A5044402466- Chemical Synthesis and Analysis
- Mass Spectrometry Techniques and Applications
- Supramolecular Self-Assembly in Materials
- Analytical Chemistry and Chromatography
- Advanced Proteomics Techniques and Applications
- Photochromic and Fluorescence Chemistry
- Protein Hydrolysis and Bioactive Peptides
- Radical Photochemical Reactions
- Sulfur-Based Synthesis Techniques
- Molecular Sensors and Ion Detection
- Metabolomics and Mass Spectrometry Studies
- Biochemical and Structural Characterization
- Pharmacological Effects and Assays
- Synthesis and Properties of Aromatic Compounds
- Synthesis and properties of polymers
- Click Chemistry and Applications
- Radiopharmaceutical Chemistry and Applications
- Redox biology and oxidative stress
- Origins and Evolution of Life
- Synthesis and Characterization of Heterocyclic Compounds
- Porphyrin and Phthalocyanine Chemistry
- Natural Antidiabetic Agents Studies
- Organoselenium and organotellurium chemistry
- Enzyme Structure and Function
- Chemical Reactions and Isotopes
University of Wrocław
2014-2025
Faculty (United Kingdom)
2020
Streptomyces smyrnaeus UKAQ_23, isolated from the mangrove-sediment, collected Jubail,Saudi Arabia, exhibited substantial antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA), including non-MRSA Gram-positive test bacteria. The novel isolate, under laboratory-scale conditions, produced highest yield (561.3 ± 0.3 mg/kg fermented agar) of compounds in modified ISP-4 agar at pH 6.5, temperature 35 °C, inoculum 5% v/w, 1.5% w/v, and an incubation period 7 days. two...
The difference in the crystal structure and growth kinetics of microtubes formed from l- d-enantiomers diphenylalanine dipeptide is investigated both experimentally theoretically by computer simulation. grown simultaneously under identical experimental conditions possess different crystallographic space groups, have essential sizes, demonstrate kinetics. Computer simulation molecular mechanics methods revealed a fundamental interaction between structural units chiralities. A model describing...
Abstract Mass spectrometric analysis of trace amounts peptides may be problematic due to the insufficient ionization efficiency resulting in limited sensitivity. One possible ways overcome this problem is application enhancers. Herein we developed new markers based on 2,4,6-triphenylpyridinium and 2,4,6-trimethylpyridinium salts. Using inexpensive commercially available pyrylium salt allows selective derivatization primary amino groups, especially those sterically unhindered, such as ε-amino...
Studies presenting visible-light-induced desulfurization of peptides containing a cysteine residue have been carried out. This transformation driven by light-emitting-diode-type light proceeds with high efficiency in an aqueous solution at room temperature and involves the use catalytic amount photosensitizer, Rose Bengal. The procedure has tested on model synthetic peptides, lysozyme C α-crystallin, successfully applied to one-pot native chemical ligation (NCL)–desulfurization protocol.
The diselenide bond has attracted considerable attention due to its ability undergo the metathesis reaction in response visible light. In our previous study, we demonstrated visible-light-induced of selenocysteine-containing linear peptides, allowing for convenient generation peptide libraries. Here, investigated transformation and cyclic peptides containing N-(2-selenoethyl)glycine moiety. were highly susceptible reaction, whereas systems gave only limited conversion yields product. both...
We present new tags based on the derivatives of phenylboronic acid and apply them for selective detection sugars peptide-sugar conjugates in mass spectrometry. investigated binding its quaternary ammonium salt (QAS) to carbohydrates peptide-derived Amadori products by HR-MS MS/MS experiments. The formation complexes between sugar or sugar-peptide synthetic was confirmed basis unique isotopic distribution resulting from presence boron atom. Moreover, incorporation a dramatically improved...
Peptides containing selenocysteine moieties are susceptible to non-catalytic reactions of diselenide bonds metathesis induced by visible light. In contrast previously reported radical disulfide bridges in cysteine derivatives, this newly developed reaction is fast and clean, proceeds without decomposition peptides formation side products. The bond was literature be more stable than the one also less thiols reducing reagents. We demonstrated that light induces Se-Se peptides. This important...
We report herein a novel ChemMatrix® Rink resin functionalised with two phenylboronate (PhB) moieties linked on the N-α and N-ε amino functions of lysine residue to specifically capture deoxyfructosylated peptides, compared differently glycosylated peptides in complex mixtures. The new PhB-Lys(PhB)-ChemMatrix® allows for exploitation previously demonstrated ability cis diols form phenylboronic esters. optimised capturing cleavage procedure from showed that only containing...
Lasso peptides are unique in that the tail of lasso peptide threads through its macrolactam ring. The unusual structure and biological activity have generated increased interest from scientific community recent years. Because this, many new types been discovered. These can be synthesized by microorganisms efficiently, yet, their chemical assembly is challenging. Herein, we investigated possibility high pressure inducing cyclization linear precursors peptides. Unlike other molecules like...
Abstract A new method of synthesis peptide conjugates with aromatic moieties substituted two sulfhydryl groups at 1,3‐positions is proposed. Amphiphilic peptides derivatized in such a way under oxidative conditions spontaneously form cyclic, covalent trimers and tetramers dominated by α‐helical conformations. The tendency to tri‐ or tetrahelical bundles depends on sequences the oxidation conditions, pH, additives.
The intramolecular interactions between the fructosyl moiety and phenylboronic acid incorporated into various positions of peptide chain were investigated using mass spectrometry (MS), circular dichroism (CD), nuclear magnetic resonance (NMR).
We developed a one-pot method for peptide cleavage from solid support via the N,S-acyl shift of N-2-[thioethyl]glycine and transthioesterification using external thiols to produce cyclic peptides through native chemical self-ligation with N-terminal cysteine. The feasibility this methodology is validated by syntheses model short peptides, including tetrapeptide, bicyclic sunflower trypsin inhibitor SFTI-1, rhesus Θ-defensin RTD-1. Synthesis whole precursor can be fully automated proceeds...
The aim of our research was to design an efficient method for the solid phase synthesis carbonylated peptides. For this purpose, we designed and synthesized a fully protected derivative Fmoc-amino(2,5,5-trimetyhyl-1,3-dioxolan-2-yl)acetic acid (Fmoc-Atda-OH) novel unnatural amino (Thr(O)-2-amino-3-oxo-butanoic acid). To obtain mentioned derivative, two synthetic strategies were investigated using different reagents carbonyl protection, ethane-1,2-diol 2,2-dimethyl-propane-1,3-diol....
The combination of deuterium–hydrogen exchange (DHX) and mass spectrometry (MS) can be used for studying a high pressure denaturation (HPD) proteins. Herein we present the results investigations influence glycation on HPD ubiquitin. Application various values causes different degrees protein unfolding, resulting in molecules with number protons available deuterons. dependence this gives information state protein. On basis obtained conclude that increasing fructosamine moieties ubiquitin...
The use of template molecules as chemical scaffolds that significantly influence the course reaction has recently been intensively studied. Peptide nucleic acids (PNA) are mimic natural acids. They a promising matrix in such reactions because they possess high affinity and specificity their interactions. manner PNA interaction is predictable based on sequence complementarity. Recently, we report visible light-induced metathesis peptides containing diselenide bond. Herein, present an...
Pathological levels of oxidative stress (OS) have been implicated in many diseases including diabetes mellitus, neurodegenerative diseases, inflammatory atherosclerosis, and cancer. Studies are however complicated by the low concentration oxidation products. To resolve this problem, we tested a new derivative aminoadipic semialdehyde (Fmoc-Aea-OH) solid-phase synthesis carbonylated peptides. We prepared series peptides with free acetylated N-terminal amino groups using Fmoc-Aea-OH reagent....
Peptides with diselenide bridge under UV irradiation eliminate one selenium atom forming selenoether bond good yield.
Two viologen-like macrocyclic receptors, [1]2+ and [2]2+, merging a bent oligophenylene loop with (para-xylylene)bispyridinium moiety were designed synthesized. Both dications derived from the same precursor: more...