A5036219806- Medical Imaging Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Liver Disease Diagnosis and Treatment
- Prostate Cancer Treatment and Research
- Photoacoustic and Ultrasonic Imaging
- Cancer Cells and Metastasis
- Image Processing Techniques and Applications
- Cancer Genomics and Diagnostics
- Optical Imaging and Spectroscopy Techniques
- Cardiac Imaging and Diagnostics
- Molecular Biology Techniques and Applications
- Photodynamic Therapy Research Studies
- Prostate Cancer Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- AI in cancer detection
- Liver physiology and pathology
- Mitochondrial Function and Pathology
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Systemic Lupus Erythematosus Research
- Advanced MRI Techniques and Applications
- Urological Disorders and Treatments
- Radiomics and Machine Learning in Medical Imaging
- Insects and Parasite Interactions
- Image and Object Detection Techniques
Northwick Park Hospital
2008-2018
London North West Healthcare NHS Trust
2011-2016
St Mark's Hospital
2011-2016
Royal London Hospital
2011-2014
Imperial College London
2000-2014
Central Middlesex Hospital
2011-2014
Imperial College Healthcare NHS Trust
2011
Charing Cross Hospital
2003-2011
Northwest Hospital
2011
Cancer Research UK
2006
Human intestinal stem cell and crypt dynamics remain poorly characterized because transgenic lineage-tracing methods are impractical in humans. Here, we have circumvented this problem by quantitatively using somatic mtDNA mutations to trace clonal lineages. By analyzing imprints on the walls of colonic crypts, show that human cells conform one-dimensional neutral drift with a "functional" number five six both normal patients individuals familial adenomatous polyposis (germline APC−/+)....
The understanding of the fixation mutations within human tissues and their subsequent clonal expansion is a considerable problem, which little known. We have previously shown that nononcogenic in mitochondrial genome occur one number morphologically normal colonic crypt stem cells, progeny later occupy whole crypt. propose these wholly mutated crypts then clonally expand by fission, where each divides into two daughter crypts. Here we show ( i ) process fission share same genotype not...
Abstract We have used immunohistochemical and histochemical techniques to identify patches of hepatocytes deficient in the enzyme cytochrome c oxidase, a component electron transport chain encoded by mitochondrial DNA (mtDNA). These invariably abutted portal tracts expanded laterally as they spread toward hepatic veins. Here we investigate, using mtDNA mutations marker clonal expansion, clonality these patches. Negative were laser-capture microdissected identified polymerase reaction...
Abstract Fluorescence lifetime imaging (FLIM) depends on the fluorescence decay differences between tissues to generate image contrast. In present study FLIM has been applied fixed (but unstained) breast cancer demonstrate feasibility of this approach for histopathological assessment. As method relies natural autofluorescence, it may be possible circumvent tissue processing altogether and so potential a powerful new in vivo via an endoscopic or per‐operative variety organs, as well research...
After liver injury, parenchymal regeneration occurs through hepatocyte replication. However, during regenerative stress, oval cells (OCs) and small like progenitor (SHPCs) contribute to the process. We systematically studied intra-hepatic extra-hepatic sources of cell replacement in hepatitis B surface antigen (HBsAg-tg) mouse model chronic injury. Female HBsAg-tg mice received a bone marrow (BM) transplant from male HBsAg-negative mice, half these animals retrorsine block indigenous...
To evaluate the accuracy and biological basis for [(11)C]choline-PET-CT in nodal staging of high risk localized prostate cancer patients.Twenty-eight patients underwent dynamic pelvis lower abdomen prior to extended laparoscopic pelvic lymph node dissection (eLPL). The sensitivity specificity [(11)C]choline PET, PET-CT, MRI detection were calculated. Average maximal standardized uptake values (SUV(ave), SUV(max)) compared with choline kinase alpha (CHKα) Ki67 immunohistochemistry scores.Four...
Optical coherence tomography is capable of imaging the microstructures within tissues. To preserve transverse resolution at all depths, we implement a dynamic focusing scheme. improve quality images further, simple speckle reduction scheme employed which uses vibration introduced by translation stage used for axial scanning. A spatial compounding technique developed based on co-registration followed an averaging algorithm. We conclude that degree achieved worth expense more complicated...
We have applied fluorescence lifetime imaging (FLIM) to the autofluorescence of different kinds biological tissue in vitro, including animal sections and knee joints as well human teeth, obtaining two-dimensional maps with functional contrast. find that decay profiles are described by stretched exponential function (StrEF), which can represent complex nature tissue. The StrEF yields a continuous distribution lifetimes, be extracted an inverse Laplace transformation, additional information is...
Methods for lineage tracing of stem cell progeny in human tissues are currently not available. We describe a technique detecting the expansion single cell's that contain clonal mitochondrial DNA (mtDNA) mutations affecting expression mtDNA-encoded cytochrome c oxidase (COX). Because such take up to 40 years become phenotypically apparent, we believe these patches originate cells. Dual-color enzyme histochemistry was used identify COX-deficient cells, and were confirmed by microdissection...
Abstract Little is known about the clonal architecture of human urothelium. It likely that urothelial stem cells reside within basal epithelial layer, yet lineage tracing from a single cell as means to show presence has never been performed. Here, we identify clonally related areas bladder mucosa in order visualize fields maintained by founder/stem cell. Sixteen frozen cystectomy specimens were serially sectioned. Patches deficient for mitochondrially encoded enzyme cytochrome c oxidase...
A dual instrument is assembled to investigate the usefulness of optical coherence tomography (OCT) imaging in an ear, nose and throat (ENT) department. Instrument 1 dedicated vivo laryngeal investigation, based on endoscope probe head by compounding a miniature transversal flying spot scanning with commercial fiber bundle endoscope. This used implement channel nasolaryngeal endoscopy-OCT system. The two heads are provide simultaneously OCT cross section images en face endoscopic images. 2...
Screening Ziehl-Neelsen (ZN)-stained sections for acid-alcohol–fast bacilli (AAFB) is laborious, and sparse are easily missed. This article presents an automatic screening algorithm using digital image analysis designed to assist human diagnosis of tissue sections. The uses multiderivative source potentiators suppressors feeding into interconnected product nodes that result in a probability value each (the likelihood it contains AAFB) spatial map showing the position any bacillus. For study,...
Summary It is often desirable to perform digital image analyses on sections prepared for human interpretation, e.g. nuclear chromatin texture analysis or three‐dimensional reconstructions using requiring delineation of structures interest. Unfortunately such are more effective stains with less complex contrast. Here an automated selective ‘de‐staining’ method images presented. The separates into its red, green and blue hue, saturation intensity components. A mask stained tissue by automatic...
Cysts of the adrenal gland are rare and usually discovered incidentally. Large cysts can however present with severe abdominal pain be complicated by haemorrhage, rupture or infection. Adrenal pseudocysts appear to result from haemorrhage within a normal expand accommodate massive amounts fluid.We report case 39-year-old woman who presented worsening right upper quadrant pain. An ultrasound scan abdomen confirmed large 29 cm × 20 17 cyst that appeared originate in pole kidney causing...
It is widely accepted that the cell of origin breast cancer adult mammary epithelial stem cell; however, demonstrating presence and location tissue cells in human has proved difficult. Furthermore, we do not know clonal architecture normal premalignant epithelium or its cellular hierarchy. Here, use deficiency mitochondrial enzyme cytochrome c oxidase (CCO), typically caused by somatic mutations genome, as a means to perform lineage tracing epithelium. PCR sequencing laser-capture...
Summary This paper addresses the problem of 3D deconvolution through focus widefield microscope datasets ( Z ‐stacks). One most difficult stages in brightfield is finding point spread function. A theoretically calculated function (called a ‘synthetic PSF’ this paper) requires foreknowledge many system parameters and still gives only approximate results. measured from sub‐resolution bead suffers low signal‐to‐noise ratio, compounded setting (by contrast to fluorescence) by absorptive,...
Leishmaniasis is a disease that caused by protozoa of the genus Leishmania, which prevalent in tropical and subtropical areas. Clinical forms leishmaniasis are particularly diverse representing complex diseases. We present case lingual an immunocompetent man. The lesions were Leishmania donovani/infantum species. patient responded excellently to miltefosine treatment, with no reactivation during followup. To authors' knowledge, it first such successful treatment this unusual variant...