A5006300132- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Virus-based gene therapy research
- Influenza Virus Research Studies
- Microbial infections and disease research
- Veterinary medicine and infectious diseases
- Viral Infections and Immunology Research
- Animal Behavior and Welfare Studies
- Vector-Borne Animal Diseases
- Salmonella and Campylobacter epidemiology
- Animal Nutrition and Physiology
- Respiratory viral infections research
- Zoonotic diseases and public health
- Liver Disease and Transplantation
- Hepatitis B Virus Studies
- SARS-CoV-2 and COVID-19 Research
- Herpesvirus Infections and Treatments
- T-cell and Retrovirus Studies
- Agriculture and Farm Safety
- HIV Research and Treatment
- Effects of Environmental Stressors on Livestock
- Listeria monocytogenes in Food Safety
- Brucella: diagnosis, epidemiology, treatment
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2016-2025
Centre Hospitalier de Niort
2019-2025
CEA Paris-Saclay
2015-2024
Département Santé Animale
2024
Université Bretagne Loire
2016-2023
Inserm
2023
Centre de Recherche en Myologie
2023
Sorbonne Université
2023
University of Stuttgart
2022
Université Européenne de Bretagne
2013-2015
ABSTRACT Hepatitis E virus (HEV) infection of zoonotic origin is an emerging concern in industrialized countries. In the past few years, several cases hepatitis have been identified and consumption food products derived from pork liver associated with clusters human cases. More specifically, raw or undercooked incriminated. Few data on effect heating HEV inactivation are available. present study, various times temperatures that used during industrial processing were applied to experimentally...
In pig production, the widespread conventional indoor system with a slatted floor currently dominates. However, this production is becoming less socially acceptable. addition to general environmental protection issues, animal welfare, absence of suffering and distress, management pain also constitute societal concerns. context, alternative systems are gaining ground. Although they popular consumers other citizens, these have their critical points. Here, we reviewed international scientific...
Background. On 11 December 2013, 3 clustered cases of hepatitis E were reported on a French coastal island. Individuals had taken part in wedding meal that included spit-roasted piglet. The piglet been stuffed with raw stuffing partly made from the liver. Investigations carried out to identify vehicle contamination and evaluate dispersion virus (HEV) environment. Methods. A questionnaire was administered 98 participants who asked give blood sample. Cases identified by reverse...
Porcine circovirus type 2 (PCV2) is recognized as a primary cause in post-weaning multisystemic wasting syndrome (PMWS). In this study, both PCV1 and PCV2 types were studied pigs originating from PMWS-affected (+) non-affected (−) herds Brittany. was identified by PCR 100 % of animals PMWS(+) 76 PMWS(−) herds, while not detected. The complete sequences 38 isolates determined 23 new variants identified, displaying between 94·6 99·9 nucleotide identity with one another. Although highly related...
Frequent zoonotic transmission of hepatitis E virus (HEV) has been suspected, but data supporting the animal origin autochthonous cases are still sparse. We assessed genetic identity HEV strains found in humans and swine during an 18-month period France. sequences identified patients with infection (n = 106) were compared amplified from livers collected slaughterhouses 43). Phylogenetic analysis showed same proportions subtypes 3f (73.8%), 3c (13.4%), 3e (4.7%) human populations....
Concomitant infections by different influenza A virus subtypes within pig farms increase the risk of new reassortant emergence. The aims this study were to characterize epidemiology recurrent swine and identify their main determinants. follow-up was carried out in 3 selected known be affected repeated infections. Three batches pigs followed each farm from birth slaughter through a representative sample 40 piglets per batch. Piglets monitored individually on monthly basis for serology...
We report the first and only case of a porcine epidemic diarrhea (PED) outbreak occurring in December 2014 northern France, we show using full-length genome sequence French PED virus (PEDV) isolate that it was PEDV indel strain close to German strains recently isolated.
Hepatitis E virus (HEV) can cause enterically-transmitted hepatitis in humans. The zoonotic nature of infections has been established industrialized areas and domestic pigs are considered as the main reservoir. dynamics transmission pig herds therefore needs to be understood reduce prevalence viremic at slaughter prevent contaminated products from entering food chain. An experimental trial was carried out study characteristics HEV between orally inoculated naïve animals. A mathematical model...
In Europe, modified live vaccines (MLV) are commonly used to control porcine reproductive and respiratory syndrome virus (PRRSV) infection. However, they have been associated with safety issues such as reversion virulence induced by mutation and/or recombination. On a French pig farm, we identified field recombinant strain derived from two PRRSV-1 MLV (MLV1). As result, aimed evaluate its clinical, virological, transmission parameters in comparison both parental strains. Three groups six...
A Multiplex PCR-based assay (m-PCR) with three sets of primers was developed for the detection all serotypes Salmonella enterica and identification Enteritidis Typhimurium. This method evaluated against a bacteriological analysis environmental swabs poultry houses. Samples were pre-enriched in phosphate-buffered peptone water 24 h subjected to different protocols prior PCR: (i) an immunomagnetic separation using DynabeadsTM anti-Salmonella (Dynal); (ii) DNA extraction procedure InstageneTM...
A multiplex-PCR-based assay (m-PCR) was developed for the detection of Salmonella and identification two serotypes Enteritidis Typhimurium. Three sets primers selected from different genomic sequences amplified a 429 bp fragment specific genus within randomly cloned sequence, 559 target Typhimurium fliC gene 312 sefA gene. The m-PCR-based used detecting 1078 environmental swabs poultry houses. Prior to PCR, these were pre-enriched in phosphate-buffered peptone water 18–20 h then sub-cultured...
A survey of anelloviruses in swine herds from Britanny, France, is reported. By using PCR targeted to the conserved untranslated region, prevalences 93 and 73 % were found among 15 33 animals, respectively. The lung was organ be positive most frequently five organs tested 32 animals. highest identity levels our nucleotide sequences with pig isolates Japan an isolate Tupaia belangeri. Interestingly, when aligning all available France Japan, at least two phylogenetic groups identified, each...