A5101835621- Geomechanics and Mining Engineering
- Geoscience and Mining Technology
- Rock Mechanics and Modeling
- Fluid Dynamics and Vibration Analysis
- Fluid Dynamics and Turbulent Flows
- Geotechnical and Geomechanical Engineering
- Microbial Metabolic Engineering and Bioproduction
- Fermentation and Sensory Analysis
- Fatigue and fracture mechanics
- Wind and Air Flow Studies
- Grouting, Rheology, and Soil Mechanics
- Hemoglobin structure and function
- Probiotics and Fermented Foods
- Food Quality and Safety Studies
- Magnetism in coordination complexes
- Landslides and related hazards
- Metal complexes synthesis and properties
- Innovative concrete reinforcement materials
- Enzyme Production and Characterization
- Proteoglycans and glycosaminoglycans research
- Tea Polyphenols and Effects
- Cytokine Signaling Pathways and Interactions
- Metallurgy and Material Forming
- Microbial bioremediation and biosurfactants
- Tailings Management and Properties
Jiangnan University
2021-2025
Tsinghua University
2022-2025
Jiaxing University
2024-2025
Shanghai University
2021-2024
Shanghai Institute of Technology
2019-2024
China Coal Research Institute (China)
2018-2023
China Tobacco
2021
China University of Mining and Technology
2015-2021
Institute of Mechanics
2021
Chinese Academy of Sciences
2021
The numerical investigation focuses on the flow patterns around a rectangular cylinder with three aspect ratios ( $L/D=5$ , $10$ $15$ ) at Reynolds number of $1000$ . study delves into dynamics vortices, their associated frequencies, evolution boundary layer and decay wake. Kelvin–Helmholtz (KH) vortices originate from leading edge (LE) shear transform hairpin vortices. Specifically, KH merge single LE vortex. However, $L/D=10$ two combine to form vortex, rapid formation vortex packets. A...
The electron transfer from NAD(P)H to heme is a rate-limiting step in the redox partner-mediated catalysis of P450 enzyme. However, due lack efficient engineering strategies, it difficult improve properties partner. Herein, we construct an effective approach modify partner for typical enzyme (OleP) that can catalyze stereoselective conversion lithocholic acid murideoxycholic acid. Firstly, combination computational modeling and experimental validation was performed rapidly identify most...
Abstract Background Surfactin produced by microbial fermentation has attracted increasing attention because of its low toxicity and excellent antibacterial activity. However, application is greatly limited high production costs yield. Therefore, it important to produce surfactin efficiently while reducing the cost. In this study, B . subtilis strain YPS-32 was used as a fermentative for surfactin, medium culture conditions were optimized. Results First, Landy 1 screened basal YPS-32. Then,...
The flow past a cylinder in proximity to plane wall is investigated numerically for small gap ratios. Three vortex dynamic processes associated with different hairpin generation mechanisms are identified the first time, and wake-induced turbulent transition analysed. shedding suppressed at $G/D = 0.1$ , while spanwise generated via Kelvin–Helmholtz instability evolves into vortices. For $G/D= 0.3$ upper lower rollers alternatively from cylinder, interact secondary vortex. split merges roller...
Introduction Soil salinization poses a worldwide challenge that hampers agricultural productivity. Methods Employing high-throughput sequencing technology, we conducted an investigation to examine the impact of compost on diversity bacterial communities in saline soils. Our study focused exploring inter-root soil plants following composting and subsequent addition Results Compared initial stage, Alpha results showed greater bacteria during rot stage. The germination index reaches 90%...
Potato common scab is caused by Streptomyces, which resides in soil and has become a serious disease potato planting areas worldwide. In this study, we obtained Bacillus subtilis YPS-32 strain natural screening, atmospheric room-temperature plasma (ARTP) mutagenesis field trial results showed that B. control efficacy of 83.70% against scab. The complete genome was sequenced, multiple genes related to the synthesis antibiotics plant growth promoters were detected. Based on genomic information...
Abstract Background The aging process in the tobacco production, as other food industries, is an important for improving quality of raw materials. In spontaneous aging, complex components flue-cured (FT) improve flavor or reduce harmful compounds through chemical reactions, microbial metabolism, and enzymatic catalysis. Some believed that tobacco-microbe played a significant part this process. However, little information available on how microbes mediate composition to FT, which will lay...
The flow past a cylinder near plane wall for small gap ratios (G/D=0.1, 0.3, and 0.9) fixed ReD = 1000 is numerically studied. fundamental features are characterized by the instantaneous mean fields. Then, dynamics of cylinder-wake/boundary-layer interaction revealed turbulent momentum transport kinetic energy production. fluctuations caused secondary vortex (SV) (at G/D=0.3, novel tertiary (TV) G/D=0.9) can be observed in distributions Reynolds stresses. For G/D=0.1 wake/boundary-layer...
Ovalbumin (OVA) is a high-quality protein for humans. Modifying microorganisms to produce proteins offers solution potential food shortages. In this study, OVA was expressed in
The electron transfer from NAD(P)H to heme is a rate‐limiting step in the redox partner‐mediated catalysis of P450 enzyme. However, due lack efficient engineering strategies, it difficult improve properties partner. Herein, we construct an effective approach modify partner for typical enzyme (OleP) that can catalyze stereoselective conversion lithocholic acid murideoxycholic acid. Firstly, combination computational modeling and experimental validation was performed rapidly identify most...
ABSTRACT Recombinant human haemoglobin (rHb) is a tetramer protein with heme as cofactors, which have extensive applications in the fields of biomaterials and biomedical therapeutics. However, due to poor structural stability, dissociation heme, weak oxygen transport efficiency, lower activity, utilisation rHb severely limited artificial carriers. Herein, based on novel developed high‐throughput screening strategies semi‐rational design, engineered mutant strong stability heme‐binding...
Alkali-stable amylases offer potential for integrating textile desizing and scouring processes. To meet industrial requirements, molecular engineering strategies were employed to enhance oxidative stability catalytic efficiency of alkaline amylase. In this study, alkali-stable amylase Amy I from Bacillus halodurans was engineered by modifying multiple highly flexible regions. The sequential truncation HFR I's N-terminal 40 residues yielded mutant T-40 with 77% higher residual activity than...
Currently, various types of myoglobins and hemoglobins are widely used in the fields food additives biocatalytic applications. However, limited availability heme constrains biosynthesis these high-activity hemoproteins microbial chassis cells. In this work, a new synthetic pathway was reconstructed mitochondria by eliminating spatial barrier during synthesis Saccharomyces cerevisiae, resulting significant enhancement intracellular supply. To further enhance supply essential precursor for...