A5025518725- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Amino Acid Enzymes and Metabolism
- Polyamine Metabolism and Applications
- Autophagy in Disease and Therapy
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- ATP Synthase and ATPases Research
- Enzyme function and inhibition
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Renal cell carcinoma treatment
- Cancer-related Molecular Pathways
- Pancreatic function and diabetes
- Cancer Research and Treatments
- Metabolism, Diabetes, and Cancer
- Renal Diseases and Glomerulopathies
- Renal and related cancers
- MicroRNA in disease regulation
- Genetic and Kidney Cyst Diseases
- Hippo pathway signaling and YAP/TAZ
- Glioma Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
- interferon and immune responses
Inserm
2016-2025
Université Côte d'Azur
2014-2025
Centre National de la Recherche Scientifique
2014-2024
Centre Méditerranéen de Médecine Moléculaire
2019-2024
Délégation Provence et Corse
2024
La Ligue Contre le Cancer
2023-2024
Institut de Chimie de Nice
2024
Observatoire de la Côte d’Azur
2024
Centre Antoine Lacassagne
2010-2022
Institut de Recherche sur le Cancer et le Vieillissement de Nice
2009-2021
While hypoxia-inducible factor (HIF) is a major actor in the cell survival response to hypoxia, HIF also associated with death. Several studies implicate HIF-induced putative BH3-only proapoptotic genes bnip3 and bnip3l hypoxia-mediated We, like others, do not support this assertion. Here, we clearly demonstrate that hypoxic microenvironment contributes rather than death by inducing autophagy. The ablation of Beclin1, autophagy, enhances under conditions. In addition, BNIP3 and/or BNIP3L...
Abstract Acidosis of the tumor microenvironment is typical a malignant phenotype, particularly in hypoxic tumors. All cells express multiple isoforms carbonic anhydrase (CA), enzymes catalyzing reversible hydration carbon dioxide into bicarbonate and protons. Tumor membrane-bound CAIX CAXII that are controlled via hypoxia-inducible factor (HIF). Despite recognition expression HIF-1α correlates with poor patient survival, role growth not fully resolved. To understand advantage derive from...
The resistance of hypoxic cells to radiotherapy and chemotherapy is a major problem in the treatment cancer. Recently, an additional mode hypoxia-inducible factor (HIF)-dependent transcriptional regulation, involving modulation specific set micro RNAs (miRNAs), including miR-210, has emerged. We have recently shown that HIF-1 induction miR-210 also stabilizes through positive regulatory loop. Therefore, we hypothesized by stabilizing normoxia, may protect cancer from radiation. developed...
Abstract The function of the hypoxia-inducible factor-1 (HIF-1), key transcription factor involved in cellular adaptation to hypoxia, is restricted low oxygen tension (pO2). As such, this central modulating tumor microenvironment, sensing nutrient availability, and controlling anaerobic glycolysis, intracellular pH, cell survival. Degradation inhibition limiting HIF-1α subunit are intimately connected normoxia. Hydroxylation two proline residues by prolyl hydroxylase domain (PHD) 2 protein...
The hypoxia-inducible factor 1 (HIF-1), in addition to genetic and epigenetic changes, is largely responsible for alterations cell metabolism hypoxic tumor cells. This transcription not only favors proliferation through the metabolic shift from oxidative phosphorylation glycolysis lactic acid production but also stimulates nutrient supply by mediating adaptive survival mechanisms. In this study we showed that glycogen synthesis enhanced non-cancer cancer cells when exposed hypoxia, resulting...
Abstract Hypoxia is an essential component of tumor microenvironment. In this study, we investigated the influence hypoxia (1% PO2) on CTL-mediated cell lysis. We demonstrate that exposure target cells to has inhibitory effect CTL clone (Heu171)-induced autologous Such inhibition correlates with hypoxia-inducible factor-1α (HIF-1α) induction but not associated alteration reactivity as revealed by granzyme B polarization or morphological change. Western blot analysis indicates although had no...
Carbonic anhydrase IX (CAIX) is an enzyme upregulated by hypoxia during tumour development and progression. This study was conducted to assess if the expression of CAIX in tissue and/or plasma can be a prognostic factor patients with non-small cell lung cancer (NSCLC). Tissue microarrays containing 555 NSCLC samples were generated for quantification expression. The level determined ELISA 209 these 58 healthy individuals. immunostaining levels correlated clinicopathological factors patient...
Metastatic renal cell carcinomas (mRCC) are highly vascularized tumors that a paradigm for the treatment with antiangiogenesis drugs targeting vascular endothelial growth factor (VEGF) pathway. The available increase time to progression but not curative and patients eventually relapse. In this study we have focused our attention on molecular mechanisms leading resistance sunitinib, first line of mRCC. Because anarchic vascularization core mRCC receives only suboptimal concentrations drug. To...
Metformin and IACS‐010759 are two distinct antimetabolic agents. Metformin, an established antidiabetic drug, mildly inhibits mitochondrial complex I, while is a new potent I inhibitor. Mitochondria pivotal in the energy metabolism of cells by providing adenosine triphosphate through oxidative phosphorylation (OXPHOS). Hence, OXPHOS become vulnerability when targeted cancer cells. Both drugs have promising antitumoral effects diverse cancers, supported preclinical vitro vivo studies. We...
Normal and tumor cells subjected to a hypoxic microenvironment show evidence of autophagy. We hypothesize that will sense hypoxia as warning signal upcoming drastic microenvironmental conditions autophagy, acting survival mechanism, provide time for adapt. This work demonstrates the first atypical BH3-domain BNIP3 BNIP3L, two HIF-target genes, can compete with Beclin 1-Bcl-2 1-Bcl-X(L) complexes, releasing 1 from complex then enhancing thus revealed new role BH3-only proteins in cellular...
Abstract It is well established that cells exposed to the limiting oxygen microenvironment (hypoxia) of tumors acquire resistance chemotherapy, through mechanisms not fully understood. We noted a large number cell lines showed protection from apoptotic stimuli, staurosporine, or etoposide, when long‐term hypoxia (72 h). In addition, these had unusual enlarged mitochondria were induced in HIF‐1‐dependent manner. Enlarged functional as they conserved their transmembrane potential and ATP...
Alternative Splicing of Goat aS1-Casein Transcripts nization the cUSl-CnA and cUSl-CnF alleles in region containing putatively out-spliced exons.In this report, we show that most transcripts from asl-CnF allele are actually aberrantly spliced, lack three exons.However, properly spliced messengers also produced, as well which up to five exons missing.Nine different were isolated characterized.Our results suggest a single base deletion within first unspliced exon insertions occurring...
OBJECTIVE Recent evidence indicates that low oxygen tension (pO2) or hypoxia controls the differentiation of several cell types during development. Variations pO2 are mediated through hypoxia-inducible factor (HIF), a crucial mediator adaptative response cells to hypoxia. The aim this study was investigate role in β-cell differentiation. RESEARCH DESIGN AND METHODS We analyzed capacity rat embryonic pancreas using two vitro assays. Pancreata were cultured either collagen on filter at...