A5041196934- Extracellular vesicles in disease
- Immune cells in cancer
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Spondyloarthritis Studies and Treatments
- Proteoglycans and glycosaminoglycans research
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- Viral Infections and Immunology Research
- Atherosclerosis and Cardiovascular Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Autoimmune and Inflammatory Disorders Research
- Chemokine receptors and signaling
- Bone and Joint Diseases
- interferon and immune responses
- Nanopore and Nanochannel Transport Studies
- HIV Research and Treatment
- Cancer Immunotherapy and Biomarkers
- Phagocytosis and Immune Regulation
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Mesenchymal stem cell research
Jagiellonian University
2015-2024
Collegium Medicum in Bydgoszcz
2020
Atherosclerosis, a common age-related disease, is characterized by intense immunological activity. Atherosclerotic plaque composed of endothelial cells, vascular smooth muscle cells (VSMCs), lipids and immune infiltrating from the blood. During progression VSMCs undergo senescence within secrete SASP (senescence-associated secretory phenotype) factors that can actively modulate microenvironment. We demonstrated senescent increased number extracellular vesicles (senEVs). Based on unbiased...
Immune cells may take part in the renin-angiotensin-aldosterone system (RAAS), which plays a pivotal role regulation of vascular tone and blood pressure. The aim study was to analyse expression activity angiotensin-converting enzyme type 1 (ACE1) ACE2 human monocytes (MO) their subsets. highest relative level ACE1-, as well ACE2-mRNA expression, observed CD14(++)CD16(-) (classical) MO. Moreover, these cells, mean almost two times higher than that ACE1-mRNA (11.48 versus 7.073 units,...
Tumour-derived microvesicles (TMVs) are important players in tumour progression, modulating biological activity of immune cells e.g. lymphocytes, monocytes and macrophages. This phenomenon is particularly interesting the progression colon cancer, as macrophages this type relevant for recovery processes. In present study, role cancer cell-derived monocyte differentiation profile (polarization) was investigated. Monocyte-derived (MDM) were differentiated vitro presence TMVs obtained from...
Abstract Although blood monocytes exhibit significant cytotoxic activity against tumor cells, the function of infiltrating macrophages (TIM) is depressed in cancer patients. This study addresses question how antitumor response human monocytes, assessed by production cytokines (tumor necrosis factor α, TNF; IL-10; IL-12p40) and cytotoxicity, altered exposure to cells. Tumor cell−pre-exposed restimulated with cells showed significantly decreased TNF, IL-12, increased IL-10 (mRNA release)...
Cancer continues to be the leading cause of mortality in high-income countries, necessitating development more precise and effective treatment modalities. Immunotherapy, specifically adoptive cell transfer T receptor (TCR)-engineered cells (TCR-T therapy), has shown promise engaging immune system for cancer treatment. One biggest challenges TCR-T therapies is proper prediction pairing between TCRs peptide-human leukocyte antigen (pHLAs). Modern computational immunology, using artificial...
Tumour cells release membrane micro(nano)fragments called tumour-derived microvesicles (TMV) that are believed to play an important role in cancer progression. TMV suppress/modify antitumour response of the host, but there is also some evidence for their direct interaction with cells. In patients present body fluid and tumour microenvironment. The study aimed at characterization whole types/subpopulations, not only exosomes, from newly established gastric cell line (called GC1415) define...
Activation of neutrophils is an important mechanism in the pathology granulomatosis with polyangiitis (GPA). In this study, we evaluated whether extracellular vesicles (EVs) circulating plasma GPA patients could contribute to process. EVs from active stage disease (n = 10) and healthy controls were isolated by ultracentrifugation characterized flow cytometry (CD63, CD8) nanoparticle tracking analysis. Targeted oxylipin lipidomics was performed HPLC-MS/MS. EV/oxylipin-induced neutrophil traps...
Colon cancer constitutes 33% of all cases in humans and the majority patients with metastatic colon still have poor prognosis. An important role development is communication between normal cells. This may occur, among others, through extracellular vesicles (including microvesicles) (MVs), which are being released by both types MVs regulate a diverse range biological processes considered as useful biomarkers. Herein, we show that similarity general chemical composition cells their...
Advanced cystic fibrosis (CF) lung disease is commonly characterized by a chronic Pseudomonas aeruginosa infection and destructive inflammation caused neutrophils. However, the lack of convincing evidence from most informative biomarkers severe dysfunction (SLD-CF) has hampered formulation conclusive, targeted diagnosis CF. The aim this study was to determine whether SLD-CF related high concentration sputum inflammatory mediators presence biofilm-forming bacterial strains. Forty-one patients...
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Introduction. CD44H is a transmembrane molecule important for cell-cell and cell-extracellular matrix interactions. In monocytes, implicated in phagocytosis of particles coated by hyaluronan (HA). HA fragments were shown to induce chemokine secretion monocytes. Tumour derived microvesicles (TMVs), which are small membrane from tumour cells can carry HA. The aim the study was examine whether monocyte's involved engulfment pancreatic adenocarcinoma-derived production chemokines induced TMVs....