Pomegranate juice is well known for its health beneficial compounds, which can be attributed to high level of antioxidant activity and total polyphenol content. Our objective was study the relationships between activity, content, anthocyanins levels four major hydrolyzable tannins in different juices/homogenates prepared from sections fruit. To this end, 29 accessions were tested. The results showed that aril correlated significantly anthocyanin contents. However, homogenates whole fruit...

We report on the isolation, functional expression and characterization of a cDNA encoding chlorophyllase, enzyme catalyzing first step in chlorophyll breakdown pathway. The Chlase1 from Valencia Orange (Citrus sinensis cv. Valencia) was obtained by RT-PCR using degenerate primers based amino acid sequence previously purified protein. encodes protein 329 acids, including domain characterizing serine-lipases putative chloroplast-directing transit peptide. gene an active chlorophyllase which...

Abstract CesA genes are believed to encode the catalytic subunit of cellulose synthase. Identification nine distinctCesA cDNAs from maize (Zea mays) has allowed us initiate comparative studies with homologs Arabidopsis and other plant species. Mapping show that closely related not clustered but found at different chromosomal locations in both maize. Furthermore, sequence comparisons among CesA-deduced proteins these cluster groups wherein orthologs often more similar than paralogs,...

*Chilling requirement, together with heat determines the bloom date, which has an impact on climatic distribution of genotypes tree species. The molecular basis floral bud chilling requirement is poorly understood, despite its importance to adaptation and production fruit trees. In addition, genetic nature interrelationships among date remain unclear. *A peach (Prunus persica) F(2) population 378 developed from two contrasting requirements was used for linkage map construction quantitative...

Citrus fruits possess unique aromas rarely found in other fruit species. While flavor is composed of complex combinations soluble and volatile compounds, several low-abundance sesquiterpenes, such as valencene, nootkatone, alpha-sinensal, beta-sinensal, stand out citrus important aroma compounds. The profile terpenoid volatiles various species their importance compounds have been studied detail, but much still lacking our understanding the physiological, biochemical, genetic regulation...

The well-established health beneficial value of pomegranate juice is leading to increased demand for products and the expansion orchards worldwide. current study describes differences in chemical composition major ingredients arils peels 11 accessions grown Mediterranean desert climates Israel. In most accessions, levels antioxidant activity content total phenolics, anthocyanins, soluble solids, glucose, fructose, acidity were higher aril fruit climate compared those climate. However,...

Worldwide pomegranate (Punica granatum L.) production has expanded greatly due to recent evidence on the fruit health attributes. The fruit's unique red color, conferred by anthocyanins, is an imperative sensory quality. Climate effects internal color were reported earlier. present study investigated influence of a wide range temperature regimes (∼7–40 °C) pomegranates' aril anthocyanins. included two deciduous and evergreen accessions as well desert Mediterranean orchards. RP-HPLC analysis...

The current study describes differences in pomegranate fruit size and aril weight of 29 accessions grown Israel. contents sugars organic acids their juices peel homogenates, as well color parameters, were determined. While the levels total soluble solids (TSS) differ only slightly, those titratable acidity (TA) citric acid changed significantly, suggesting that they are main contributors to juice taste. In general, significant positive correlations found between TA values red these two TSS,...

Aqueous extracts of pomegranate peels were assayed in vitro for their antifungal activity against six rot fungi that cause fruit and vegetable decay during storage. The growth rates Alternaria alternata , Stemphylium botryosum Fusarium spp. significantly inhibited by the extracts. negatively correlated with levels total polyphenolic compounds extract particularly punicalagins, major ellagitannins peels. Ellagitannins also found to be main bioactive fractions using bioautograms, punicalagins...

Color is an important determinant of pomegranate fruit quality and commercial value. To understand the genetic factors controlling color in pomegranate, chemical, molecular characterization a "white" was performed. This unique accession lacking typical rendered by anthocyanins all tissues plant, including flowers, (skin arils) leaves. Steady-state gene-expression analysis indicated that none analyzed are able to synthesize mRNA corresponding PgLDOX gene (leucoanthocyanidin dioxygenase, also...

Pomegranate is a valuable crop that grown commercially in many parts of the world. Wild species have been reported from India, Turkmenistan and Socotra. fruit has variety health-beneficial qualities. However, despite this crop's importance, only moderate effort invested studying its biochemical or physiological properties establishing genomic genetic infrastructures. In study, we reconstructed transcriptome two phenotypically different accessions using 454-GS-FLX Titanium technology. These...

Apple (Malus domestica Borkh.) cultivars differ in their aroma and composition of volatile acetates fruit flesh peel. Cv. Fuji contains substantial levels 2-methyl butyl acetate (fruity banana-like odor), while the cv. Granny Smith apples lacks this compound. accumulate mainly hexyl (apple-pear odor) Feeding experiments indicated that were able to convert hexanol butanol respective derivatives vivo, could only exogenous acetate. Differential substrate specificities vitro acetyl-CoA:alcohol...

Commercial production of apricot is severely affected by sensitivity to climatic conditions, an adaptive feature essential for cycling between vegetative or floral growth and dormancy. Yield losses are due late winter early spring frosts inhibited caused unfulfilled chilling requirement (CR). Two cultivars, Perfection A.1740, were selected high low CR, respectively, develop a mapping population F 1 individuals using two-way pseudo-testcross strategy. High-density male female maps constructed...

A protein whose level is markedly increased upon exposure of cultured citrus cells and whole plants to NaCl, was shown specifically catalyze the reduction phosphatidylcholine hydroperoxide in presence glutathione. This enzymatic activity be independent a similar exhibited by glutathione S-transferase plants. finding corroborates significant homology (52%) accounted between deduced amino acid sequence gene encoding for this that mammalian phospholipid peroxidases. While enzyme known well...

Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role ability to confer resistance plants, we tried obtain transgenic overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts regenerated expressing this enzyme constitutively failed. However, when the enzyme's catalytic activity was abolished by active site-directed mutagenesis, inactive...