Pomegranate juice is well known for its health beneficial compounds, which can be attributed to high level of antioxidant activity and total polyphenol content. Our objective was study the relationships between activity, content, anthocyanins levels four major hydrolyzable tannins in different juices/homogenates prepared from sections fruit. To this end, 29 accessions were tested. The results showed that aril correlated significantly anthocyanin contents. However, homogenates whole fruit...

The well-established health beneficial value of pomegranate juice is leading to increased demand for products and the expansion orchards worldwide. current study describes differences in chemical composition major ingredients arils peels 11 accessions grown Mediterranean desert climates Israel. In most accessions, levels antioxidant activity content total phenolics, anthocyanins, soluble solids, glucose, fructose, acidity were higher aril fruit climate compared those climate. However,...

Collagen's biocompatibility, biodegradability and low immunogenicity render it advantageous for extensive application in pharmaceutical or biotechnological disciplines. However, typical collagen extraction from animal cadaver sources harbors risks including allergenicity potential sample contamination with pathogens. In this work, two human genes encoding recombinant heterotrimeric type I (rhCOL1) were successfully coexpressed tobacco plants the prolyl-4-hydroxylase (P4H) lysyl hydroxylase 3...

Abstract Cystathionine γ-synthase (CGS) is a key enzyme of Met biosynthesis in bacteria and plants. Aligning the amino acid sequences revealed that plant has an extended N-terminal region not found bacterial enzyme. However, this essential for catalytic activity enzyme, as deduced from complementation test Escherichia coli CGS mutant. To determine function region, we overexpressed full-length Arabidopsis its truncated version lacks transgenic tobacco (Nicotiana tabacum) Transgenic plants...

The current study describes differences in pomegranate fruit size and aril weight of 29 accessions grown Israel. contents sugars organic acids their juices peel homogenates, as well color parameters, were determined. While the levels total soluble solids (TSS) differ only slightly, those titratable acidity (TA) citric acid changed significantly, suggesting that they are main contributors to juice taste. In general, significant positive correlations found between TA values red these two TSS,...

Soybean seeds provide an excellent source of protein for human and livestock nutrition. However, their nutritional quality is hampered by a low concentration the essential sulfur amino acid, methionine (Met). In order to study factors that regulate Met synthesis in soybean seeds, this used Met-insensitive form Arabidopsis cystathionine γ-synthase (AtD-CGS), which first committed enzyme biosynthesis. This gene was expressed under control seed-specific promoter, legumin B4, transform cultivar...

Extracts from pomegranate (Punica granatum L.) organs are well known for their health beneficial properties that include antioxidative and cancer anti-proliferative activities. Polyphenols especially ellagitanins (ETs) were suggested as responsible these properties. In this study we measured the total phenol content (TPC); levels of several ETs, punicalagin, gallagic, ellagic gallic acids; activities water extracts different fruit sections tree organs. The ability to inhibit proliferation...

Aqueous extracts of pomegranate peels were assayed in vitro for their antifungal activity against six rot fungi that cause fruit and vegetable decay during storage. The growth rates Alternaria alternata , Stemphylium botryosum Fusarium spp. significantly inhibited by the extracts. negatively correlated with levels total polyphenolic compounds extract particularly punicalagins, major ellagitannins peels. Ellagitannins also found to be main bioactive fractions using bioautograms, punicalagins...

Abstract With an aim to elucidate novel metabolic and transcriptional interactions associated with methionine (Met) metabolism in seeds, we have produced transgenic Arabidopsis (Arabidopsis thaliana) seeds expressing a feedback-insensitive form of CYSTATHIONINE-γ-SYNTHASE, key enzyme Met synthesis. Metabolic profiling these revealed that, addition higher levels Met, the many other amino acids were elevated. The most pronounced changes stress-related (isoleucine, leucine, valine, proline),...

Abstract Pomegranate fruit peels are highly abundant in metabolites derived from the shikimate pathway, such as hydrolyzable tannins (HTs) and flavonoids. These beneficial to human health (commercial juice is enriched with peel metabolites), also protect environmental stresses. To understand transcriptional control of pathway-related pomegranate, we cloned characterized a subgroup S4 R2R3 Myb transcription factor, PgMyb308-like. Overexpressing PgMyb308-like pomegranate hairy roots increased...

Methionine is an essential amino acid in mammals and a precursor for vital metabolites required the survival of all organisms. Consequently, its inclusion diverse applications, such as food, feed, pharmaceuticals. Although acids other are commonly produced through microbial fermentation, high-yield biosynthesis L-methionine remains significant challenge due to strict cellular regulation pathway. As result, methionine primarily synthetically, resulting racemic mixture D,L-methionine. This...

With the aim of increasing methionine level in alfalfa (Medicago sativa L.) and thus improving its nutritional quality, we produced transgenic plants that expressed Arabidopsis cystathionine gamma-synthase (AtCGS), enzyme controls synthesis first intermediate metabolite pathway. The AtCGS cDNA was driven by rubisco small subunit promoter to obtain expression leaves. Thirty were examined for transgene protein expression, four lines with a high selected further work. In these lines, contents...

Summary Methionine and threonine are two essential amino acids, the levels of which limit nutritional quality plants. Both acids diverge from same branch aspartate family biosynthesis pathway; therefore, their pathways compete for carbon/amino substrate. To further elucidate regulation methionine seek ways increasing these we crossed transgenic tobacco plants overexpressing bacterial feedback‐insensitive kinase (bAK), containing a significantly higher level, with Arabidopsis cystathionine...

Plants have two kinds of fructokinases (FRKs) that catalyze the key step fructose phosphorylation, cytosolic and plastidic. The major tomato FRK, SlFRK2, is essential for development xylem vessels. In order to study role SlFRK3, which encodes only plastidic we generated transgenic (Solanum lycopersicon) plants with RNAi suppression SlFRK3 as well expressing beta-glucoronidase (GUS) under promoter. GUS staining indicated expression in vascular tissues leaves stems, including cambium,...

Summary Cystathionine γ ‐synthase (CGS), the first enzyme of methionine biosynthesis in higher plants, plays an important role pathway and regulating metabolism plant cells. In response to methionine, expression this is regulated via amino acid sequences located its N‐terminal. Here, using reverse transcription PCR ribonuclease protection analysis, we demonstrate that, addition full‐length CGS transcript, a deleted form exists Arabidopsis. The transcript that lacks 90 or 87 nt internally...