A5033466287- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Immunodeficiency and Autoimmune Disorders
- Mycobacterium research and diagnosis
- Immune Cell Function and Interaction
- Immune cells in cancer
- Tuberculosis Research and Epidemiology
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Computational Drug Discovery Methods
- SARS-CoV-2 and COVID-19 Research
- Cytokine Signaling Pathways and Interactions
- Long-Term Effects of COVID-19
- Protein Structure and Dynamics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- HIV Research and Treatment
- SARS-CoV-2 detection and testing
- Viral Infections and Outbreaks Research
- Prion Diseases and Protein Misfolding
- Machine Learning in Bioinformatics
- Curcumin's Biomedical Applications
- Crystallography and molecular interactions
- Digestive system and related health
- Immune responses and vaccinations
- Myasthenia Gravis and Thymoma
National Institute of Allergy and Infectious Diseases
2012-2025
National Institutes of Health
2012-2025
National Institute on Aging
2020-2025
SRM Institute of Science and Technology
2023
Indian Institute of Science Bangalore
2018-2023
Walter Reed Army Institute of Research
2022
Henry M. Jackson Foundation
2022
Institute on Aging
2022
Institute of Aging
2021
University of Maryland, College Park
2016-2020
Abstract Background Cytotoxic T-lymphocyte-associated protein 4 deficiency (CTLA4-D) is an inborn error of immunity (IEI) caused by heterozygous mutations, and characterized immune cell infiltration into the gut other organs, leading to intestinal disease, dysregulation autoimmunity. While regulatory T-cell dysfunction remains central CTLA4-D immunopathogenesis, mechanisms driving disease severity pathology are unknown but likely involve dysbiosis. We determined whether microbiome metabolome...
ABSTRACT Complement activation has long been associated with inflammation, primarily due to the elaboration of complement anaphylotoxins C5a and C3a. In this work, we demonstrate that phagocytosis complement-opsonized particles promotes host inflammatory responses by a new mechanism depends on terminal components (C5b–C9). We during particles, membrane attack complex (MAC) can be transferred from activating particle macrophage plasma ‘bystander’ mechanism. This MAC-mediated bystander damage...
Vaccination protects against severe COVID-19 manifestations. For those with post-COVID-19 conditions (PCC) or long COVID, the impact of vaccination on evolution symptoms, immune responses, and viral persistence is unclear.In this prospective observational cohort study, we evaluated number PCC affected organ systems, psychological well-being scores before after patients received vaccination. We simultaneously biomarkers systemic inflammation levels plasma cytokines/chemokines. measured...
Dietary interventions with bioactive compounds have been found to suppress the accumulation of senescent cells and senescence-associated secretory phenotypes (SASPs). One such compound, curcumin (CUR), has beneficial health biological effects, including antioxidant anti-inflammatory properties, but its ability prevent hepatic cellular senescence is unclear. The objective this study was investigate effects dietary CUR as an on determine benefits aged mice. We screened transcriptome that...
Abstract Macrophages readily change their phenotype in response to exogenous stimuli. In this work, macrophages were stimulated under a variety of experimental conditions, and phenotypic alterations correlated with changes gene expression. We identified 3 transcriptionally related populations immunoregulatory activity. They generated by stimulating cells TLR ligands the presence different “reprogramming” signals: high-density ICs, PGE2, or Ado. All these cell produced high levels transcripts...
Chronic granulomatous disease (CGD) is caused by defects in any 1 of the 6 subunits forming nicotinamide adenine dinucleotide phosphate oxidase complex 2 (NOX2), leading to severely reduced or absent phagocyte-derived reactive oxygen species production. Almost 50% patients with CGD have inflammatory bowel (CGD-IBD). While conventional IBD therapies can treat CGD-IBD, their benefits must be weighed against risk infection. Understanding impact NOX2 on intestinal microbiota may lead...
Abstract Few live attenuated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines are in pre-clinical or clinical development. We seek to attenuate SARS-CoV-2 (isolate WA1/2020) by removing the polybasic insert within spike protein and open reading frames (ORFs) 6–8, introducing mutations that abolish non-structural 1 (Nsp1)-mediated toxicity. The derived virus (WA1-ΔPRRA-ΔORF6-8-Nsp1 K164A/H165A ) replicates 100- 1000-fold-lower titers than ancestral induces little lung...
Chronic granulomatous disease (CGD) is an inborn error of immunity caused by defects in any one the 6 subunits (gp91phox, p47phox, p22phox, p67phox, p40phox or chaperone EROS) forming nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex 2 (NOX2) and resulting defective phagocyte-derived reactive oxygen species (ROS) production. Almost 50% patients with CGD have inflammatory bowel (IBD) associated dysbiosis age IBD onset may vary according to genotype. While we previously...
Handling cultured isolates and clinical, environmental, or wildlife surveillance samples containing Risk Group 3 4 pathogens presents considerable biosafety challenges in minimizing human exposure during processing transport. Safe handling typically requires high- maximum-containment facilities, demanding substantial logistical planning resources. We evaluated PrimeStore Molecular Transport Medium (PS-MTM), a guanidine-based solution created to kill preserve nucleic acids at ambient...
Abstract Diversity and plasticity are the hallmarks of macrophages. The two most well-defined macrophage subsets classically activated macrophages (CAMϕs) IL-4–derived alternatively (AAMϕs). Through a series studies, we previously identified characterized distinct population with immunoregulatory functions, collectively termed regulatory (RMϕs). Although considerable advances have been made in understanding these various subsets, it is not known whether one activation state can influence...
Abstract Cannabinoid 1 receptor (CB1R) expression is upregulated in the liver with viral hepatitis, cirrhosis, and both alcoholic non-alcoholic fatty disease (FLD), whereas its muted under usual physiological conditions. Inhibiting CB1R has been shown to be beneficial preserving hepatic function FLD but it unclear if inhibiting during an inflammatory response acute injury, such as toxin-induced would also beneficial. We found that intrinsic hepatocytes regulated inflammation-related gene...
Toll-like receptors (TLRs) that recognize pathogen associated molecular patterns and chemoattractant (CKRs) orchestrate leukocyte migration to infected tissue are two arms of host innate immunity. Although TLR signaling induces synthesis secretion proinflammatory cytokines chemokines, which recruit leukocytes, many studies have reported the paradoxical observation stimulation inhibits chemotaxis in vitro impairs their recruitment tissues during sepsis. There is consensus physical loss...
Beta-2 microglobulin (β2m) is an amyloidogenic protein belonging to the immunoglobulin superfamily, responsible for dialysis-related amyloidosis (DRA).
Background Mycobacterium abscessus subspecies massiliense (M. massiliense) is increasingly recognized as an emerging bacterial pathogen, particularly in cystic fibrosis (CF) patients and CF centres' respiratory outbreaks. We characterized genomic phenotypic changes 15 serial isolates from two (1S 2B) with chronic pulmonary M. infection leading to death, well four a centre outbreak which patient 2B was the index case.Results Comparative analysis revealed mutations affecting growth rate,...
Human and Simian Immunodeficiency virus (HIV-1, HIV-2, SIV) encode an accessory protein, Nef, which is a pathogenesis virulence factor. Nef multivalent adapter that dysregulates the trafficking of many immune cell receptors, including chemokine receptors (CKRs). Physiological endocytic itinerary agonist occupied CXCR4 involves ubiquitinylation phosphorylated receptor at three critical lysine residues dynamin-dependent through ESCRT pathway into lysosomes for degradation. Likewise, induced...