A5089547252- Venomous Animal Envenomation and Studies
- Antimicrobial Peptides and Activities
- Ion channel regulation and function
- Insect and Pesticide Research
- Cell Adhesion Molecules Research
- Research on Leishmaniasis Studies
- RNA Research and Splicing
- Nicotinic Acetylcholine Receptors Study
- Cancer-related gene regulation
- Seaweed-derived Bioactive Compounds
- Viral Infectious Diseases and Gene Expression in Insects
- Insect Resistance and Genetics
- Cardiac electrophysiology and arrhythmias
- Natural product bioactivities and synthesis
- Cancer Mechanisms and Therapy
- Biochemical and Structural Characterization
- Cell death mechanisms and regulation
- Synthesis and biological activity
- Genomics, phytochemicals, and oxidative stress
- Medicinal Plant Pharmacodynamics Research
- Silk-based biomaterials and applications
- Cancer-related molecular mechanisms research
- Chromatography in Natural Products
- Garlic and Onion Studies
- Chemical synthesis and pharmacological studies
Tunis University
2015-2024
Tunis El Manar University
2015-2024
Institut Pasteur de Tunis
2010-2024
Physiopathologie et Epidémiologie des Maladies Respiratoires
2018
Centre de Biologie du Développement
2009
La Ligue Contre le Cancer
2007
In this study, we investigated for the first time anti-inflammatory and immunomodulatory properties of crude polysaccharide (PSHT) extracted from green marine algae Halimeda tuna. PSHT exhibited anti-oxidant activity in vitro through scavenging 1, 1-diphenyl-2-picryl hydroxyl free radical, reducing Fe3+/ferricyanide complex, inhibiting nitric oxide. maintained erythrocyte membrane integrity prevented hemolysis. Our results also showed that exerted a significant anti-edematic effect vivo by...
Resistance to 5-Fluorouracil chemotherapy is a major cause of therapeutic failure in colon cancer cure. Development combined therapies constitutes an effective strategy inhibit cells and prevent the emergence drug resistance. For this purpose, we investigated anti-tumoral effect thirteen phenolic compounds, from Tunisian quince Cydonia oblonga Miller, alone or 5-FU, on human 5-FU-resistant LS174-R comparison parental cells. Our results showed that only Kaempferol was able chemo-sensitize...
The emergence of antibiotics-resistant bacteria has been a serious concern for medical professionals over the last decade. Therefore, developing new and effective antimicrobials with modified or different modes action is continuing imperative. In this context, our study focuses on evaluating antimicrobial activity chemically synthesized flavonoids (FLAV) to guide chemical synthesis molecules. A set 12 molecules (4 chalcones, 4 flavones flavanones), bearing substitutions chlorine bromine...
Vascular endothelial growth factor (VEGF) is one of the most important regulators physiological and pathological angiogenesis. Constitutive activation extracellular signal-regulated kinase (ERK) pathway overexpression VEGF are common denominators tumors from different origins. We have established a new link between these two fundamental observations converging on mRNA stability. In this complex phenomenon, tristetraprolin (TTP), an adenylate uridylate-rich element-associated protein that...
Post-transcriptional regulation plays a central role in cell differentiation and proliferation. Among the regulatory factors involved this mechanism, Tristetraprolin (ZFP36 or TTP) is prototype of family RNA-binding proteins that bind to adenylate uridylate (AU)-rich sequences 3'UTR mRNAs, which promotes their physiological decay. Here, we investigated whether TTP correlates with tumor aggressiveness breast cancer novel prognostic factor for neoplasia. By immunoblot analysis, determined...
Lebein, is an heterodimeric disintegrin isolated from Macrovipera lebetina snake venom that was previously characterized as inhibitor of ADP-induced platelet aggregation. In this study, we investigated the effect Lebein on p53-dependent growth human colon adenocarcinoma cell lines. We found significantly inhibited LS174 (p53wt), HCT116 and HT29 (p53mut) cancer viability by inducing cycle arrest through modulation expression levels tumor suppression factor p53, regulating proteins cyclin D1,...
Development of alternative cancer-specific drugs would be paramount importance to overcome toxicity toward normal tissues and tumor resistance. Here, we investigated the potential anti-tumoral effect peel (Peph) pulp polyphenolic extracts from Tunisian quince Cydonia oblonga Miller on both no-tumorigenic cells NIH 3T3 Fibroblasts HEK 293 human colon adenocarcinoma LS174 cells.Cell proliferation cytotoxicity were measured with MTT LDH assays respectively. Cell cycle distribution apoptosis...
Conventional treatment of advanced colorectal cancer is associated with tumor resistance and toxicity towards normal tissues. Therefore, development effective anticancer therapeutic alternatives still urgently required. Nowadays, marine secondary metabolites have been extensively investigated due to the fact that they frequently exhibit anti-tumor properties. However, little attention has given terpenoids isolated from seaweeds. In this study, we halogenated monoterpene mertensene red alga...
We report the chemical composition and anti- Leishmania antioxidant activity of Artemisia campestris L. herba - alba Asso. essential oils (EOs). Our results showed that these extracts exhibit different activities according to used assay. The radical scavenging effects determined by DPPH assay were IC 50 = 3.3 mg/mL 9.1 for oils, respectively. However, both ferric-reducing power (FRAP) assay, in same range (2.3 2.97 mg eq EDTA/g EO, resp.), while oil highest chelating Fe 2+ ions (27.48 mM )....
Background The ambiguous role of transcription factor Sp3 for tumour progression is still debated since it was described as a transcriptional repressor or activator. Here we tried to decipher the molecular mechanisms implicated in accumulation observed aggressive tumours. Methodology We generated normal and cell lines conditionally expressing Sp3. Cell growth analyzed vitro after inoculation nude mice. Apoptosis assessed by pan- caspase activity assays, counting fragmented nuclei...
Melanoma, the most threatening form of skin cancer, has a very poor prognosis and is characterized by its invasive chemoresistant properties. Despite recent promising news from field immunotherapy, there an urgent need for new therapeutic approaches that are free resistance mechanisms side effects. Anti-neoplasic properties have been highlighted different disintegrins snake venom including Lebein; however, exact effect Lebein on melanoma not yet defined. In this study, we showed blocks cell...
Leishmaniases are neglected parasitic diseases in spite of the major burden they inflict on public health. The identification novel drugs and targets constitutes a research priority. For that purpose we used Leishmania infantum initiation factor 4A (LieIF), an essential translation belongs to DEAD-box proteins family, as potential drug target. We modeled its structure identified two binding sites. A virtual screening diverse chemical library was performed for both results were analyzed with...
Use of plant extracts, alone or combined to the current chemotherapy as chemosensitizers, has emerged a promising strategy overcome tumor drug resistance. Here, we investigated anticancer activity Allium roseum L. wild edible species in North Africa, on human Chronic Myeloid Leukemia (CML) K562 cells. The dehydrated aqueous extract (DAE) disturbed cell cycle progression and induced apoptosis Chemical analysis DAE showed diversity organosulfur compounds S-alk(en)yl-cysteine sulfoxides (RCSO)...
The failure of chemotherapy especially in triple negative breast cancer (TNBC) patients has been correlated with the overexpression mesenchymal-epithelial transition factor (c-Met) receptor. Thus, hepatocyte growth (HGF)/c-Met signaling axis gained considerable attention as a valid molecular target for therapy. This study reports first time discovery 131 -oxophorbines pheophorbide A and protopheophorbide along chlorophyllide from Ziziphus lotus, an edible typical Tunisian plant, potent...
Glioblastoma is an aggressive cancer, against which medical professionals are still quite helpless, due to its resistance current treatments. Scorpion toxins have been proposed as a promising alternative for the development of effective targeted glioblastoma therapy and diagnostic. However, exploitation long peptides could present disadvantages. In this work, we identified synthetized AaTs-1, first tetrapeptide from Androctonus australis scorpion venom (Aa), exhibited antiproliferative...
Inflammation is associated with many pathology disorders and the malignant progression of most cancers. Therefore, targeting inflammatory pathways could provide a promising strategy for disease prevention treatment. In this study, we experimentally investigated anti-inflammatory effect CC5 CC8, two disintegrin isoforms isolated from Cerastes cerastes snake venom, on LPS-stimulated macrophages, both human THP-1 mouse RAW264.7 cell adherence their underlying mechanisms by measuring cytokine...