A5033997561- Genetic and Environmental Crop Studies
- Plant Pathogens and Fungal Diseases
- Agricultural pest management studies
- Plant Physiology and Cultivation Studies
- Fungal and yeast genetics research
- Botanical Research and Chemistry
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Advances in Cucurbitaceae Research
- Plant-Microbe Interactions and Immunity
- Postharvest Quality and Shelf Life Management
- Plant Virus Research Studies
- Cocoa and Sweet Potato Agronomy
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Photosynthetic Processes and Mechanisms
- Banana Cultivation and Research
- Horticultural and Viticultural Research
- Garlic and Onion Studies
- Plant Gene Expression Analysis
- Fermentation and Sensory Analysis
- Mycotoxins in Agriculture and Food
- Mangiferin and Mango Extracts
- Pomegranate: compositions and health benefits
Agricultural Research Organization
2016-2025
Hebrew University of Jerusalem
1992-2016
Institute for Systems Biology
2012
Ceragon (Israel)
2010
Genomics (United Kingdom)
2009
Seoul National University
2005
Massachusetts Institute of Technology
1995-1999
Whitehead Institute for Biomedical Research
1996-1999
Beth Israel Deaconess Medical Center
1999
Harvard University
1999
Summary Genome editing in plants has been boosted tremendously by the development of CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) technology. This powerful tool allows substantial improvement plant traits addition to those provided classical breeding. Here, we demonstrate virus resistance cucumber ( Cucumis sativus L.) using Cas9/subgenomic RNA (sgRNA) technology disrupt function recessive eIF4E eukaryotic translation initiation factor 4E ) gene. Cas9/sgRNA...
Overexpression of the Arabidopsis thaliana vacuolar H + -pyrophosphatase ( AVP1 ) confers salt tolerance to salt-sensitive ena1 mutant Saccharomyces cerevisiae . Suppression sensitivity requires two ion transporters, Gef1 Cl − channel and Nhx1 Na /H exchanger. These proteins colocalize prevacuolar compartment yeast are thought be required for optimal acidification this compartment. AtNHX1 , plant homologue exchanger, suppresses some phenotypes nhx1 mutant. Moreover, level mRNA in is...
Garlic is cultivated and consumed worldwide as a popular condiment green vegetable with medicinal neutraceutical properties. cultivars do not produce seeds, therefore, this plant has been the subject of either classical breeding or genetic studies. However, recent achievements in fertility restoration number genotypes have led to flowering seed production, thus enabling studies garlic.A transcriptome catalogue fertile garlic was produced from multiplexed gene libraries, using RNA collected...
Summary Furanocoumarins (FCs) are plant defence compounds derived from the phenylpropanoid pathway via coumarin umbelliferone that harbour some therapeutic benefits yet underlying cause of ‘grapefruit–drug interactions’ in humans. Most genes have not been identified citrus. We employed a genetic/Omics approach on citrus ancestral species and F1 populations mandarin × grapefruit pummelo. Enzyme specificity was characterized by In vivo 2‐oxoglutarate‐dependent dioxygenase family (2OGD)...
Colletotrichum gloeosporioides alkalinizes its surroundings during colonization of host tissue. The transcription factor pacC is a regulator pH-controlled genes and essential for successful colonization. We present here the sequence assembly fruit pathogen use it to explore global regulation pathogenicity by ambient pH. assembled genome size was 54 Mb, encoding 18,456 genes. Transcriptomes wild type ΔpacC mutant were established RNA-seq explored their pH-dependent gene regulation. analysis...
Penicillium expansum, the causal agent of blue mold rot, causes severe postharvest fruit maceration through secretion D-gluconic acid (GLA) and secondary metabolites such as mycotoxin patulin in colonized tissue. GLA involvement pathogenicity has been suggested but mechanism accumulation its contribution to P. expansum remain unclear. The roles were studied using i) glucose oxidase GOX2-RNAi mutants exhibiting decreased GOX2 expression, accumulation, reduced pathogenicity; ii) IDH-RNAi...
Pomegranate is a valuable crop that grown commercially in many parts of the world. Wild species have been reported from India, Turkmenistan and Socotra. fruit has variety health-beneficial qualities. However, despite this crop's importance, only moderate effort invested studying its biochemical or physiological properties establishing genomic genetic infrastructures. In study, we reconstructed transcriptome two phenotypically different accessions using 454-GS-FLX Titanium technology. These...
Mango (Mangifera indica) is an economically and nutritionally important tropical/subtropical tree fruit crop. Most of the current commercial cultivars are selections rather than products breeding programs. To improve efficiency mango breeding, molecular markers have been used to create a consensus genetic map that identifies all 20 linkage groups in seven mapping populations. Polyembryony trait, for clonal propagation rootstocks. In polyembryonic cultivars, addition zygotic embryo, several...
Summary P enicillium expansum , the causal agent of blue mould rot, causes severe post‐harvest fruit maceration simultaneously with secretion d ‐gluconic acid ( GLA ) and mycotoxin patulin in colonized tissue. The factor(s) inducing biosynthesis during colonization host acidic environment is unclear. During apple vivo growth culture, . secretes pH ‐modulating ammonia. Although its possible opportunistic precursor accumulate together fungal development, ammonia detected on tissue's leading...
Abstract Background Mango, Mangifera indica L., an important tropical fruit crop, is grown for its sweet and aromatic fruits. Past improvement of this species has predominantly relied on chance seedlings derived from over 1000 cultivars in the Indian sub-continent with a large variation size, yield, biotic abiotic stress resistance, quality among other traits. Historically, mango been orphan crop very limited molecular information. Only recently have genomics-based analyses enabled creation...
Quantitative traits are conditioned by several genetic determinants. Since such genes influence many important complex in various organisms, the identification of quantitative trait loci (QTLs) is major interest, but still encounters serious difficulties. We detected four linked within one QTL, which participate controlling sporulation efficiency Saccharomyces cerevisiae. Following single nucleotide polymorphisms comparing sequences 145 between parental strains SK1 and S288c, we analyzed...
Host-tissue alkalinization via ammonia accumulation is key to Colletotrichum spp. colonization. Using macroarrays carrying C. gloeosporioides cDNAs, we monitored gene expression during the process. A set of genes involved in synthesis and catabolism were identified. Expression NAD(+)-specific glutamate dehydrogenase (GDH2, encoding synthesis) exporter AMET induced at pH 4.0 4.5. Conversely, uptake transcript activation importers (MEP GLT, respectively) glutamine synthase (GS1) higher 6.0...
Tissue alkalinization during Colletotrichum gloeosporioides attack enhances the expression of PELB, which encodes pectate lyase (PL), and PL secretion, is considered essential for full virulence. We studied regulation secretion by manipulation C. PELB. PELB was down-regulated knocking out PAC1, PacC transcription factor that regulates gene products with pH-sensitive activities. functionally characterized a PACC homologue, from wild-type (WT) Cg-14 two independent deletion strains,...
Penicillium expansum, the causal agent of blue mold rot, causes severe postharvest maceration fruit through secretion total, d-gluconic acid (GLA). Two P. expansum glucose oxidase (GOX)-encoding genes, GOX1 and GOX2, were analyzed. GOX activity GLA accumulation strongly related to GOX2 expression, which increased with pH a maximum at 7.0, whereas was expressed 4.0, where no or extracellular detected. This differential expression also observed leading edge decaying tissue, dominant. The roles...
Commercial cultivars of garlic, a popular condiment, are sterile, making genetic studies and breeding this plant challenging. However, recent fertility restoration has enabled advanced physiological research hybridization in important crop. Morphophysiological studies, combined with transcriptome proteome analyses quantitative PCR validation, the identification genes specific processes involved gametogenesis fertile male-sterile garlic genotypes. Both genotypes exhibit normal meiosis at...