A5100783904- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Machine Learning in Bioinformatics
- Gene Regulatory Network Analysis
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Genetic and phenotypic traits in livestock
- RNA Research and Splicing
- Sperm and Testicular Function
- Congenital heart defects research
- Mathematical and Theoretical Epidemiology and Ecology Models
- Pulmonary Hypertension Research and Treatments
- Renal cell carcinoma treatment
- Crystallization and Solubility Studies
- Genomics and Chromatin Dynamics
- Cancer, Hypoxia, and Metabolism
- RNA and protein synthesis mechanisms
- Cardiac Fibrosis and Remodeling
- Cardiac Valve Diseases and Treatments
- Amyotrophic Lateral Sclerosis Research
- Reproductive Biology and Fertility
Nanning Normal University
2019-2024
Shandong University
2013-2024
The University of Texas Southwestern Medical Center
1996-2024
Southwestern Medical Center
2021-2023
China Agricultural University
2021-2023
North University of China
2022
Shanxi University
2022
ShenZhen People’s Hospital
2004-2022
Inner Mongolia Medical University
2022
Northeast Forestry University
2005-2021
Rationale: Cilp1 (cartilage intermediate layer protein 1) is a secreted extracellular matrix normally associated with bone and cartilage development. Its function mechanism of action in adult heart disease remain elusive. Objective: To establish the postmyocardial infarction (MI) cardiac remodeling. Methods Results: We investigated expression mouse models pathological remodeling human failure patients. was expressed predominantly fibroblasts upregulated response to injury blood patients...
Abstract Three‐dimensional modeling of the complex between retinoic acid‐binding protein (CRABP) and acid suggests thatbinding ligand is mediated by interaction carboxyl groupof two charged amino acids (Arg‐111 Arg‐131) whose side chains project into barrel protein. To assess contribution these to protein–ligand interaction, substitutions were made oligonucleotide‐directed, site‐specific mutagenesis. The wild‐type mutant proteins expressed in E. coli subsequently purified. Like CRABP, are...
Abstract The native state fluorescence and CD spectra of the predominantly β‐sheet cellular retinoic acid‐binding protein I (CRABPI) include contributions from its three tryptophan residues are influenced by positions these in three‐dimensional structure. Using a combination spectroscopic approaches single Trp‐mutants CRABPI, we have deconvoluted uncovered several features that aided our analysis development structure folding pathway CRABPI. emission spectrum CRABPI is dominated Trp 7. 109...
Abstract Background The incidence of congenital heart disease (CHD) is continuously increasing among infants born alive nowadays, making it one the leading causes infant morbidity worldwide. Various studies suggest that both genetic and environmental factors lead to CHD, therefore identifying its candidate genes disease-markers has been central topics in CHD research. By using high-throughput genomic data which are available recently, network-based methods provide powerful alternatives...
ZSM-5 zeolites with different mesoporosities were prepared by alkaline treatment and characterized powder XRD nitrogen adsorption. Two C7 hydrocarbons of n-heptane toluene employed as probe molecules to investigate the effects introduction mesopore on adsorption diffusion properties comparing experimental results samples treated untreated using NaOH. Adsorption isotherms measured gravimetrically in pressure range 0–32 mbar from 293 338 K. The microporous mesoporous successfully fitted...
Currently, the diagnosis of gastrointestinal diseases relies heavily on electronic endoscopy, which is not suitable for all individuals and insufficient dynamic patient monitoring. Therefore, there an urgent need a convenient noninvasive examination method to enhance monitoring diseases. Since tongue significant reflection digestive system closely associated with diseases, our study aims develop diagnostic framework based features. We have collected dataset 2,167 coating images from 949...
Abstract Integrating genomics and histology for cancer prognosis demonstrates promise. Here, we develop a multi-classifier system integrating lncRNA-based classifier, deep learning whole-slide-image-based clinicopathological classifier to accurately predict post-surgery localized (stage I–III) papillary renal cell carcinoma (pRCC) recurrence. The significantly higher predictive accuracy recurrence-free survival (RFS) compared the three single classifiers alone in training set both validation...
Sickness behaviors, including anorexia, are evolutionarily conserved responses to acute infections. Inflammation-induced anorexia causes dramatic metabolic changes, of which components critical survival unique depending on the type inflammation. Glucose supplementation during anorectic period induced by bacterial inflammation suppresses adaptive fasting pathways, fibroblast growth factor 21 (FGF21), and decreases survival. Consistent with this observation, FGF21-deficient mice more...
Abstract The mechanisms of neuronal degeneration in motor neuron disease are not fully understood. We tested the hypothesis that oxidative stress vitro and axotomy vivo induce single‐strand breaks (SSB) DNA, a form early DNA damage, adult neurons during their degeneration. developed characterized novel cell suspension system enriched from rat spinal cord ventral horn. This is ∼84% neurons, with ∼86% these being neurons; ∼72% α‐motor neurons. Motor viability ∼100% immediately after isolation...
Abstract Deciphering spatial domains using spatially resolved transcriptomics (SRT) is of great value for the characterizing and understanding tissue architecture. However, inherent heterogeneity varying resolutions present challenges in joint analysis multi-modal SRT data. We introduce a geometric deep learning method, named stMMR, to effectively integrate gene expression, location histological information accurate identifying from stMMR uses graph convolutional networks (GCN)...
Amyotrophic lateral sclerosis is a fatal disease caused by degeneration of motor neurons (MNs). We transplanted multipotent neural precursor cell (NPC)-neurospheres from mouse olfactory bulb (OB) into the spinal cord transgenic mice that develop MN because human mutant superoxide dismutase-1 (mSOD1). Adult NPCs were isolated OB core expressing green fluorescent protein, wild-type SOD1, or mSOD1. mSOD1 received lumbar transplants OB-NPC neurospheres at preclinical stages (70 days old)....
To test whether melatonin plays an important role in the process of early pregnancy, was given drinking water to pregnant mice at different gestation stages. These included who were 14 days prior their successful mating (confirmed by vaginal plug) (Group A), after B), and until C). Melatonin administration significantly enhanced serum as well ovarian levels mice. It observed that did not affect natural estrous On day 0.5 (D0.5), only elevated progesterone (P) secretion, but also upregulated...
Abstract Advances in spatial transcriptomics (ST) technologies have provided unprecedented opportunities to depict transcriptomic and histological landscapes the context. Multi-modal ST data provide abundant comprehensive information about cellular status, function, organization. However, dealing with processing analysis of data, existing algorithms struggle effectively fuse multi-modal contained within data. Here, we propose a graph contrastive learning-based cross-modality fusion model...
With rapid accumulation of functional relationships between biological molecules, knowledge-based networks have been constructed and stocked in many databases. These provide curated comprehensive information for linkages among genes proteins, whereas their activities are highly related with specific phenotypes conditions. To evaluate a network condition, the consistency its structure conditionally gene expression profiling data an important criterion. In this study, authors propose Gaussian...
Abstract Background Proteases are important for hydrolysis of proteins to generate peptides with many bioactivities. Thus, the development novel proteases high activities is meaningful discover bioactive peptides. Because natural isolation from animal, plant and microbial sources impractical produce large quantities proteases, gene cloning expression target protease preferred. Results In this study, an alkaline serine (GsProS8) Geobacillus stearothermophilus was successfully cloned expressed...
The androgen receptor (AR) plays a central role in prostate cancer. Development of castration resistant cancer (CRPC) requires androgen-independent activation AR, which involves its large N-terminal domain (NTD) and entails extensive epigenetic changes depending part on histone lysine demethylases (KDMs) that interact with AR. AR-NTD is rich low-complexity sequences, including polyQ repeat. Longer sequences were reported to decrease transcriptional activity protect against cancer, although...
An efficient photoredox‐catalyzed ring‐opening hydrogenolysis of spirocyclopropyl oxindoles by using diisopropylethylamine as reducing agent has been developed. Varieties 3‐substituted oxindole derivatives were achieved in moderate to good yields under mild reaction conditions. Furthermore, the synthetic utility is demonstrated further transformations synthesized products.
Abstract Background Fecundity is important for farm blue fox ( Vulpes lagopus ), who with asthenospermia have be a problem in some of farms China. A key symptom decreased sperm motility. The secreting beta-defensin108 vBD108 ) speculated related to asthenospermia. To clarify this idea, the mRNA expression testis and epididymis foxes were detected compared healthy one. antibody was prepared analyzed by immunohistochemistry. Results found both group method testes P < 0.05) epididymal corpus...
Background More and more high‐throughput datasets are available from multiple levels of measuring gene regulations. The reverse engineering regulatory networks these data offers a valuable research paradigm to decipher mechanisms. So far, numerous methods have been developed for reconstructing networks. Results In this paper, we provide review bioinformatics inferring network omics data. To achieve the precision reconstruction networks, an intuitive alternative is integrate resources in...