A5031802922- Renal Diseases and Glomerulopathies
- Dialysis and Renal Disease Management
- Vasculitis and related conditions
- Chronic Kidney Disease and Diabetes
- Angiogenesis and VEGF in Cancer
- Systemic Sclerosis and Related Diseases
- Autoimmune Bullous Skin Diseases
- Cell Adhesion Molecules Research
- Proteoglycans and glycosaminoglycans research
- Platelet Disorders and Treatments
- Renal Transplantation Outcomes and Treatments
- Renal and Vascular Pathologies
- Geology and Paleoclimatology Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Glycosylation and Glycoproteins Research
- Central Venous Catheters and Hemodialysis
- Complement system in diseases
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Monoclonal and Polyclonal Antibodies Research
- Parathyroid Disorders and Treatments
- Transplantation: Methods and Outcomes
- Renal and related cancers
- Acute Kidney Injury Research
- Inflammatory mediators and NSAID effects
- Erythropoietin and Anemia Treatment
University of Manchester
2015-2025
Manchester Academic Health Science Centre
2014-2025
Manchester University NHS Foundation Trust
2006-2025
Manchester Royal Infirmary
2012-2023
Wellcome Centre for Cell-Matrix Research
2020-2023
RELX Group (United States)
2017-2021
Landscape Research Group
2021
RELX Group (Netherlands)
2021
Cardiovascular Research Center
1996-2019
Devices for Dignity
2019
Antineutrophil cytoplasmic antibody (ANCA)–associated vasculitis is a severe condition encompassing two major syndromes: granulomatosis with polyangiitis (formerly known as Wegener's granulomatosis) and microscopic polyangiitis. Its cause unknown, there debate about whether it single disease entity what role ANCA plays in its pathogenesis. We investigated genetic basis.
The term Anthropocene, proposed and increasingly employed to denote the current interval of anthropogenic global environmental change, may be discussed on stratigraphic grounds.A case can made for its consideration as a formal epoch in that, since start Industrial Revolution, Earth has endured changes sufficient leave signature distinct from that Holocene or previous Pleistocene interglacial phases, encompassing novel biotic, sedimentary, geochemical change.These changes, although likely...
Idiopathic membranous nephropathy is a major cause of the nephrotic syndrome in adults, but its etiologic basis not fully understood. We investigated genetic biopsy-proven cases idiopathic white population.We performed independent genomewide association studies single-nucleotide polymorphisms (SNPs) patients with from three populations ancestry (75 French, 146 Dutch, and 335 British patients). The were compared racially matched control subjects; population stratification quality controls...
B-cell anomalies play a role in the pathogenesis of membranous nephropathy. depletion with rituximab may therefore be noninferior to treatment cyclosporine for inducing and maintaining complete or partial remission proteinuria patients this condition.We randomly assigned who had nephropathy, at least 5 g per 24 hours, quantified creatinine clearance 40 ml minute 1.73 m2 body-surface area been receiving angiotensin-system blockade 3 months receive intravenous (two infusions, 1000 mg each,...
The phospholipase A2 receptor (PLA2R) is the major target antigen in idiopathic membranous nephropathy. technique for measuring antibodies against PLA2R and relationship between antibody titer clinical characteristics are not well established. Here, we measured anti-PLA2R (aPLA2R) subclass a defined cohort of 117 Caucasian patients with nephropathy nephrotic-range proteinuria using both indirect immunofluorescence testing (IIFT) ELISA. We assessed agreement tests correlated baseline...
Phospholipase A2 receptor 1 (PLA2R) is a target autoantigen in 70% of patients with idiopathic membranous nephropathy. We describe the location major epitope N-terminal cysteine-rich ricin domain PLA2R that recognized by 90% human anti-PLA2R autoantibodies. The was sensitive to reduction and SDS denaturation isolated larger fragment containing ricin, fibronectin type II, first second C-type lectin domains (CTLD). However, nondenaturing conditions protected against fragments, including...
1. Dysregulated vascular endothelial growth factor (VEGF) expression has been reported in several pathological states based upon evidence of elevated serum VEGF levels. Using two immunoassays for VEGF, this study determines normal plasma and ranges, which are more likely to reflect circulating levels investigates a potential contribution from platelets detected serum. 2. The presence soluble receptor, sflt-1, at molar excess 7:1 significantly reduced measured both assays. Serum were higher...
Diabetic retinopathy and nephropathy cause significant morbidity in patients with diabetes. Vascular endothelial growth factor (VEGF) is a potent angiogenic vascular permeability implicated both of these diabetes complications. We previously reported transfection studies showing the VEGF −460 +405 polymorphisms to increase basal promoter activity by 71% compared wild-type sequence. Therefore, we investigated association proliferative diabetic nephropathy. DNA was isolated from 267 U.K....
Tumour vascularity and vascular endothelial growth factor (VEGF) expression were studied in 41 primary brain tumours of astrocytic oligodendroglial origin, order to define the potential role VEGF vascularization these tumours.Two commercial monoclonal antibodies protein (from R&D Systems NeoMarkers), raised against different isoforms, utilized. Each antibody consistently detected cell types. The only produced surface staining cells tumour capillaries, whereas with Neomarkers was largely...
The glomerulus contains unique cellular and extracellular matrix (ECM) components, which are required for intact barrier function. Studies of the components have helped to build understanding glomerular disease; however, full composition regulation ECM remains poorly understood. We used mass spectrometry-based proteomics enriched extracts a global analysis human in vivo identified tissue-specific proteome 144 structural regulatory proteins. This catalog includes all previously plus many new...
Background The optimal timing and duration of immunosuppressive therapy for idiopathic membranous nephropathy (iMN) have been debated. This study aimed to evaluate whether measuring the antibody against phospholipase A2 receptor (PLA2R-ab) at start end predicts long-term outcome therefore may inform this debate. Design, setting, participants, & measurements observational included all consecutive high-risk patients with progressive iMN observed from 1997 2005 treated oral cyclophosphamide...
The M-type receptor for phospholipase A2 (PLA2R1) is the major target antigen in idiopathic membranous nephropathy (iMN). Our recent genome-wide association study showed that genetic variants an HLA-DQA1 and allele associate most significantly with biopsy-proven iMN, suggesting rare within coding region of PLA2R1 gene may contribute to antibody formation. Here, we sequenced a cohort 95 white patients iMN assessed all 30 exons PLA2R1, including canonical (GT-AG) splice sites, by Sanger...
Mast cells, when activated, secrete a large number of fibrogenic factors and have been implicated in the development fibrotic conditions liver, lung, skin. There is evidence that renal fibrosis closely linked with chronic inflammatory cell infiltrate within interstitium, but potential role for mast cells this process has yet to be defined. Therefore, numbers normal kidneys various pathologies were investigated.Mast quantified transplants showing acute rejection cyclosporin toxicity, removed...