A5019379095- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Phagocytosis and Immune Regulation
- Microbial Natural Products and Biosynthesis
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Biochemical and Molecular Research
- Dental materials and restorations
- RNA and protein synthesis mechanisms
- Immune Response and Inflammation
- Nitric Oxide and Endothelin Effects
- Peptidase Inhibition and Analysis
- Systemic Lupus Erythematosus Research
- Genomics and Phylogenetic Studies
- Cardiac Ischemia and Reperfusion
- Immune cells in cancer
- Monoclonal and Polyclonal Antibodies Research
- Meta-analysis and systematic reviews
- Dental Research and COVID-19
- Stroke Rehabilitation and Recovery
- Biomedical and Engineering Education
- Carbohydrate Chemistry and Synthesis
- Epoxy Resin Curing Processes
- Inorganic and Organometallic Chemistry
National Institute of Pharmaceutical Education and Research
2023
Albert Einstein College of Medicine
2009-2021
Taif University
2019
Ubiquitous Energy (United States)
2018
National Institute of Immunology
2004-2016
University of Toronto
1975-2011
Public Health Agency of Canada
2011
Public Health Ontario
2011
Western University
1992
Aurora College
1975-1976
CD1d-restricted natural killer T cells (NKT cells) possess a wide range of effector and regulatory activities that are related to their ability secrete both helper 1 (Th1) cell- Th2 cell-type cytokines. We analyzed presentation NKT cell activating alpha galactosylceramide (alphaGalCer) analogs give predominantly cytokine responses determine how ligand structure controls the outcome activation. Using monoclonal antibody specific for alphaGalCer-CD1d complexes visualize quantitate glycolipid...
Many hematopoietic cell types express CD1d and are capable of presenting glycolipid antigens to invariant natural killer T cells (iNKT cells). However, the question which principal presenters in vivo remains controversial, it has been suggested that this might vary depending on structure a particular antigen. Here we have shown single type cell, CD8α+ DEC-205+ dendritic was mainly responsible for capturing variety different antigens, including multiple forms α-galactosylceramide stimulate...
Structural variants of α-galactosylceramide (αGC) that activate invariant natural killer T cells (iNKT cells) are being developed as potential immunomodulatory agents for a variety applications. Identification specific forms these glycolipids bias responses to favor production proinflammatory vs anti-inflammatory cytokines is central current efforts, but this goal has been hampered by the lack in vitro screening assays reliably predict vivo biological activity compounds. Here we describe...
The study of bioactive natural products has undergone rapid advancement with the cloning and sequencing large number gene clusters concurrent progress to manipulate complex biosynthetic systems in heterologous hosts. genetic reconstitution necessitates that hosts possess substrate pools could be coordinately supplied for biosynthesis. Polyketide synthases (PKS) utilize acyl-coenzyme A (CoA) precursors synthesize polyketides by repetitive decarboxylative condensations. Here we show acyl-CoA...
FadD28 from Mycobacterium tuberculosis belongs to the fatty-acyl AMP ligase (FAAL) family of proteins. It is essential for biosynthesis a virulent phthiocerol dimycocerosate (PDIM) lipid that only found in cell wall pathogenic mycobacteria. The N-terminal domain, comprising first 460 residues, was crystallized by hanging-drop vapour-diffusion method at 295 K. crystals belong space group P212121, with unit-cell parameters = 50.97, b 60.74, c 136.54 Å. crystal structure domain 2.35 Å...
Dendritic cells (DCs) are professional antigen-presenting primarily responsible for acquiring, processing and presenting antigens on antigen molecules to initiate T-cell-mediated immunity. can be separated into several phenotypically functionally heterogeneous subsets. Three important subsets of splenic dendritic plasmacytoid, CD8αPos CD8αNeg cells. The plasmacytoid DCs natural producers type I interferon anti-viral T cell DC subset is specialized MHC class II presentation centrally involved...
Enhanced cell death and deficient clearance of cellular debris are thought to contribute increased self-antigen exposure in systemic autoimmune disease. To investigate the characteristics early humoral responses, six monoclonal antibodies were generated from two prone strains mice. All specifically bound surface late-stage apoptotic cells. Similar antibody reactivities present sera patients with lupus erythematosus. While IgM significantly reduced phagocytic uptake thymocytes, IgG enhanced...
Aim:The aim of this work was to study the effect adding zirconium oxide (ZrO 2 ) nanofiller at different concentrations (0.5 wt%, 1 and 3 wt%) on impact strength (IS) fracture toughness (FT) heat-polymerized polymethylmethacrylate (PMMA).Materials methods: Zirconium added PMMA.Total eighty specimens prepared for both tests, 40 an individual test.For test, four groups were formed (10 each).There three experimental with ZrO powders (0.5%, 1%, 3%) by weight one control group without any...
The undigested remnants of apoptosis are believed to stimulate the generation autoantibodies in lupus. biological properties initiator, disease-specific IgM antibodies that specifically recognize apoptotic cells, readily detected sera lupus patients, remain unclear. Apoptotic cell-reactive monoclonal (generated from lupus-prone mice), as opposed control IgM, preferentially stimulated maturation bone marrow-derived dendritic cells (BMDCs) derived such mice, relative BMDCs healthy mice. An...
The effects of elevated pressures (to 6 atmospheres absolute (ATA)) nitrous oxide (N 2 O) and xenon (Xe), barbiturates on platelet free cytosolic calcium ([Ca 2+ ] i ) aggregation were studied. N O inhibited the ADP-induced rise in [Ca whereas Xe had no effect. Neither affected basal levels. Pentobarbital methohexital little effect or stimulated levels presence "absence" extracellular Ca ; but both, at concentrations > 10 −4 M, induced by adenosine diphosphate. Thiopental increased when...
While apoptotic debris is believed to constitute the original antigenic insult in lupus (which characterized by a time-dependent diversification of autoreactivity), whether such and autoantibodies specifically recognizing its constituents mediate differential effects on innate humoral responses lupus-prone mice currently unknown. Apoptotic blebs (as opposed cellular lysate) enhanced preferentially maturation dendritic cells (DCs) from bone marrow precursors drawn mice. Murine, somatically...