A5016904528- Liver physiology and pathology
- Organ Transplantation Techniques and Outcomes
- Pediatric Hepatobiliary Diseases and Treatments
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Renal and related cancers
- Cancer Genomics and Diagnostics
- Tissue Engineering and Regenerative Medicine
- Neonatal Respiratory Health Research
- Genetic and Kidney Cyst Diseases
- Renal Transplantation Outcomes and Treatments
- Oral and Maxillofacial Pathology
- Fibroblast Growth Factor Research
- Pancreatic function and diabetes
- Liver Disease and Transplantation
- Tuberculosis Research and Epidemiology
- Cystic Fibrosis Research Advances
- 3D Printing in Biomedical Research
- HIV/AIDS drug development and treatment
- Ion Transport and Channel Regulation
- Agriculture and Biological Studies
- Pancreatic and Hepatic Oncology Research
- Plant Reproductive Biology
- Gastrointestinal Tumor Research and Treatment
- Trace Elements in Health
- Hepatitis B Virus Studies
Erasmus MC
2019-2025
University Medical Center
2025
University Hospital and Clinics
2025
Rotterdam University of Applied Sciences
2025
University of Cambridge
2022-2023
Cambridge University Hospitals NHS Foundation Trust
2023
Wellcome/MRC Cambridge Stem Cell Institute
2022-2023
NIHR Cambridge Biomedical Research Centre
2022
Erasmus MC Transplant Institute
2021
Erasmus University Rotterdam
2019
Abstract The development, homeostasis, and repair of intrahepatic extrahepatic bile ducts are thought to involve distinct mechanisms including proliferation maturation cholangiocyte progenitor cells. This study aimed characterize human organoids (ECO) using canonical Wnt-stimulated culture medium previously developed for (ICO). Paired ECO ICO were derived from common duct liver tissue, respectively. Characterization showed both organoid types highly similar, though some differences in size...
ABSTRACT Hepatic organoid cultures are a powerful model to study liver development and diseases in vitro. However, hepatocyte-like cells differentiated from these organoids remain immature compared primary human hepatocytes (PHHs), which the benchmark field. Here, we applied integrative single-cell transcriptome chromatin accessibility analysis reveal gene regulatory mechanisms underlying differences. We found that, mature hepatocytes, activator protein 1 (AP-1) factors co-occupy regions...
Biliary disorders can lead to life-threatening disease and are also a challenging complication of liver transplantation. As there limited treatment options, tissue engineered bile ducts could be employed replace or repair damaged ducts. We explored how these constructs created by seeding hepatobiliary LGR5+ organoids onto tissue-specific scaffold. For this, we decellularized discarded human extrahepatic (EBD) that recellularized with different origin, is, biopsies, duct samples. Here,...
Cholangiocyte dysfunction accounts for a third of liver transplantations, access to which is limited by shortage healthy donor organs. A promising alternative the therapeutic use human induced pluripotent stem cell (hiPSC)-derived cholangiocytes. However, hiPSCs impeded lack knowledge regarding intrahepatic cholangiocyte development, limiting generation fully functional cells. In this study, we generate hiPSC-derived tubular cholangiocytes using an approach based in synthetic hydrogels....
Diseases of the bile duct (cholangiopathies) remain a common indication for liver transplantation, while little progress has been made over last decade in understanding underlying pathophysiology. This is largely due to lack proper vitro model systems study cholangiopathies. Recently, culture method developed that allows expansion human epithelial cells grown as extrahepatic cholangiocyte organoids (ncECOs) non-canonical Wnt-stimulating conditions. These ncECOs closely resemble...
BackgroundIn cystic fibrosis (CF), loss of CF transmembrane conductance regulator (CFTR)-dependent bicarbonate secretion precipitates the accumulation viscous mucus in lumen respiratory and gastrointestinal epithelial tissues. We investigated whether combination elexacaftor (ELX), ivacaftor (IVA) tezacaftor (TEZ), apart from its well-documented effect on chloride transport, also restores Phe508del-CFTR-mediated transport.MethodsEpithelial monolayers were cultured intestinal biliary...
Cholangiocytes express cystic fibrosis transmembrane conductance regulator (CFTR), which is involved in bicarbonate secretion for the protection against bile toxicity. During liver transplantation, prolonged hypoxia of graft associated with cholangiocyte loss and biliary complications. Hypoxia known to diminish CFTR activity intestine, but whether it affects cholangiocytes remains unknown. Thus, aim this study investigate effect on intrahepatic organoids (ICOs) test drug interventions...
Bile ducts are essential for bile transport and consist of complex branching tubular networks. Human patient-derived cholangiocyte develops a cystic rather than duct morphology. Here, we present protocol to establish morphogenesis in cholangiocarcinoma organoids. We describe steps the initiation, maintenance, expansion intrahepatic organoids This enables study organ-specific mesenchymal-independent provides an improved model biliary function diseases. For complete details on use execution...
Abstract Background and Aims Hepatic organoid cultures are considered a powerful model system to study liver development diseases in vitro . However, hepatocyte-like cells differentiated from such organoids remain immature compared primary human hepatocytes. Therefore, comprehensive understanding of differences gene regulatory mechanisms between hepatocytes hepatic is essential obtain functional for fundamental therapeutic applications. Methods We obtained at high purity all zones the lobule...
Organoid technology holds great promise for regenerative medicine but has not yet been applied to humans. With the groundbreaking paper in Science,(1) by Sampaziotis and colleagues from University of Cambridge, UK, clinical applications cholangiocyte organoids come into view. The authors describe how can be used context cholangiopathies show bile duct repair successfully transplanting these human liver. Using single-cell RNA sequencing, they furthermore demonstrate transcriptional diversity...