Regulation and dysregulation of intracellular calcium (Ca2+) signaling via the inositol 1,4,5-trisphosphate receptor (InsP3R) has been linked to many cellular processes pathological conditions. In present study, addition neuronal sensor-1 (NCS-1), a high-affinity, low-capacity, calcium-binding protein, purified InsP3R type 1 (InsP3R1) increased channel activity in both calcium-dependent -independent manner. intact cells, enhanced expression NCS-1 resulted release upon stimulation...

Leptospira spp., the causative agents of leptospirosis, adhere to components extracellular matrix, a pivotal role for colonization host tissues during infection. Previously, we and others have shown that immunoglobulin-like proteins (Lig) spp. bind fibronectin, laminin, collagen, fibrinogen. In this study, report can be immobilized by human tropoelastin (HTE) or elastin from different tissues, including lung, skin, blood vessels, Lig HTE elastin. Moreover, both same LigB domains, LigBCon4,...

Leptospira spp. are pathogenic spirochetes that cause the zoonotic disease leptospirosis. Leptospiral immunoglobulin (Ig)-like protein B (LigB) contributes to binding of extracellular matrix proteins such as fibronectin, fibrinogen, laminin, elastin, tropoelastin and collagen. A high-affinity Fn-binding region LigB has been localized LigBCen2, which contains partial 11th full 12th Ig-like repeats (LigBCen2R) 47 amino acids non-repeat (LigBCen2NR) LigB. In this study, gelatin domain...

Summary Transposon insertions in a novel 3.798 kb open reading frame (ORF) of the rice pathogen, Xanthomonas oryzae pv. ( Xoo ) cause virulence deficiency and altered colony/lawn morphology. This ORF encodes protein, XadA, 1265 amino acids that exhibits significant similarity to non‐fimbrial adhesins animal pathogenic bacteria such as Yersinia YadA Moraxella UspA1. An interesting feature is region repeated six times within XadA sequence encompasses almost entire length protein. Anti‐XadA...

The β- and γ-crystallins are closely related lens proteins that members of the βγ-crystallin superfamily, which also include many non-lens members. Although β-crystallin is known to be a calcium-binding protein, this property has not been reported in γ-crystallin. We have studied calcium binding properties γ-crystallin, we show it binds 4 mol eq with dissociation constant 90 µm. It calcium-mimic spectral probes, terbium Stains-all. Calcium does significantly influence protein secondary...

Pathogenic Leptospira spp. express immunoglobulin-like proteins, LigA and LigB, which serve as adhesins to bind extracellular matrices mediate their attachment on host cells. However, nothing is known about the mechanism by these proteins are involved in pathogenesis. We demonstrate that LigBCen2 binds Ca2+, evidenced inductively coupled plasma optical emission spectrometry, energy dispersive 45Ca overlay, mass although there no motif for Ca2+ binding. four determined matrix-assisted laser...

Abnormal levels of endogenous calcium ions are known to induce eye lens opacity, and a variety causative factors has been proposed, including calcium-mediated aggregation precipitation the proteins crystallins. We have specifically looked in some detail at interaction Ca2+ with various crystallins its consequences. Lenses incubated solutions containing 10 mM or 5 Tb3+ opacified. Fluorescence titration TbCl3 revealed that this ion binds delta- beta-crystallins solution. Equilibrium dialysis...

Phosphatidylinositol 4-OH kinase IIIbeta (PI-4Kbeta) is involved in the regulated local synthesis of phospholipids that are crucial for trans-Golgi network (TGN)-to-plasma membrane trafficking. In this study, we show calcium sensor proteins calneuron-1 and calneuron-2 physically associate with PI-4Kbeta, inhibit enzyme profoundly at resting low levels, negatively interfere Golgi-to-plasma At high levels inhibition released PI-4Kbeta activated via a preferential association neuronal sensor-1...

The βγ-crystallin superfamily consists of evolutionarily related proteins with domain topology similar to lens β- and γ-crystallins, formed from duplicated Greek key motifs. Ca2+ binding was found in a few members earlier, although its prevalence diversity as inherent molecular properties among the are not well studied. To increase our understanding various βγ-crystallins, we undertook comprehensive structural Ca2+-binding studies seven bacteria, archaea, vertebrates, including determination...

Crystallins are the major proteins of a mammalian eye lens. The topologically similar lens proteins, beta- and gamma-crystallins, prototype founding members betagamma-crystallin superfamily. Betagamma-crystallins have until recently been regarded as structural proteins. However, calcium-binding properties few potential role betagamma-crystallins in fertility being investigated. Because elements other member such spherulin 3a, not present betaB2-crystallin from fish genomes, it was argued...

Leptospira immunoglobulin-like (Lig) proteins including LigA and LigB are adhesins that bind to fibronectin, collagen, laminin elastin. In addition, Lig fibrinogen (Fg)-binding proteins, although the physiological role of Lig-Fg interaction is unclear. this study, a previously identified Fg-binding region, LigBCen2 (amino acids 1014-1165 LigB), has been further localized LigBCen2R, which consists partial 11th entire 12th Ig-like domain 1014-1119). LigBCen2R was found C-terminal αC Fg (FgαCC;...

purpose. Previous studies have identified sequences encoding vascular endothelial growth factor (VEGF)-A and one of the VEGF receptors (VEGFR2, Flk-1, KDR) in lens fiber cells. The current study was undertaken to determine distribution VEGF-A protein lens, whether signaling through occurs cells, pattern expression during development, effect hypoxia on expression. methods. VEGFR2 were localized using immunocytochemistry. quantified by Western blot analysis. Activated (tyrosine phosphorylated)...

Background Many bacterial surface exposed proteins mediate the host-pathogen interaction more effectively in presence of Ca2+. Leptospiral immunoglobulin-like (Lig) proteins, LigA and LigB, are containing Bacterial immunoglobulin like (Big) domains. The function which contain Big fold is not known. Based on possible similarities βγ-crystallin folds, we here explore important question whether Ca2+ binds to a domains, would provide novel functional role fold. Principal Findings We selected six...

To understand the disease mechanism and to identify potential tumor markers that would help in therapeutics, comparative proteomic analysis of 29 retinoblastoma (RB) tumors was performed using 14 non-neoplastic retinas (age ranged from 45 89 years) as control tissues.2-DE MALDI-TOF-TOF MS/MS were used differentially expressed proteins.Twenty-seven distinct proteins identified, including 16 upregulated 11 downregulated proteins. Significantly, higher mRNA levels apolipoprotein A1 (p<0.001),...

Neuronal calcium sensor-1 (NCS-1), a Ca2+-binding protein, plays an important role in the modulation of neurotransmitter release and phosphatidylinositol signaling pathway. It is known that physiological activity NCS-1 governed by its myristoylation. Here, we present myristoylation NSC-1 governing Ca2+ binding Ca2+-induced conformational changes as compared with nonmyristoylated protein. The 45Ca isothermal titration calorimetric data show increases degree cooperativity; thus, myristoylated...