A5008620000- Biotin and Related Studies
- Click Chemistry and Applications
- Amino Acid Enzymes and Metabolism
- Cellular transport and secretion
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Analytical Chemistry and Sensors
- Neurobiology and Insect Physiology Research
- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Genetics, Aging, and Longevity in Model Organisms
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Nanowire Synthesis and Applications
- Biosensors and Analytical Detection
- Invertebrate Immune Response Mechanisms
- Mitochondrial Function and Pathology
- Electrochemical sensors and biosensors
- Insect and Arachnid Ecology and Behavior
- FOXO transcription factor regulation
- Molecular Junctions and Nanostructures
- Wnt/β-catenin signaling in development and cancer
- Cell Image Analysis Techniques
- Neuroscience and Neural Engineering
- Retinoids in leukemia and cellular processes
Scripps Research Institute
2025
Scripps Institution of Oceanography
2021-2022
Scripps (United States)
2021-2022
Harvard University
2011-2021
Boston VA Research Institute
2021
Howard Hughes Medical Institute
2014
Brigham and Women's Hospital
2011
Harvard Stem Cell Institute
2011
Harvard–MIT Division of Health Sciences and Technology
2011
Massachusetts Institute of Technology
2011
Background The existence of neural stem and progenitor cells (together termed precursor cells) in the adult mammalian brain has sparked great interest utilizing these for regenerative medicine strategies. Endogenous precursors within forebrain subependyma can be activated following injury, resulting their proliferation migration toward lesion sites where they differentiate into cells. administration growth factors immunomodulatory agents injury augments this activation been shown to result...
We demonstrate the versatility of Al2O3-passivated Si nanowire devices ("nanoribbons") in analysis enzyme-substrate interactions via monitoring pH change. Our approach is shown to be effective through detection urea phosphate buffered saline (PBS), and penicillinase PBS urine, at limits <200 μM 0.02 units/mL, respectively. The ability extract accurate enzyme kinetics Michaelis-Menten constant (Km) from acetylcholine-acetylcholinesterase reaction also demonstrated.
Organ functions are highly specialized and interdependent. Secreted factors regulate organ development mediate homeostasis through serum trafficking inter-organ communication. Enzyme-catalysed proximity labelling enables the identification of proteins within a specific cellular compartment. Here, we report BirA*G3 mouse strain that CRE-dependent promiscuous biotinylation endoplasmic reticulum. When broadly activated throughout mouse, widespread was observed secretory pathway. Streptavidin...
Nature has evolved effective cell adhesion mechanisms to deliver inflammatory cells inflamed tissue; however, many culture-expanded therapeutic are incapable of targeting diseased tissues following systemic infusion, which represents a great challenge in therapy. Our aim was develop simple approaches program cell-cell interactions that would otherwise not exist toward and understanding the complex biology interactions. We employed chemistry approach engineer P- or L-selectin binding nucleic...
ABSTRACT Adipose tissue has varying distributions and metabolic properties between the sexes. Inherent sex‐specific differences in adipocytes may heighten risk of disease males. Analysis adipocyte proteome can potentially provide important insight. To enable cell‐type specific proteomic profiling vivo, we genetically engineered a mouse line for production promiscuous biotin ligase (BirA*G3) facilitating rapid isolation biotinylated proteomes. Adipocyte‐specific activation cytoplasmic BirA*G3...
Silicon nanowire field effect transistors (NWFETs) are low noise, power, ultrasensitive biosensors that highly amenable to integration. However, using NWFETs achieve direct protein detection in physiological buffers such as blood serum remains difficult due Debye screening, nonspecific binding, and stringent functionalization requirements. In this work, we performed an indirect sandwich immunoassay combined with exponential DNA amplification pH measurement by NWFET sensors. Measurements of...
Abstract Secreted interorgan communication factors encode key regulators of homeostasis. However, long-standing questions surround their origins/destinations, mechanisms interactions, and the number proteins involved. Progress has been hindered by lack methodologies for these factors’ large-scale identification characterization, as conventional approaches cannot identify low-abundance origins destinations secreted proteins. We established an in vivo platform to investigate protein...
Abstract Organ functions are highly specialized and interdependent. Secreted factors regulate organ development mediate homeostasis through serum trafficking inter-organ communication. Enzyme-catalyzed proximity labeling enables the identification of proteins within a specific cellular compartment. Here, we report BirA*G3 mouse strain that CRE-dependent promiscuous biotinylation endoplasmic reticulum. When broadly activated throughout mouse, widespread was observed secretory pathway....