A5005311821- RNA modifications and cancer
- MicroRNA in disease regulation
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
The Ohio State University
2022-2023
MicroRNAs (miRNAs) are about 22-nucleotide (nt) noncoding RNAs forming the effector complexes with Argonaute (AGO) proteins to repress gene expression. Although tiny (tyRNAs) shorter than 19 nt have been found bind plant and vertebrate AGOs, their biogenesis remains a long-standing question. Here, our in vivo vitro studies show several 3′→5′ exonucleases, such as interferon-stimulated 20 kDa (ISG20), three prime repair exonuclease 1 (TREX1), ERI1 (enhanced RNAi, also known 3′hExo), capable...
MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are loaded into Argonaute (AGO) proteins, forming RNA-induced silencing complexes (RISCs). The assembly process establishes the seed, central, 3' supplementary, tail regions across guide, enabling RISC to recognize target for silencing. This guide segmentation is caused by anchoring end at AGO PAZ domain, but minimum length required conformation remains be studied because current miRNA size defined Dicer processing ambiguous. Using a →...
MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are loaded into Argonaute (AGO) proteins, forming RNA-induced silencing complexes (RISCs). The assembly process establishes the seed, central, 3' supplementary, tail regions across guide, enabling RISC to recognize cleave target RNAs. This guide segmentation is caused by anchoring end at AGO PAZ domain, but minimum length required for conformation remains be studied because there was no method which do so. Using a 3'→5' exonuclease...
Abstract MicroRNAs (miRNAs) are about 22-nucleotide (nt) non-coding RNAs forming the effector complexes with Argonaute (AGO) proteins to repress gene expression. Although tiny (tyRNAs) shorter than 19 nt have been found bind plant and vertebrate AGOs, their biogenesis remains a long-standing question. Here, our in vivo vitro studies show several 3’→5’ exonucleases, such as interferon-stimulated 20 kDa (ISG20), three prime repair exonuclease 1 (TREX1), ERI1 (enhanced RNAi, also known 3’hExo),...