated to be highly active peptidomimetics biological systems, resistant proteolytic degradation. We developed a method deuterium labeling containing glycine residues via H/D exchange their α ‐carbon hydrogen atoms. The was shown easy, inexpensive, and without use derivatization reagents or need for further...