A5088032224- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- Genetics, Aging, and Longevity in Model Organisms
- RNA Interference and Gene Delivery
- Leptospirosis research and findings
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Microtubule and mitosis dynamics
- Telomeres, Telomerase, and Senescence
- Immune Response and Inflammation
- Radioactive contamination and transfer
- Neuroendocrine regulation and behavior
- Retinal Development and Disorders
- Carcinogens and Genotoxicity Assessment
- Nuclear and radioactivity studies
- Turtle Biology and Conservation
- Xenotransplantation and immune response
- 3D Printing in Biomedical Research
- Veterinary medicine and infectious diseases
- Advanced biosensing and bioanalysis techniques
- FOXO transcription factor regulation
- Chromosomal and Genetic Variations
- Vitamin C and Antioxidants Research
- Animal Virus Infections Studies
Tohoku University
2012-2019
Japan Society for the Promotion of Science
2015-2016
In this study, we created porcine-induced pluripotent stem (iPS) cells with the expression of six reprogramming factors (Oct3/4, Klf4, Sox2, c-Myc, Lin28, and Nanog). The resulting showed growth dependent on LIF (leukemia inhibitory factor) multiple cell markers. Furthermore, iPS caused teratoma formation three layers differentiation had both active X chromosomes (XaXa). Our satisfied important characteristics cells: activation chromosomes. Injection these into morula stage embryos that...
Nonhuman primates are useful animal models for the study of human diseases. However, number established cell lines from nonhuman is quite limited compared with other experimental animals. The establishment primate would allow drug testing on those before moving experiments into primates. In this study, we primary by introducing genes CDK4R24C, cyclin D1, and hTERT. These proliferated more rapidly than cells bypassed cellular senescence. Karyotype analysis showed that chromosome patterns were...
Prairie voles show strong pair bonding with their mating partners, and they demonstrate parental behavior toward infants, indicating that the prairie vole is a unique animal model for analysis of molecular mechanisms social behavior. Until recent study, signaling pathway oxytocin was thought to be critical voles, but neuron-specific functional research may necessary identify pluripotent stem cells high quality are essential elucidate behaviors. Generation high-quality induced (iPSCs) would...
Conservation of the genetic resources endangered animals is crucial for future generations. The loggerhead sea turtle (Caretta caretta) a critically species, because human hunting, hybridisation with other and infectious diseases. In present study, we established primary fibroblast cell lines from turtle, showed its species specific chromosome number 2n = 56, which identical to that hawksbill olive ridley turtles. We first intensive hybridization among multiple caused due number, allows...
The prairie vole (Microtus ochrogaster) shows social behaviors such as monogamy and parenting of infants with pair bonding. These are specific to the have not been observed in other types voles, mountain voles. Although has several unique characteristics, an vitro cell culture system established for this species. Furthermore, establishment cultured cells derived from may be beneficial based on three Rs (i.e., Replacement, Reduction, Refinement) concept. Therefore, study, we attempted...
Abstract Pigs throughout the world are afflicted with leptospirosis, causing serious economic losses and potential hazards to human health. Although it has been known that leptospiral lipopolysaccharide ( L‐LPS ) is involved in an immunological reaction between antigen a host cell, little about how immune system of pigs can respond . Here, we stimulated pig fibroblasts by then quantitatively measured gene protein expression levels two toll‐like receptors TLRs ), TLR2 TLR4 , real‐time PCR W...
Pigs are important, both for agriculture and as animal models human diseases. However, due to the lack of embryonic stem cells, possibility genetic modification is quite limited. To overcome this limitation, induced pluripotent (iPS) cells have been derived from pigs. Despite public availability a large number expression datasets mice, rats, primates-derived iPS profile pig-derived Furthermore, there no dataset focused on profiling cell with six reprogramming factors (Oct3/4, Sox2, Klf4,...
The 2011 earthquake severely damaged the Fukushima Daiichi Nuclear Power Plant (FNPP), resulting in release of large quantities radioactive material into environment. deposition these radionuclides rice straw as livestock feed led to circulation contaminated beef market. Based on safety concern consumers, a reliable method for estimating concentrations cesium muscle tissue is needed. In this study, we analyzed blood and skeletal 88 cattle, detected linear correlation between them. We then...
Abstract The introduction of four key transcriptional factors (CRX, RAX, NEURO‐D, OTX2) allows the direct differentiation fibroblasts to retinal photoreceptor cells. This reprogramming was achieved with a combination mono‐cistronic viruses. Although viruses useful, relatively high titer recombinant necessary because co‐infections are required. To overcome this issue, we established poly‐cistronic expression system for and analyzed biological characteristics introduced cells after exogenous...