A5101414386- Single-cell and spatial transcriptomics
- Click Chemistry and Applications
- Biotin and Related Studies
- Cancer Immunotherapy and Biomarkers
- Amino Acid Enzymes and Metabolism
- CAR-T cell therapy research
- Genomics and Phylogenetic Studies
- T-cell and B-cell Immunology
- Gut microbiota and health
- CRISPR and Genetic Engineering
- Fish Ecology and Management Studies
- Cellular transport and secretion
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Genetic diversity and population structure
- Immune cells in cancer
- Glycosylation and Glycoproteins Research
- Bat Biology and Ecology Studies
- Cancer Cells and Metastasis
- Fish Biology and Ecology Studies
- Plant Gene Expression Analysis
- Hydraulic Fracturing and Reservoir Analysis
- Microbial Natural Products and Biosynthesis
- Marine and coastal plant biology
Peking University
2021-2025
Center for Life Sciences
2022-2025
Peking University Third Hospital
2024
China University of Petroleum, East China
2024
BGI Group (China)
2018-2022
Novogene Bioinformatics Institute
2016-2019
Sichuan University
2016-2017
Tumor-infiltrating myeloid cells (TIMs) are key regulators in tumor progression, but the similarity and distinction of their fundamental properties across different tumors remain elusive. Here, by performing a pan-cancer analysis single from 210 patients 15 human cancer types, we identified distinct features TIMs types. Mast nasopharyngeal were found to be associated with better prognosis exhibited an anti-tumor phenotype high ratio TNF+/VEGFA+ cells. Systematic comparison between cDC1-...
T cells play a central role in cancer immunotherapy, but we lack systematic comparison of the heterogeneity and dynamics tumor-infiltrating across types. We built single-cell RNA-sequencing pan-cancer atlas for 316 donors 21 types revealed distinct cell composition patterns. found multiple state-transition paths exhaustion CD8+ preference those among different tumor Certain populations showed specific correlation with patient properties such as mutation burden, shedding light on possible...
Natural killer (NK) cells play indispensable roles in innate immune responses against tumor progression. To depict their phenotypic and functional diversities the microenvironment, we perform integrative single-cell RNA sequencing analyses on NK from 716 patients with cancer, covering 24 cancer types. We observed heterogeneity cell composition a tumor-type-specific manner. Notably, have identified group of tumor-associated that are enriched tumors, show impaired anti-tumor functions,...
Characterizing the compositional and phenotypic characteristics of tumor-infiltrating B cells (TIBs) is important for advancing our understanding their role in cancer development. Here, we establish a comprehensive resource human by integrating single-cell RNA sequencing data from 649 patients across 19 major types. We demonstrate substantial heterogeneity total abundance subtype composition observe immunoglobulin G (IgG)-skewness antibody-secreting cell isotypes. Moreover, identify...
Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we present the first report compare cheaper BGISEQ-500 platform Illumina HiSeq for scRNA-seq. We generate a resource of 468 single cells and 1297 matched cDNA samples, performing SMARTer Smart-seq2 protocols on two cell lines with RNA spike-ins. sequence these libraries both platforms using single- paired-end reads. The have comparable sensitivity accuracy in terms quantification gene expression, low...
Abstract Background The tumor microenvironment (TME) has been shown to strongly influence treatment outcome for cancer patients in various indications and the overall survival. However, cells forming TME gastric have not extensively characterized. Results We combine bulk single-cell RNA sequencing from tumors matched normal tissue of 24 treatment-naïve GC better understand which cell types transcriptional programs are associated with malignant transformation stomach. Clustering 96,623...
Emerging studies have focused on ways to treat cancers by modulating T cell activation. However, whether B receptor signaling in the tumor microenvironment (TME) can be harnessed for immunotherapy is unclear. Here, we report that an Asia-specific variant of human IgG1 containing a Gly396 Arg396 substitution (hIgG1-G396R) conferred improved survival patients with colorectal cancer (CRC). Mice knockin murine functional homolog mIgG2c-G400R recapitulated alleviated tumorigenesis and progression...
Organ functions are highly specialized and interdependent. Secreted factors regulate organ development mediate homeostasis through serum trafficking inter-organ communication. Enzyme-catalysed proximity labelling enables the identification of proteins within a specific cellular compartment. Here, we report BirA*G3 mouse strain that CRE-dependent promiscuous biotinylation endoplasmic reticulum. When broadly activated throughout mouse, widespread was observed secretory pathway. Streptavidin...
The genetic paradox of biological invasions is complex and multifaceted. In particular, the relative role disparate propagule sources adaptation through postintroduction hybridization has remained largely unexplored. To add resolution to this paradox, we investigate architecture responsible for invasion two invasive Asian carp species, bighead (
Programmed death 1 (PD-1) and the ligand of PD-1 (PD-L1) are central targets for immune-checkpoint therapy (ICT) blocking immune evasion-related pathways elicited by tumor cells. A number inhibitors have been developed, but efficacy these varies considerably is typically below 50%. The ICT has shown to be dependent on gut microbiota, experiments using mouse models even demonstrated that modulation microbiota may improve ICT.We followed a Han Chinese cohort 85 advanced non-small cell lung...
The Milu (Père David's deer, Elaphurus davidianus) were once widely distributed in the swamps (coastal areas to inland areas) of East Asia. dramatic recovery population is now deemed a classic example how highly endangered animal species can be rescued. However, molecular mechanisms that underpinned this remain largely unknown. Here, different approaches (genome sequencing, resequencing, and salinity analysis) utilized elucidate aforementioned mechanisms. comparative genomic analyses...
Abstract Seaweed is receiving an increasing amount of attention as a “sea vegetable”. The microbiota coastal populations may acquire seaweed associated enzymes through marine food. Several agarases have been found in non-marine environments; however, their origin unknown. In this study, hypothetical protein, Aga1, was identified agarase from inland soil agar-degrading bacterium, Paenibacillus sp. SSG-1.Having low similarity to known glycoside hydrolases, Aga1 be distant member the hydrolase...
Antibodies targeting the programmed cell death protein-1 (PD-1) molecule have been reported to hold promising antitumor activities in patients with nasopharyngeal carcinoma (NPC). However, only a small subset of NPC benefits from anti-PD-1 monotherapy and factors that affect treatment response need further investigation. This study aimed examine impact multiple genetic environmental on outcome immunotherapy by identifying tumor size, mutation burden (TMB) based whole exon sequencing, human...
Abstract The extensive environmental adaptability of the genus Paenibacillus is related to enormous diversity its gene repertoires. sp. SSG-1 has previously been reported, and agar-degradation trait attracted our attention. Here, genome sequence SSG-1, together with 76 sequenced strains, was comparatively studied. results show that pan-genome open indicate current taxonomy this incorrect. incessant flux repertoires resulting from processes gain loss largely contributed difference in genomic...
The Coptis chinensis Franch. is an important medicinal plant from the Ranunculales. We used next generation sequencing technology to determine complete chloroplast genome of C. chinensis. This 155,484 bp long with 38.17% GC content. Two 26,758 inverted repeats separated into a typical quadripartite structure. consists 128 gene loci, including eight rRNA 28 tRNA and 92 protein-coding loci. Most SSRs in are poly-A/T. numbers mononucleotide other Ranunculaceae species fewer than those...
Abstract The milu (Père David’s deer, Elaphurus davidianus ) has become a classic example of how highly endangered animal species can be rescued. However, the mechanisms that underpinned this population recovery remain largely unknown. As part study, we sequenced and analyzed whole genomes from multiple captive individuals. Following analysis, observed experienced prolonged decline over last 200,000 years, which led to an elongated history inbreeding. This protracted inbreeding facilitated...
Abstract All single-cell RNA-seq protocols and technologies require library preparation prior to sequencing on a platform such as Illumina. Here, we present the first report utilize BGISEQ-500 for scRNA-seq, compare sensitivity accuracy Illumina sequencing. We generate scRNA-seq resource of 468 unique single-cells 1,297 matched single cDNA samples, performing SMARTer Smart-seq2 mESCs K562 cells with RNA spike-ins. sequence these libraries both HiSeq platforms using single- paired-end reads....
Summary In seismic modeling and reverse time migration (RTM), it is essential to integrate anisotropy achieve precise wavefield produce high-quality images. The use of quasi-P-wave equations common in tilted transverse isotropic (TTI) media. However, traditional for the TTI media suffer from S-wave artifacts or require expensive complicated computational strategies. To tackle this issue, we introduce a novel pure equation with convolution operator Initially, break down conventional into an...