A5022678830- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Virus-based gene therapy research
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Research and Treatments
- Glycosylation and Glycoproteins Research
- Cancer Genomics and Diagnostics
- Virology and Viral Diseases
- Cell Adhesion Molecules Research
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- vaccines and immunoinformatics approaches
- Cancer Cells and Metastasis
- Radiopharmaceutical Chemistry and Applications
- interferon and immune responses
- Cancer, Stress, Anesthesia, and Immune Response
- Inflammatory mediators and NSAID effects
- Proteoglycans and glycosaminoglycans research
- Immune Response and Inflammation
- Protease and Inhibitor Mechanisms
- Lymphoma Diagnosis and Treatment
- Viral Infections and Vectors
University Hospital Cologne
2023
Centrum für Integrierte Onkologie
2014-2023
DKFZ-ZMBH Alliance
1996-2015
German Cancer Research Center
2006-2015
Heidelberg University
2006-2015
Institute of Immunology
1976-2015
University Hospital Heidelberg
2002-2014
Lungenklinik Köln-Merheim
2011-2013
Technion – Israel Institute of Technology
2009
Science Foundation Ireland
2007
Rabbits primarily stimulated with a BSA (bovine serum albumin)-sulfanilic acid complex will produce good secondary response to the sulfanilic hapten if carrier used in stimulus is again BSA, and not HGG (human gamma globulin). In latter situation, obtained, however, rabbits are pretreated few weeks earlier free HGG. We conclude that immune involves recognition of determinants unrelated hapten. As receptors for probably situated on different cells, we suggest leading antibody formation...
Purpose Prognosis of patients with glioblastoma is poor. Therefore, in patients, we analyzed whether antitumor vaccination a virus-modified autologous tumor cell vaccine feasible and safe. Also, determined the influence on progression-free survival overall vaccination-induced reactivity. Patients Methods In nonrandomized study, 23 were vaccinated compared nonvaccinated controls (n = 87). Vaccine was prepared from patient's cultures by infection cells Newcastle Disease Virus, followed...
The proteins coded for by the HLA-A and HLA-B loci in man H-2K H-2D mice were identified as cell surface receptors Semliki Forest virus. This conclusion is based on following observations: (i) Water-soluble octamers of viral coat inhibit complement-dependent cytotoxicity antibodies directed against antigens mouse cells. (ii) Isolated detergent-soluble reconstituted lipid vesicles binding to human cells (as do water-soluble a lesser extent). (iii) Reconstituted interact solution with virus...
ABSTRACT The avian paramyxovirus Newcastle disease virus (NDV) selectively replicates in tumor cells and is known to stimulate T-cell-, macrophage-, NK cell-mediated responses. mechanisms of cell activation by NDV are poorly understood so far. We studied the expression ligand structures for activating receptors on NDV-infected cells. Upon infection with nonlytic strain Ulster lytic MTH-68/H, human carcinoma melanoma showed enhanced ligands natural cytotoxicity NKp44 NKp46, but not NKp30....
Abstract A syngeneic model system for the study of tumor metastases and cell‐mediated immunity is described. The consists two related, chemically induced murine lymphomas, non‐metastasizing parental line Eb its metastasizing variant ESb. An unrelated, (MDAY) included specificity controls. Serological typing revealed that both ESb were T lymphoid origin expressed H‐2K H‐2D molecules host strain DBA/2. By various electron microscope techniques, morphological differences observed between cell...
A plastic adherent variant line (ESb-M) of a highly invasive and metastatic murine T cell lymphoma (ESb) was found to have lost its potential while still being tumorigenic in normal syngeneic hosts. The retained most ESb-derived antigenic biochemical characteristics but differed at binding sites for certain lectins with specificity terminal N-acetylgalactosamine residues. Whereas such were masked by sialic acid on ESb cells, they became unmasked the line. Metastatic revertants ESb-M cells...
The present study emphasizes the importance of cell surface expression and secretion heparanase (endo-β- d -glucuronidase) in tumor angiogenesis metastasis. For this purpose, nonmetastatic Eb mouse lymphoma cells were transfected with predominantly intracellular human or a readily secreted chimeric construct composed enzyme chicken signal peptide. overexpressing invaded reconstituted basement membrane to much higher extent than enzyme. Cell invasion was inhibited presence laminaran sulfate,...
Previous studies with animal tumors showed that bone marrow (BM) is a privileged site where potentially lethal tumor cells are controlled in dormant state by the immune system. Here, we investigated BM of breast cancer patients respect to cell content, activation status and memory T-cell content. BM-derived from primary operated (n = 90) were compared those healthy donors 10) also respective blood samples. Cytokeratin 19-positive detected nested polymerase chain reaction. Three-color flow...
Abstract Pancreatic cancer is characterized by aggressive growth and treatment resistance. New approaches include immunotherapeutic strategies but the type extent of spontaneous immune responses against tumor antigens remains unclear. A dominance TH2 cytokines in patients' sera reported previously suggests systemic tumor-induced immunosuppression, potentially inhibiting induction tumor-reactive T cells. We localization, frequencies, functional potential spontaneously induced memory cells...
To investigate tumor-selective viral replication, we compared several tumorigenic human cell lines to nontumorigenic cells from the blood for sensitivity become infected by a recombinant lentogenic strain of Newcastle Disease Virus (NDV) with incorporated transgene EGFP (NDFL-EGFP). Although fluorescence signals in were only weak or missing completely, massive and long-lasting transgene-expression was observed all tumor lines. The majority (50-95%) could be infected, replication associated...