A5090059150- Genetic Mapping and Diversity in Plants and Animals
- Plant Disease Resistance and Genetics
- Genetics and Plant Breeding
- Plant and Fungal Interactions Research
- Plant Pathogens and Fungal Diseases
- Nuts composition and effects
- Cocoa and Sweet Potato Agronomy
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Plant Pathogens and Resistance
- Genetic and phenotypic traits in livestock
- Plant Pathogenic Bacteria Studies
- Plant Physiology and Cultivation Studies
- Chromosomal and Genetic Variations
- Wheat and Barley Genetics and Pathology
- Horticultural and Viticultural Research
- Plant-Microbe Interactions and Immunity
- Soybean genetics and cultivation
- Advances in Cucurbitaceae Research
- Legume Nitrogen Fixing Symbiosis
- Genetic diversity and population structure
- Plant nutrient uptake and metabolism
- Crop Yield and Soil Fertility
- Berry genetics and cultivation research
- Cassava research and cyanide
University of Tennessee at Knoxville
2018-2025
North Carolina State University
2012-2022
University of Cape Coast
2022
Plant (United States)
2016-2021
North Central State College
2014-2018
United States Department of Agriculture
2016
University of Wisconsin–Madison
2016
Agricultural Research Service
2016
University of Delaware
2016
Clemson University
2009-2012
Sweetpotato [Ipomoea batatas (L.) Lam.] is a globally important staple food crop, especially for sub-Saharan Africa. Agronomic improvement of sweetpotato has lagged behind other major crops due to lack genomic and genetic resources inherent challenges in breeding heterozygous, clonally propagated polyploid. Here, we report the genome sequences its two diploid relatives, I. trifida triloba, show that these high-quality assemblies are robust references hexaploid sweetpotato. Comparative...
The hexaploid sweetpotato (
β-Carotene content in sweetpotato is associated with the Orange and phytoene synthase genes; due to physical linkage of sucrose synthase, β-carotene starch are negatively correlated. In populations depending on for food security, an important source calories, while provitamin A. The negative association between two traits contributes low nutritional quality consumed, especially sub-Saharan Africa. Using a biparental mapping population 315 F
The Chinese chestnut (Castanea mollissima) carries resistance to Cryphonectria parasitica, the fungal pathogen inciting blight. pathogen, introduced from Asia, devastated American dentata) throughout its native range early in twentieth century. A highly informative genetic map of was constructed extend genomic studies Fagaceae and aid introgression blight genes into chestnut. Two mapping populations were established with three parents, 'Mahogany', 'Nanking', 'Vanuxem', totaling 337 progeny....
Corn borers are the primary maize pest; their feeding on pith results in stem damage and yield losses. In this study, we performed a genome-wide association study (GWAS) to identify SNPs associated with resistance Mediterranean corn borer diversity panel using set of more than 240,000 SNPs. Twenty five were significantly three traits: 10 tunnel length, 4 damage, 11 kernel resistance. Allelic variation at each significant SNP was from 6 9% phenotypic variance. A genes containing or physically...
Sweetpotato (Ipomoea batatas) plays a critical role in food security and is the most important root crop worldwide following potatoes cassava. In United States (US), it valued at over $700 million USD. There are two sweetpotato germplasm collections (Plant Genetic Resources Conservation Unit US Vegetable Laboratory) maintained by USDA, ARS for improvement. To date, no genome-wide assessment of genetic diversity within these has been reported published literature. our study, population...
The process of evolution under domestication has been studied using phylogenetics, population genetics–genomics, quantitative trait locus (QTL) mapping, gene expression assays, and archaeology. Here, we apply an evolutionary genetic approach to understand the constraints imposed by architecture variation in teosinte, wild ancestor maize, consequences on architecture. Using modern teosinte maize landrace populations as proxies for domesticate, respectively, estimated heritabilities, additive...
Much remains unknown of molecular events controlling the plant hypersensitive defense response (HR), a rapid localized cell death that limits pathogen spread and is mediated by resistance (R-) genes. Genetic control HR hard to quantify due its microscopic nature. Natural modifiers ectopic phenotype induced an aberrant auto-active R-gene (Rp1-D21), were mapped in population 3,381 recombinant inbred lines from maize nested association mapping population. Joint linkage analysis was conducted...
In developing countries, the sweetpotato, Ipomoea batatas (L.) Lam. [Formula: see text], is an important autopolyploid species, both socially and economically. However, quantitative trait loci (QTL) mapping has remained limited due to its genetic complexity. Current fixed-effect models can fit only a single QTL are generally hard interpret. Here, we report use of random-effect model approach map multiple based on score statistics in sweetpotato biparental population ('Beauregard' ×...
Commercial production of apricot is severely affected by sensitivity to climatic conditions, an adaptive feature essential for cycling between vegetative or floral growth and dormancy. Yield losses are due late winter early spring frosts inhibited caused unfulfilled chilling requirement (CR). Two cultivars, Perfection A.1740, were selected high low CR, respectively, develop a mapping population F 1 individuals using two-way pseudo-testcross strategy. High-density male female maps constructed...
In plants, most disease resistance genes encode nucleotide binding Leu-rich repeat (NLR) proteins that trigger a rapid localized cell death called hypersensitive response (HR) upon pathogen recognition. The maize (Zea mays) NLR protein Rp1-D21 derives from an intragenic recombination between two NLRs, Rp1-D and Rp1-dp2, confers autoactive HR in the absence of infection. From previous quantitative trait loci genome-wide association study, we identified single-nucleotide polymorphism locus...
Quantitative resistance to maize common rust (causal agent Puccinia sorghi) was assessed in an association mapping population of 274 diverse inbred lines. Resistance found be moderately correlated with three other diseases and the severity hypersensitive defense response previously same population. Using a mixed linear model accounting for confounding effects structure flowering time, genome-wide tests were performed based at 246,497 single-nucleotide polymorphism loci. Three loci associated...
Sweetpotato (Ipomoea batatas) is the sixth most important food crop and plays a critical role in maintaining security worldwide. Support for sweetpotato improvement research breeding genetics programs, maintenance of germplasm collections essential preserving future generations. Germplasm seek to preserve phenotypic genotypic diversity through accession characterization. However, due its genetic complexity, high heterogeneity, polyploid genome, plasticity, flower production variability,...
Rp1-D21 is a maize auto-active resistance gene conferring spontaneous hypersensitive response (HR) of variable severity depending on genetic background. We report an association mapping strategy based the Mutant Assisted Gene Identification and Characterization approach to identify naturally occurring allelic variants associated with phenotypic variation in HR. Each member collection 231 diverse inbred lines constituting high-resolution panel were crossed parental stock heterozygous for...
Continuous storage root formation and bulking (CSRFAB) in sweetpotato is an important trait from agronomic biological perspectives. Information about the molecular mechanisms underlying CSRFAB traits lacking.Here, as a first step toward understanding genetic basis of sweetpotato, we performed genome-wide association study (GWAS) using phenotypic data four distinct developmental stages 33,068 single nucleotide polymorphism (SNP) insertion-deletion (indel) markers. Based on Bonferroni...
The genetics of domestication has been extensively studied ever since the rediscovery Mendel's law inheritance and much learned about genetic control trait differences between crops their ancestors. Here, we ask how altered architecture by comparing 18 traits in maize its ancestor teosinte using matched populations. We observed a strongly reduced number QTL for relative to teosinte, which is consistent with previously reported depletion additive variance selection during domestication. also...
Albeit extensive cultivation of bitter melon both as vegetable and medicine in many countries Asia, Africa, South America, no serious efforts have been made for genetic breeding studies on this 'orphan' crop.In contrast to popular cucurbits, it lacks a linkage map required genomic depiction precise breeding.We report here the construction first using set 146 F2 progenies derived from an inter-botanical variety cross between Taiwan White, Momordica charantia var.charantia, CBM12, M....
Chinese chestnut (Castanea mollissima) has emerged as a model species for the Fagaceae family with extensive genomic resources including physical map, dense genetic map and quantitative trait loci (QTLs) blight resistance. These enable comparative genomics analyses relative to plants. We assessed degree of conservation between genome other well annotated assembled plant sequences, focusing on QTL regions most interest breeding community.The integrated been improved now include 858 shared...
Utilizing a high-density integrated genetic linkage map of hexaploid sweetpotato, we discovered major dominant QTL for root-knot nematode (RKN) resistance and modeled its effects. This discovery is useful development modern sweetpotato breeding program that utilizes marker-assisted selection genomic approaches faster gain RKN resistance. The [Meloidogyne incognita (Kofoid & White) Chitwood] causes significant storage root quality reduction yields losses in cultivated [Ipomoea batatas (L.)...
Global sweetpotato production is increasing due to its health benefits, including high levels of complex carbohydrates and bioactive compounds. To explore the genetic basis carotenoids, we conducted a genome-wide association study (GWAS) using diverse accessions, two decades phenotypic data, 252,975 dosage-based SNPs INDELs. Our findings confirmed negative correlation between dry matter β-carotene identified interconnected metabolic pathways regulating multiple traits. Notably, phytoene...
Three Chinese chestnut bacterial artificial chromosome (BAC) libraries were developed and used for physical map construction. Specifically, high information content fingerprinting was to assemble 126,445 BAC clones into 1,377 contigs 12,919 singletons. Integration of the dense genetic with achieved via high-throughput hybridization using overgo probes derived from sequence-based markers. A total 1,026 anchored including 831 corresponding 878 expressed sequence tag-based Within map, three...
Physiological leaf spotting, or flecking, is a mild-lesion phenotype observed on the leaves of several commonly used maize (Zea mays) inbred lines and has been anecdotally linked to enhanced broad-spectrum disease resistance. Flecking was assessed in nested association mapping (NAM) population, comprising 4,998 recombinant from 25 biparental families, an 279 diverse inbreds. Joint family linkage analysis conducted with 7,386 markers NAM population. Genome-wide tests were performed 26.5...
Abstract Blackberries (Rubus spp.) are the fourth most economically important berry crop worldwide. Genome assemblies and annotations have been developed for Rubus species in subgenus Idaeobatus, including black raspberry (R. occidentalis), red idaeus), R. chingii, but very few genomic resources exist blackberries their relatives Rubus. Here we present a chromosome-length assembly annotation of diploid blackberry germplasm accession “Hillquist” argutus). is only known source...
Abstract Next-generation sequencing (NGS) enables massively parallel acquisition of large-scale omics data; however, objective data quality filtering parameters are lacking. Although a useful metric, evidence reveals that platform-generated Phred values overestimate per-base scores. We have developed novel and empirically based algorithms streamline NGS filtering. The pipeline leverages known sequence motifs to enable empirical estimation error rates, detection erroneous base calls removal...