Ensiling native grass is an effective method to protect the nutritional quality of forage and alleviate feed shortages in cold winter Inner Mongolian Plateau. To improve usability resources as China, effects lactic acid bacteria molasses additions on microbial population, fermentation quality, during silage were investigated. Treatments a control treatment with no additive (CK), (L), (M), combination (L+M), all which stored at ambient temperature (17-28°C) for 7, 14, 30, 60 days. The results...

In this study, the ultrasound-assisted extraction (UAE) conditions of flavonoids from Lactuca indica L.cv. Mengzao (LIM) leaves was optimized, and content their antioxidant potential in different parts were analyzed. The optimal parameters to obtain highest total (TFC) a ratio liquid solid 24.76 mL/g, ultrasonic power 411.43 W, ethanol concentration 58.86% an time 30 min, average TFC LIM could reach 48.01 mg/g. For yield flavonoids, UAE method had best capacity compared with solvent...

In this study, we analyzed the fermentation quality, microbial community, and metabolome characteristics of ryegrass silage from different harvests (first harvest-AK, second harvest-BK, third harvest-CK) correlation between fermentative bacteria metabolites. The bacterial community metabolomic were by single-molecule real-time (SMRT) sequencing ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS), respectively. After 60 days ensiling, pH BK was significantly lower...

L. cv. Mengzao, a medicinal plant of the Ixeris genus, is rich in flavonoids. In order to thoroughly analyze distribution and dynamic change major flavonoids its various parts from different growth periods, extracted

This study aimed to investigate the effects of different growth stages (booting period-SYK; initial flowering-SCK; full flowering-SSK) on fermentation quality, microbial community, metabolic pathways and metabolomic characteristics Italian ryegrass silage.Single molecule real-time (SMRT) sequencing ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) were used analyze bacterial communities metabolites, respectively.After 60 d fermentation, SYK had lowest pH highest...

Selenium promotes plant growth and improves nutritional quality, the role of nano-selenium in alfalfa regulating quality is unknown. In this study, using 15N labeling method, it was found that could promote nitrogen metabolism photosynthesis by increasing light energy capture capacity activities key enzymes process, leading to an increase accumulation dry matter content. The transcriptome metabolome revealed mainly affected pathways 'biosynthesis amino acids', 'starch sucrose metabolism',...

Better quality and odor of silage normal microbial fermentation metabolism are mostly dependent on an appropriate moisture content. The purpose this study was to determine the effects different content gradients (50, 60, 70, 80%) bacterial community, odor, alfalfa at 60 days by using gas chromatography-mass spectrometry (GC-MS) electronic nose, with six replicates per group. results showed that there were significant differences in response intensity among all groups, which 80% group had...

Leymus chinensis (Trin.) Tzvel is a perennial high-quality indigenous grass in China; characterized by high yield, elevated crude protein content, excellent palatability, substantial leaf volume, adaptability, and longevity. This study aimed to examine the impact of Lactiplantibacillus plantarum (LP), Lactobacillus buhneri (LB), their combination (LPLB), complex enzyme preparation (CE) on quality microbial community silage. Throughout silage fermentation, pH levels decreased all treatment...

The objective of this study was to determine the effect Lactobacillus plantarum (L. plantarum) and propionic acid (PA) on microbial community fermentation performance high-moisture amaranth silage.Amaranth silages were rown without addition (AhGCK) as a control with L. JYLP-002 (AhGLP) or (AhGPA) then opened after 60 days ensiling quality.Crude protein (CP) content, lactic (LA) bacteria (LAB) counts significantly higher in AhGLP AhGPA compared those AhGCK (p < 0.05). In contrast, pH, acetic...

Harvest time and storage had significant effects on nutrient quality Volatile Organic Compounds (VOCs) of alfalfa. The objective this study was to use headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) analyze alfalfa at different harvest periods (budding stage, early blooming, full blooming), for 0 d, 90 180 270 360 the dynamic changes in VOCs nutritional quality. Results indicated that ketones, aldehydes, alcohols esters were main volatile components...

Hydrothermal biochar has demonstrated potential in enhancing crop growth by improving soil properties and microbial activity; however, its effectiveness varies with application rate, excessive amounts potentially inhibiting plant growth. This study employed a pot experiment approach to compare varying rates of hydrothermal (ranging from 0 50 t/ha) analyze effects on alfalfa biomass, photosynthetic efficiency, nutrient content, community composition. Biochar increased dry weight 12.22-21.20%...

In this study, the triterpenoids in leaves of Lactuca indica L.cv. Mengzao (LIM) were extracted via microwave-assisted ethanol extraction, and optimum extraction conditions for determined through single-factor experiments Box–Behnken method. The effects three factors (solid–liquid ratio, microwave power time) on total content (TTC) evaluated. TTC different parts (roots, stems, flowers) LIM growth stages was studied, scavenging highest DPPH, ABTS hydroxyl free radicals investigated. results...

Introduction Italian ryegrass is less studied in northern China due to high-quality forage grass has not been fully utilized. Full utilization of helps alleviate the shortage winter and spring season guarantee stable development livestock production. Consequently, this study was aimed evaluate effects different regions on fermentative products, bacterial community compositions, metabolic pathways metabolites silage. Methods The harvested from three (Ordos-WK; Hohhot-AK; Ulanqab-SYK) ensiled...

In this study, alfalfa silage with different packing densities (500 kg/m3, 600 kg/m3 and 700 kg/m3) were set up under outdoor high temperature indoor normal temperature. The chemical composition, fermentation quality microbial community of analyzed. results showed that DM WAC content decreased the increase density. When density is 600kg/m3, CP reaches maximum ADF lowest. Temperature has a certain effect on silage. Lactobacillus plantarum fermented at lower than At same time, However, was...

Lactuca indica L. cv. Mengzao (LIM), acknowledged as a pivotal "One Root of Medicine and Food", boasts dual applications in both culinary medicinal domains. This research delves into the influence various harvest periods (vegetative, budding, blossom, fruiting) on distinct parts (roots, stems, leaves, flowers, seeds) LIM, employing plant metabolomics to assess its chemical constituents. A total 66 constituents were identified with 11 components emerging potential markers for distinguish...

Lactuca indica L. contains high amounts of flavonoids and phenolic acids. However, there is limited information on the composition these compounds in different parts plant. The present study analyzed secondary metabolite profiles stem, leaf, flower, seed indicaL.cv. Mengzao (LIM) using a widely targeted metabolomic approach. A total 576 metabolites were identified, including 218, 267, 232, 286, 302, 308 differentially accumulated (DAMs) stem_vs_leaf, stem_vs_flower, stem_vs_seed,...

Abstract Aims The aim of this study was to investigate the dynamics bacterial communities and natural fermentation quality in three steppe types [meadow (MS); typical (TS); desert (DS)] on Mongolian Plateau. Methods results PacBio single molecule with real-time sequencing technology applied provide insights into physicochemical characteristics complex microbiome native grass after 1, 7, 15, 30 days process. dry matter, crude protein, water soluble carbohydrate (WSC) contents groups slowly...

Lactuca indica L. cv. Mengzao (LIM) has both edible and medicinal uses. Its leaves is used as vegetables, but the whole plant important value. In order to explore effects of different harvest periods on parts LIM, chemical constituents LIM were evaluated using ultrahigh-performance liquid chromatography coupled with quadrupole tandem Q exactive mass spectrometry (UPLC-QE-MS/MS). 66 detected, 21 components identified potential markers distinguish LIM. Furthermore, sensitive UPLC-MS/MS method...

Lactuca indica L. cv. Mengzao (LIM) is classified as one of the “One Root Medicine and Food”, it has both edible medicinal uses. To exploring effects in different harvest periods on parts LIM, chemical constituents LIM were evaluated based plant metabolomics. 66 detected, 11 components identified potential markers to distinguish parts. Among nutritional organs contain highest levels cichoric acid, rutin chlorogenic acid. Particularly, leaves at budding stage chicoric acid content 11.70 mg...