A5018408828- Drug-Induced Hepatotoxicity and Protection
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Liver Disease Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Drug Transport and Resistance Mechanisms
- MicroRNA in disease regulation
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer, Hypoxia, and Metabolism
- Liver physiology and pathology
- Alcohol Consumption and Health Effects
- Organ Transplantation Techniques and Outcomes
- Lipid Membrane Structure and Behavior
- Hepatitis B Virus Studies
- Herpesvirus Infections and Treatments
- Epigenetics and DNA Methylation
- Liver Disease and Transplantation
- Advanced biosensing and bioanalysis techniques
- Circular RNAs in diseases
- Neonatal Health and Biochemistry
- Pharmacogenetics and Drug Metabolism
- Nanopore and Nanochannel Transport Studies
- Erythrocyte Function and Pathophysiology
University of Pennsylvania
2023-2025
California University of Pennsylvania
2025
University of Massachusetts Chan Medical School
2022
The Ohio State University
2014-2021
Alpha-1 antitrypsin deficiency (AATD) is a rare autosomal codominant disease caused by mutations within the SERPINA1 gene. The most prevalent variant in patients PiZ SERPINA1, containing single G > A transition mutation. alpha-1 (AAT) prone to misfolding, leading accumulation of toxic aggregates hepatocytes. In addition, abnormally low level AAT secreted into circulation provides insufficient inhibition neutrophil elastase lungs, eventually causing emphysema. Cytosine and adenine base...
It is well established that the liver-specific miR-122, a bona fide tumor suppressor, plays critical role in lipid homeostasis. However, its role, if any, amino acid metabolism has not been explored. Since glutamine (Gln) energy and anaplerotic source for mammalian cells, we assessed Gln control wild type (WT) mice miR-122 knockout (KO) by stable isotope resolved metabolomics (SIRM) studies. Six-to eight-week-old WT KO 12- to 15-month-old liver tumor-bearing were injected with...
mRNA-based gene editing therapeutics offer the potential to permanently cure diseases but are hindered by suboptimal delivery platforms. Here, we devise a robust combinatorial chemistry for plug-and-play assembly of diverse biodegradable ionizable lipids and identify lead candidate that produces superior lipid nanoparticles various tools in vivo . Our study highlights utility this synthetic approach generality platform potent editing.
Crigler-Najjar syndrome is a rare disorder of bilirubin metabolism caused by uridine diphosphate glucuronosyl transferase 1A1 (UGT1A1) mutations characterized hyperbilirubinemia and jaundice. No cure currently exists; treatment options are limited to phototherapy, whose effectiveness diminishes over time, liver transplantation. Here, we evaluated the therapeutic potential systemically administered, lipid nanoparticle-encapsulated human UGT1A1 (hUGT1A1) mRNA therapy in mouse model. Ugt1...
Abstract Background & Aims Pharmacological activation of farnesoid X receptor (FXR) ameliorates liver injury, steatosis and inflammation in mouse models alcoholic disease (ALD), but the underlying mechanisms protective effect FXR against ALD remain unclear. Methods To investigate role ALD, we used NIAAA model chronic plus binge ethanol feeding FXR‐deficient knockout (FXR KO) mice. Results Ethanol‐mediated injury were increased KO mice, while both WT mice consumed same amount alcohol....
Although acetaminophen (APAP) is a widely used anti‐pyretic and analgesic, generally safe drug, it dose‐related toxin in all mammals causes ~1000 cases of acute liver failure (ALF) annually the United States. Acetaminophen toxicity major cause for N ‐acetylcysteine (NAC) remains only useful antidote APAP till now. MicroRNA‐122 (miR‐122) liver‐specific conserved microRNA that regulates metabolic homeostasis Hepatitis B C virus replication. It abundantly expressed adult expression levels many...
Abstract Hepatocellular carcinoma (HCC) is one of the most prevalent cancers worldwide with increasing incidence and mortality. Risk factors for this deadly disease include acquisition Hepatitis B or C virus, alcohol use, non-alcoholic steatohepatitis (NASH). Due to their ability regulate multiple genes therapeutic potential, miRNAs (miRs) have received considerable attention role as tumor suppressors oncogenes. In our previous study identify miRs involved in pathogenesis HCC, we...