A5035643335- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Influenza Virus Research Studies
- Drug-Induced Hepatotoxicity and Protection
- interferon and immune responses
- Liver Disease and Transplantation
- Glycosylation and Glycoproteins Research
- Endoplasmic Reticulum Stress and Disease
- Liver physiology and pathology
- Hepatitis C virus research
- Ubiquitin and proteasome pathways
- Cardiac Fibrosis and Remodeling
- Histone Deacetylase Inhibitors Research
- Monoclonal and Polyclonal Antibodies Research
- Diet, Metabolism, and Disease
- Alcoholism and Thiamine Deficiency
- Immunotherapy and Immune Responses
- Signaling Pathways in Disease
- Gout, Hyperuricemia, Uric Acid
- Caveolin-1 and cellular processes
- Liver Diseases and Immunity
- Inflammasome and immune disorders
- Chronic Lymphocytic Leukemia Research
- Antiplatelet Therapy and Cardiovascular Diseases
- Antimicrobial Peptides and Activities
Harvard University
2021-2025
Beth Israel Deaconess Medical Center
2021-2025
New York University
2017-2023
University of Otago
2015-2017
Cell death and inflammation play critical roles in chronic tissue damage caused by cholestatic liver injury leading to fibrosis cirrhosis. Liver cirrhosis is often associated with kidney damage, which a severe complication poor prognosis. Interferon regulatory factor 3 (IRF3) known regulate apoptosis inflammation, but its role cholestasis remains obscure. In this study.
Objective Alcohol use in metabolic dysfunction-associated steatohepatitis (MASH) is associated with an increased risk of fibrosis and liver-related death. Here, we aimed to identify a mechanism through which repeated alcohol binges exacerbate liver injury high fat-cholesterol-sugar diet (MASH diet)-induced model MASH. Design C57BL/6 mice received either chow or the MASH for 3 months without weekly binges. Neutrophil infiltration, neutrophil extracellular traps (NETs) were evaluated. Results...
Aim: Myocardial fibrosis is a well-established cause of increased myocardial stiffness and subsequent diastolic dysfunction in the diabetic heart. The molecular regulators that drive process fibrotic events heart are still unknown. We determined role microRNA (miR)-15 family remodelling Methods results: Right atrial appendage (RAA) left ventricular (LV) biopsy tissues collected from non-diabetic (ND) patients undergoing coronary artery bypass graft surgery showed significant down-regulation...
Binge drinking in obese patients positively correlates with accelerated liver damage and liver-related death. However, the underlying mechanism effect of alcohol use on progression metabolic-dysfunction-associated steatotic disease (MASLD) remain unexplored. Here, we show that short-term feeding a steatohepatitis (MASH) diet plus daily acute binges for three days induce injury activation NLRP3 inflammasome. We identify MASH promote inflammation via increased infiltration monocyte-derived...
Viruses dysregulate the host factors that inhibit virus infection. Here, we demonstrate human enzyme, histone deacetylase 1 (HDAC1) is a new class of factor inhibits influenza A (IAV) infection, and IAV dysregulates HDAC1 to efficiently replicate in epithelial cells. time-dependent decrease polypeptide level was observed IAV-infected cells, reducing <50% by 24 h further depletion (97%) expression RNA interference increased growth kinetics, increasing it >3-fold >6-fold 48 Conversely,...
Host cells produce variety of antiviral factors that create an state and target various stages influenza A virus (IAV) life cycle to inhibit infection. However, IAV has evolved strategies antagonize those factors. Recently, we reported a member class I host histone deacetylases (HDACs), HDAC1 possesses anti-IAV function. Herein, provide evidence HDAC2, another closely related in structure function, also properties. In turn, IAV, like HDAC1, dysregulates mainly at the polypeptide level...
Most chronic liver diseases progress to fibrosis, which, when left untreated, can lead cirrhosis and hepatocellular carcinoma. MicroRNA (miRNA)-targeted therapeutics have become attractive approaches treat diseases. In this study, we investigated the therapeutic effect of miR-155 inhibition in bile duct ligation (BDL) mouse model fibrosis evaluated role using miR-155-deficient (miR-155 knockout [KO]) mice. We found increased hepatic expression patients with BDL- CCl4-induced models fibrosis....
In a recent trial, patients with severe alcohol-associated hepatitis treated anakinra plus zinc (A+Z) had lower survival and higher acute kidney injury (AKI) rates versus prednisone (PRED). We characterize the clinical factors potential mechanisms associated AKI development in that trial.
Gold nanoparticles decorated with full-length sialic acid terminated complex bi-antennary N-glycans, synthesized glycans isolated from egg yolk, were used as a sensor for the detection of both recombinant hemagglutinin (HA) and whole influenza A virus particles H1N1 subtype. Nanoparticle aggregation was induced by interaction between termini attached to gold multivalent binding sites HA. Both dynamic light scattering (DLS) UV/Vis spectroscopy demonstrated efficiency sensor, which could...
Background and aims: Granulocyte colony-stimulating factor (G-CSF) has been proposed as a therapeutic option for patients with ACLF, however clinical outcomes are controversial. We aimed at dissecting the role of G-CSF in an alcohol-induced murine model ACLF. Methods : ACLF was triggered by single alcohol binge (5 g/kg) bile duct ligation (BDL) liver fibrosis model. A subgroup mice received two (200 μg/kg) or vehicle injections prior to acute decompensation alcohol. Liver, blood brain...
Abstract Background & Aims Pharmacological activation of farnesoid X receptor (FXR) ameliorates liver injury, steatosis and inflammation in mouse models alcoholic disease (ALD), but the underlying mechanisms protective effect FXR against ALD remain unclear. Methods To investigate role ALD, we used NIAAA model chronic plus binge ethanol feeding FXR‐deficient knockout (FXR KO) mice. Results Ethanol‐mediated injury were increased KO mice, while both WT mice consumed same amount alcohol....
Invited for this month's cover picture is the group of Professor Antony Fairbanks and his collaborators at University Canterbury Otago. The shows isolation complex bi-antennary oligosaccharides from hens' eggs their conjugation to gold nanoparticles. Gold nanoparticles carrying these sugars can then bind specific receptors (hemagglutinin) on surface influenza virus, causing particle aggregation, which changes spectroscopic properties. Upon they undergo a red-shift in plasmon resonance, as...
Severe alcohol-associated hepatitis (AH) is a life-threatening form of liver disease. Liver neutrophil infiltration hallmark AH, yet the effects alcohol on functions remain elusive. Identifying therapeutic targets to reduce neutrophil-mediated damage essential. Bruton’s tyrosine kinase (BTK) plays an important role in development and function; however, BTK AH unknown. Using RNA sequencing circulating neutrophils, we found increase Btk expression ( P = 0.05) phosphorylated (pBTK) patients...
Abstract Background Cholesterol-loading of mouse aortic vascular smooth muscle cells (mVSMCs) downregulates miR-143/145 , a master regulator the contractile state downstream TGFβ signaling. In vitro, this results in transitioning from mVSMC to macrophage-like state. This process likely occurs vivo based on studies and human atherosclerotic plaques. Objectives To test whether cholesterol-loading reduces VSMC signaling if cholesterol efflux will restore vitro . Methods Human coronary artery...