A5063898024- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Crystallography and molecular interactions
- Organometallic Complex Synthesis and Catalysis
- Asymmetric Hydrogenation and Catalysis
- SARS-CoV-2 detection and testing
- Metalloenzymes and iron-sulfur proteins
- RNA and protein synthesis mechanisms
- Inorganic Chemistry and Materials
- Biosensors and Analytical Detection
- Organometallic Compounds Synthesis and Characterization
- Advanced biosensing and bioanalysis techniques
- Catalysis and Hydrodesulfurization Studies
- Fungal and yeast genetics research
- Electrocatalysts for Energy Conversion
- Metal complexes synthesis and properties
- CRISPR and Genetic Engineering
- Cyclopropane Reaction Mechanisms
- Dental Implant Techniques and Outcomes
- Catalytic Processes in Materials Science
- SARS-CoV-2 and COVID-19 Research
- Bone Tissue Engineering Materials
- Chemical Synthesis and Analysis
- Yersinia bacterium, plague, ectoparasites research
- Organophosphorus compounds synthesis
Boston University
2024
Heidelberg University
2014-2023
DKFZ-ZMBH Alliance
2017-2020
German Cancer Research Center
2020
Connecticut Children's Medical Center
2020
A. N. Nesmeyanov Institute of Organoelement Compounds
2019
Institute of General and Inorganic Chemistry
2019
Advisory Board Company (United States)
2019
Haldor Topsoe (Denmark)
2001-2015
Danish Geotechnical Society
2015
A colorimetric isothermal RNA amplification method was shown to detect SARS-CoV-2 in clinical samples with excellent sensitivity and specificity.
Here we describe a time-efficient strategy for endogenous C-terminal gene tagging in mammalian tissue culture cells. An online platform is used to design two long gene-specific oligonucleotides PCR with generic template cassettes create linear dsDNA donors, termed cassettes. encode the tag (e.g., GFP), Cas12a CRISPR RNA cleavage of target locus, and short homology arms directed integration via homologous recombination. The integrated coupled terminator shielding tagged from co-inserted...
A major factor governing the performance of catalytically active particles supported on a zeolite carrier is degree dispersion. It shown that introduction noncrystallographic mesopores into single crystals (silicalite-1, ZSM-5) may increase particle As representative examples, metal (Pt), an alloy (PtSn), and carbide (β-Mo2C) were conventional mesoporous carriers, respectively, dispersion was compared by TEM imaging. On zeolites, material aggregated outer surface particles, particularly...
Production of indigoidine can be enhanced by swapping a synthetic T domain into the NRPS IndC.
Abstract The COVID-19 pandemic caused by the novel SARS-CoV-2 virus poses a significant public-health problem. In order to control pandemic, rapid tests for detecting existing infections and assessing spread are critical. Approaches detect viral RNA based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) hold outstanding promise towards greatly simplified broadly applicable testing methods. RT-LAMP assays appear more robust than qPCR-based methods only require...
Abstract Throughout the SARS‐CoV‐2 pandemic, limited diagnostic capacities prevented sentinel testing, demonstrating need for novel testing infrastructures. Here, we describe setup of a cost‐effective platform that can be employed in high‐throughput manner, which allows surveillance as an acute pandemic control and preparedness tool, exemplified by diagnostics academic environment. The strategy involves self‐sampling based on gargling saline, pseudonymized sample handling, automated RNA...
Racemic dirhodium(II) complexes derived from orthometalated aryl phosphines, Rh2(O2CCH3)2(pc)2 (pc = phosphine, with head-to-tail arrangement) (1−7), are isolated as pure enantiomers by conventional resolution methods. They the first examples of chiral catalysts without ligands. These compounds have been used in cyclization α-diazo ketones; influence catalyst and substrate on enantioselectivity is studied. Results compared those obtained for reactions catalyzed Rh2(protos)4 Rh2(protos)2(pc)2...
The propensity of the terminal ruthenium carbide Ru(C)Cl2(PCy3)2 (RuC) to form bridges electron-rich transition metals enables synthetic routes metal clusters with coexisting and sulfide ligands. Electrochemical experiments show Ru≡C ligand exert a relatively large electron-withdrawing effect compared PPh3, effectively shifting redox potentials.
Chiral dirhodium(II) compounds, Rh2(O2CR)2(pc)2 (pc = ortho-metalated arylphosphine), with a head-to-tail arrangement (1−21) are used in the cyclopropanation of α-diazo ketones. The influence catalyst ligands and substrate structure on enantioselectivity is studied. Temperature solvent dependence assays performed, best ee values being obtained refluxing pentane. Results compare favorably those reported literature using Ru, Cu, other Rh(II) catalysts.
Exchange of the aqua ligands in [(H2O)9Mo3S4][pts]4·9H2O for Cp' (Cp' = methylcyclopentadienyl; pts p-toluenesulfonate) yielded novel cluster salt [(η5-Cp')3Mo3S4][pts] ([1][pts]). Reactions [1][pts] presence triphenylphosphane with [Ni(cod)2] (cod 1,5-cyclooctadiene) or [Pd2(dba)3] (dba dibenzylidenacetone) afforded heterobimetallic cubane-like compounds [(η5-Cp')3Mo3S4M'(PPh3)][pts] ([2][pts]: M' Ni; [3][pts]: Pd). By reaction [Pt(nor)3] (nor 2-norbornene) at ambient temperature...
Reaction between [(eta5-Cp')3Mo3S4]+ and [Ni(1,5-cod)2] (Cp' = methylcyclopentadienyl; 1,5-cod 1,5-cyclooctadiene) in THF at ambient temperature yielded a coordinatively unsaturated cubane-like cluster cation, [(eta5-Cp')3Mo3S4Ni]+. The ligand sphere the Ni atom could be saturated by coordinating dimethyl sulfide, diethyl di(tert-butyl) tetrahydrothiophene, thiochroman-4-ol, 1,4-dithiane, pyridine, quinoline, or 4,4'-bipyridine. products structurally model mode of substrate coordination on...
This work shows how a molecular Mo3S4 cluster bonded to photoelectrode surface via phosphonate ligand can be highly effective co-catalyst in photocathodic hydrogen evolution systems. Using TiO2 protected n+p Si photocathode, H2 occurs with an onset of +0.33 V vs. RHE acid solution this precious metal-free system. just the red part AM1.5 solar spectrum (λ > 635 nm), saturation current 20 mA/cm2 is achieved from electrode containing dropcasted onto 100 nm TiO2/7 Ti/n+p electrode.
By reaction of the geometrically incomplete cubane-like clusters [(eta(5)-Cp')(3)Mo(3)S(4))][pts] and [(eta(5)-Cp')(3)W(3)S(4)][pts] (Cp' = methylcyclopentadienyl; pts p-toluenesulfonate) with group 10 alkene complexes, three new heterobimetallic cluster cores were isolated: [(eta(5)-Cp')(3)W(3)S(4)M'(PPh(3))][pts] ([5][pts], M' Pd; [6][pts], Pt); [(eta(5)-Cp')(3)Mo(3)S(4)Ni(AsPh(3))][pts] ([7][pts]). The compounds [5][pts]-[7][pts] are completing extensive series...
Reaction of the methylcyclopentadienyl (Cp') cluster compound [(eta(5)-Cp')(3)Mo(3)S(4)][pts] (pts = p-toluenesulfonate) with noble metal alkene complexes resulted in formation four new heterobimetallic cubane-like Mo(3)S(4)M' cores (M' Ru, Os, Rh, Ir). Thus, reaction [(1,5-cod)Ru(CO)(3)] or [(1,3-cod)Os(CO)(3)] (cod cyclooctadiene) afforded [(eta(5)-Cp')(3)Mo(3)S(4)M'(CO)(2)][pts] Ru: [1][pts]; M' Os: [2][pts]). When [1][pts] was kept CH(2)Cl(2)/pentane solution, partial loss carbonyl...
Abstract Clone collections of modified strains (“libraries”) are a major resource for systematic studies with the yeast Saccharomyces cerevisiae . Construction such libraries is time-consuming, costly and confined to genetic background specific strain. To overcome these limitations, we present CRISPR-Cas12a (Cpf1)-assisted tag library engineering (CASTLING) multiplexed strain construction. CASTLING uses microarray-synthesized oligonucleotide pools in vitro recombineering program genomic...
The protein adsorption properties of the bioactive glass S53P4 were studied using albumin, IgG, and fibrinogen solutions (1 mg/mL) as well diluted plasma, serum, 1:1:1 mixtures fibrinogen. granules (315-500 microns) used in experiments without pretreatments or precorroded with an Si-rich a Ca,P-rich layer. compared to commercial (Bioglass), inert glass, experimental ceramic, titanium (Ti), hydroxyapatite (HA). untreated bound Trisbuffered saline mainly albumin from plasma serum mg each)...