A5054600914- Insect Resistance and Genetics
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Phagocytosis and Immune Regulation
- CAR-T cell therapy research
- Insect and Pesticide Research
- Research in Cotton Cultivation
- Entomopathogenic Microorganisms in Pest Control
- Insect-Plant Interactions and Control
- CRISPR and Genetic Engineering
- Immunotherapy and Immune Responses
- Neurobiology and Insect Physiology Research
- Mycorrhizal Fungi and Plant Interactions
- Transgenic Plants and Applications
- Plant biochemistry and biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Fungal Biology and Applications
- Plant tissue culture and regeneration
- Viral Infectious Diseases and Gene Expression in Insects
- Electrohydrodynamics and Fluid Dynamics
- Insect Utilization and Effects
- Plant Molecular Biology Research
- Invertebrate Immune Response Mechanisms
- Toxin Mechanisms and Immunotoxins
Central Institute for Cotton Research
2016-2024
Indian Institute of Technology Roorkee
2021-2024
Public Health Dayton & Montgomery County
2022
University of Agricultural Sciences, Bangalore
2017
National Institute of Immunology
2006-2016
Kettering University
2009-2016
Dr. Reddy's Laboratories (India)
2016
Memorial Sloan Kettering Cancer Center
2009-2016
Rutgers, The State University of New Jersey
2013
Abstract The broad-complex tramtrack and bric a brac-zinc finger transcriptional regulator(BTB-ZF), promyelocytic leukemia zinc (PLZF), was recently shown to control the development of characteristic innate T cell phenotype effector functions NK cells. Interestingly, ectopic expression PLZF push conventional cells into an activated state that seems be proinflammatory. factors normal in lymphocytes are unknown. In this study, we show is not restricted but also expressed by subset γδ cells,...
Invariant natural killer T cells have a distinct developmental pathway from conventional αβ cells. Here we demonstrate that the transcriptional repressor NKAP is required for invariant cell but not development. In CD4-cre conditional knockout mice, development blocked at double-positive stage. This cell-intrinsic block due to decreased survival or failure rearrange Vα14-Jα18 receptor-α chain, rescued by overexpression of rec-Vα14-Jα18 transgene stage, thus defining role in selection into...
Electrospinning stands out as a flexible and viable method, presenting designed nanoscale materials with customized properties. This research demonstrates the immobilization of carboxylesterase protein Ha006a, reported for its adequacy in pesticide bioremediation by utilizing electrospinning strategy. strategy was utilized to create nanofibers incorporating variable mixtures biodegradable cost-effective polyvinyl alcohol (PVA)-chitosan (CS) nanofiber solution (PVA100, PVA96, PVA94, PVA92...
Helicoverpa armigera (Ha), a polyphagous pest, causes significant damage to several crop plants, including cotton. The control of this cosmopolitan pest is largely challenging due the development resistance existing management practices. Juvenile Hormone (JH) plays pivotal role in life cycle insects by regulating their morphogenetic and gonadotropic development. Hence, enzymes involved JH biosynthesis are an attractive target for selective insecticides. Farnesyl diphosphate synthase (FPPS),...
MHC class II-expressing thymocytes can efficiently mediate positive selection of CD4 T cells in mice. Thymocyte-selected (T-CD4) have an innate-like phenotype similar to invariant NKT cells. To investigate the development and function T-CD4 in-depth, we cloned TCR genes from generated transgenic Remarkably, (T3) occurred more when II was expressed by than thymic epithelial Similar polyclonal also cells, T3 cell is controlled signaling lymphocyte activation molecule/signaling...
Abstract Microinjection is considered as an effective method for dsRNA delivery in insects. It also facilitates the of a precise quantity host insect, inducing efficient RNAi response. However, microinjection needs prior optimization several parameters like concentration dsRNA, site injection, developmental stage insect etc. Moreover, sophisticated devices are largely expensive with high maintenance cost. The Old‐World bollworm, Helicoverpa armigera (Hübner) known to be detrimental...
Enhanced cell death and deficient clearance of cellular debris are thought to contribute increased self-antigen exposure in systemic autoimmune disease. To investigate the characteristics early humoral responses, six monoclonal antibodies were generated from two prone strains mice. All specifically bound surface late-stage apoptotic cells. Similar antibody reactivities present sera patients with lupus erythematosus. While IgM significantly reduced phagocytic uptake thymocytes, IgG enhanced...
While apoptotic debris is believed to constitute the original antigenic insult in lupus (which characterized by a time-dependent diversification of autoreactivity), whether such and autoantibodies specifically recognizing its constituents mediate differential effects on innate humoral responses lupus-prone mice currently unknown. Apoptotic blebs (as opposed cellular lysate) enhanced preferentially maturation dendritic cells (DCs) from bone marrow precursors drawn mice. Murine, somatically...
Asiatic cotton (Gossypium arboreum) cultivated as 'desi cotton' in India, is renowned for its climate resilience and robustness against biotic abiotic stresses. The genome of G. arboreum therefore, considered a valued reserve information discovering novel genes or gene functions trait improvements the present context cultivation world-wide. In study, we carried out genome-wide analysis LIM family desi identified twenty domain proteins (GaLIMs) which include sixteen animals CRP-like GaLIMs...
In this study, the tcdA coding gene of Photorhabdus luminescens was inserted in pET32a (+) plasmid with extra His-tag sequence. strain isolated from Western Ghats Karnataka, which is symbiotically associated entomopathogenic nematode Heterorhabditis spp. The DNA sequence cloned toxin (7551 bp) has an open reading frame encoding 2506 amino acids a predicted molecular mass 283kDa. integrity constructed confirmed using restriction digestion and PCR. expressed after induction IPTG Escherichia...
Estimation of gene expression levels plays a crucial role in understanding the function target gene(s). Intersample variance can be more precisely measured if transcripts are accurately normalized. Normalization is pre-requisite step prior to determination candidate by qPCR. In this study conducted at ICAR-Central Institute for Cotton Research, Nagpur during 2015–16, six reference genes, viz. actin4 (ACT4), actin7(ACT7), RNA Helicase (RNAH), Serine/threonine-protein phosphatase...
Juvenile hormone compounds belong to a family of acyclic sesquiterpenoids, biosynthesized within small paired endocrine glands corpora allata-corpora cardiaca having neural connection with the brain. Stringent regulation JH-levels in insect haemolymph is one most critical factors ensuring normal growth and development insects. JHAMT characterized as key regulatory enzyme playing pivotal role during metamorphosis reproduction physiology by catalyzing final step SAM-dependent methylation...
Abstract Invariant Natural Killer T (NKT) cells, a CD1d-restricted subset of αβ have been increasingly recognized for their role in wide variety functions associated with autoimmunity, cancer, infection, tolerance, and obesity. The semi-invariant TCR expressed by NKT cells is unique its recognition glycolipids presented CD1d, heterodimer rigidity, limited binding footprint compared to conventional TCR:MHC interactions. This distinctive interaction has studied extensively vitro, though the...