A5036364679- Ocular Oncology and Treatments
- Cancer-related Molecular Pathways
- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Brain Metastases and Treatment
- Retinal and Optic Conditions
- Ocular Diseases and Behçet’s Syndrome
- Nonmelanoma Skin Cancer Studies
- Glioma Diagnosis and Treatment
- Retinal Imaging and Analysis
- interferon and immune responses
- Ocular Infections and Treatments
- Glaucoma and retinal disorders
- Retinal and Macular Surgery
- Cutaneous Melanoma Detection and Management
- Head and Neck Surgical Oncology
- MicroRNA in disease regulation
- Corneal Surgery and Treatments
- Advanced biosensing and bioanalysis techniques
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Ocular Disorders and Treatments
- RNA Interference and Gene Delivery
- Intraocular Surgery and Lenses
- CNS Lymphoma Diagnosis and Treatment
University of Rochester
2023-2025
Sankara Nethralaya
2015-2024
University of Toronto
2024
Princess Margaret Cancer Centre
2024
Golisano Children's Hospital
2023
Creative Commons
2022-2023
All India Institute of Medical Sciences
2023
RTD Services (Austria)
2021
Aditya Birla (India)
2020
Vasan Eye Care Hospital
2020
Retinoblastoma clinical observations revealed the role of tumor suppressor genes in human cancer, Knudson's 'two-hit' model cancer induction. We now demonstrate that loss both RB1 gene alleles initiates quiescent RB1−/− retinomas with low level genomic instability and high expression senescence-associated proteins p16INK4a p130. Although can remain unchanged throughout life, highly proliferative, clonal aneuploid retinoblastomas commonly emerge, exhibiting altered copy number oncogenes...
To prospectively determine the prevalence of high-risk histopathologic features (HRFs) in patients with unilateral retinoblastoma who undergo enucleation and to evaluate role chemotherapy preventing recurrences.Children newly diagnosed enucleated were enrolled prospectively. After central histopathology review, specific HRFs received chemotherapy; others observed. Primary end points event-free survivals (EFS).Of 331 during 2005 2010, 321 eligible had review. Discordance between review...
Abstract Background Epithelial cell adhesion molecule (EpCAM) is overexpressed in solid tumors and regarded as a putative cancer stem marker. Here, we report that employing EpCAM aptamer (EpApt) siRNA (SiEp) dual approach, for the targeted delivery of to positive cells, efficiently inhibits proliferation. Results Targeted using polyethyleneimine one efficient methods gene delivery, thus, developed novel aptamer-PEI-siRNA nanocomplex targeting. PEI synthesized with showed 198 nm diameter...
PurposeTo compare metastasis-related mortality, local treatment failure, and globe salvage after retinoblastoma in countries with different national income levels.DesignInternational, multicenter, registry-based retrospective case series.ParticipantsTwo thousand one hundred ninety patients, 18 ophthalmic oncology centers, 13 on 6 continents.MethodsMulticenter data were pooled from patients enrolled between January 2001 December 2013. Adequate to allow American Joint Committee Cancer staging,...
To evaluate the ability of 8th edition American Joint Committee on Cancer (AJCC) Staging Manual to estimate metastatic and mortality rates for children with retinoblastoma (RB).International, multicenter, registry-based retrospective case series.A total 2190 patients from 18 ophthalmic oncology centers 13 countries over 6 continents.Patient-specific data fields RB were designed selected by subcommittee. All adequate records allow tumor staging AJCC criteria follow-up disease...
To evaluate the ability of American Joint Committee on Cancer (AJCC) 8th edition to predict local tumor control and globe salvage for children with retinoblastoma (RB).International, multicenter, registry-based retrospective case series.A total 2854 eyes 2097 patients from 18 ophthalmic oncology centers 13 countries over 6 continents.International, data were pooled enrolled between January 2001 December 2013. All RB adequate records allow staging by AJCC criteria follow-up ascertain...
Retinoblastoma is the most common pediatric ocular malignancy. It triggered by a biallelic mutation in RB1 gene or MYCN oncogene amplification. Retinoblastomas can be unilateral (60%-70%) bilateral (30%-40%); tumors are always heritable and present at an earlier age as compared to ones (18-24 months vs. 36 India). High prevalence rates, delayed presentation, inaccessibility healthcare lead worse outcomes developing countries. The past few decades have seen paradigm change treatment of...
Retinoblastoma (RB) is a malignant tumor of the retina seen in children, and potential non invasive biomarkers are need for rapid diagnosis prognosticating therapy. This study was undertaken to identify differentially expressed miRNAs serum children with RB comparison normal age matched serum, analyze its concurrence existing miRNA profile, novel gene targets specific RB, expression few identified oncogenic advanced stage primary patient's sample. MiRNA profiling performed on 14 pooled from...
Epithelial cell adhesion molecule (EpCAM), a cancer stem (CSC) marker is over expressed in epithelial cancers and retinoblastoma (RB). We fabricated an EpCAM targeting aptamer-siRNA chimera investigated its anti-tumor property intracellular domain (EpICD) mediated signaling cancer. The efficacy of aptamer-siEpCAM (EpApt-siEp) was evaluated by qPCR, northern Western blotting WERI-Rb1- RB line, primary tumor cells MCF7- breast line. Anti-tumor activity EpApt-siEp studied vivo using (MCF7) mice...
Retinoblastoma (RB), a primary pediatric intraocular tumor, arises from primitive retinal layers. Several novel molecular strategies are being developed for the clinical management of RB. miRNAs known to regulate cancer-relevant biological processes. Here, role selected miRNAs, namely, miR-532-5p and miR-486-3p, has been analyzed potential therapeutic targeting in RB.A comprehensive bioinformatic analysis was performed predict posttranscriptional regulators (miRNAs) select panel genes [Group...
To correlate the differentially expressed miRNAs with clinico-pathological features in uveal melanoma (UM) tumors harbouring chromosomal 3 aberrations among South Asian Indian cohort.Based on aberration, UM (n = 86) were grouped into monosomy (M3; n 51) and disomy (D3; 35) by chromogenic in-situ hybridisation (CISH). The recorded. miRNA profiling was performed formalin fixed paraffin embedded (FFPE) samples 6) using Agilent, Human microarray, 8x15KV3 arrays. association between studied...
AIM: To determine which IIRC scheme was used by retinoblastoma centers worldwide and the percentage of D eyes treated primarily with enucleation versus globe salvaging therapies as well to correlate trends in treatment choice version geographic region. METHODS: An anonymized electronic survey offered 115 physicians at 39 asking about classification schemes patterns between 2008 2012. Participants were asked record for classification, how many group diagnosed, therapies. Averages per modality...
Retinoblastoma is a rare pediatric tumor of the retina, caused by homozygous loss 1 (RB1) suppressor gene. Previous microarray studies have identified changes in expression profiles coding genes, however our understanding how non-coding genes change this tumor, absent. This an important area research, as many adult malignancies, including LNC-RNAs are used biomarkers to predict outcome and/or relapse. To establish complete, and in-depth RNA profile, both tumors, we conducted RNA-seq from...
Abstract Local intravitreal or intra-arterial chemotherapy has improved therapeutic success for the pediatric cancer retinoblastoma (RB), but toxicity remains a major caveat. RB initiates primarily with RB1 loss or, rarely, MYCN amplification, critical downstream networks are incompletely understood. We set out to uncover perturbed molecular hubs, identify synergistic drug combinations target these vulnerabilities, and expose overcome resistance. applied dynamic transcriptomic analysis...
To determine the value of clinical features for advanced intraocular retinoblastoma as defined by eighth edition American Joint Committee on Cancer (AJCC) cT3 category and AJCC Ophthalmic Oncology Task Force (OOTF) Size Groups to predict high-risk pathologic features.International, multicenter, registry-based retrospective case series.Eighteen ophthalmic oncology centers from 13 countries over 6 continents shared evaluations 942 eyes enucleated primary treatment and, comparison, cT2...
KIF14 a mitotic kinesin gene plays an important role in cytokinesis. Deregulation of may be pathway tumor progression and results decreased patient survival as seen breast tumors. Recently, KIF14, possible that drives gain chromosome arm 1q (the most commonly gained chromosomal region retinoblastoma), has been shown to strong oncogene candidate overexpressed by more than two orders magnitude retinoblastoma. This study was conducted quantify the expression human retinoblastoma tumors...
To understand the disease mechanism and to identify potential tumor markers that would help in therapeutics, comparative proteomic analysis of 29 retinoblastoma (RB) tumors was performed using 14 non-neoplastic retinas (age ranged from 45 89 years) as control tissues.2-DE MALDI-TOF-TOF MS/MS were used differentially expressed proteins.Twenty-seven distinct proteins identified, including 16 upregulated 11 downregulated proteins. Significantly, higher mRNA levels apolipoprotein A1 (p<0.001),...