A5091541309- Single-cell and spatial transcriptomics
- Zebrafish Biomedical Research Applications
- Immune cells in cancer
- Pregnancy and preeclampsia studies
- Hematopoietic Stem Cell Transplantation
- Systemic Sclerosis and Related Diseases
- Neonatal Respiratory Health Research
- Blood donation and transfusion practices
- Viral-associated cancers and disorders
- Renal and related cancers
- Pluripotent Stem Cells Research
- Dupuytren's Contracture and Treatments
- Blood groups and transfusion
- Lipid metabolism and disorders
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Parvovirus B19 Infection Studies
- Phagocytosis and Immune Regulation
- Acute Myeloid Leukemia Research
- Herpesvirus Infections and Treatments
University of Washington
2023-2024
Seattle Children's Hospital
2024
Fred Hutch Cancer Center
2019-2022
Abstract Hematopoietic stem cells (HSCs) develop from hemogenic endothelium within embryonic arterial vessels such as the aorta of aorta-gonad-mesonephros region (AGM). To identify signals responsible for HSC formation, here we use single cell RNA-sequencing to simultaneously analyze transcriptional profiles AGM-derived transitioning HSCs, and endothelial which provide sufficient support maturation self-renewal. Pseudotemporal ordering reveals dynamics gene expression during transition,...
Abstract Morphea, also known as localized scleroderma, is an inflammatory sclerosing disorder of uncertain pathogenesis that affects the skin and underlying tissues. In pediatric population, disease often runs a chronic course with high risk for irreversible sequelae; such, patients require long‐term monitoring. The objective this study to develop multi‐center, consensus‐based electronic medical record template morphea patient visits using modified Delphi method iterative surveys. By...
ABSTRACT Haematopoietic stem cells (HSCs) develop from haemogenic endothelium (HE) within embryonic arterial vessels such as the aorta of aorta-gonad-mesonephros region (AGM). To identify signals responsible for HSC formation, we used single cell RNA-sequencing to simultaneously analyze transcriptional profiles AGM-derived transitioning HE HSC, and endothelial which provide sufficient support maturation self-renewal. Pseudotemporal ordering revealed dynamics gene expression during...
Summary Early patterning of mesodermal precursor populations is a key step hematopoietic development in the embryo. To better understand this process, we employed sci-Plex, high-throughput method measuring multiplexed perturbations at single-cell level, to evaluate transcriptional response mouse embryonic stem cells subjected gradient two morphogens early mesoderm/hematopoietic development, Activin and BMP4. sci-Plex revealed varying combinations BMP4 temporally influenced mesoderm vitro...
SUMMARY During embryogenesis, waves of hematopoietic progenitors develop from hemogenic endothelium (HE) prior to the emergence self-renewing stem cells (HSC). Although previous studies have shown that yolk sac-derived erythromyeloid and HSC emerge distinct populations HE, it remains unknown whether earliest lymphoid-competent progenitors, multipotent originate common HE. Here we demonstrate by clonal assays single cell transcriptomics rare HE with functional potential in early murine embryo...
Abstract Dermatologic complications are common following allogeneic hematopoietic stem cell transplantation, but dermatologic among pediatric patients undergoing transplantation for the treatment of sickle disease have been poorly characterized. In this case series 17 (<21 years old) with who underwent 16 (94.1%) experienced one or more after transplant, most including acute chronic mucocutaneous graft‐versus‐host (GVHD) (34.1% complications), skin eruptions unknown origin (15.9%...
Abstract During murine embryonic development, the first hematopoietic stem cells (HSCs) emerge within major arterial vasculature, including aorta-gonad-mesonephros (AGM) region. Throughout their emergence and subsequent maturation, HSCs retain a close physical association with surrounding endothelial cell layer, suggesting that signaling interactions between HSC vascular niche may play an integral role in development. Indeed, we have previously shown co-culture AGM-derived (AGM EC)...
Abstract Our previous studies demonstrated the capacity of a stroma layer consisting AGM-derived myrAKT-transduced endothelial cells (AGM-EC) to support in vitro conversion hemogenic endothelium (HE) hematopoietic stem (HSCs) that functionally reconstitute long-term multilineage hematopoiesis adult recipients following transplantation. Here we present our protocol for an engineered stromal-free niche developed using complementary single cell transcriptomic analyses HE/HSC and AGM-EC define...
During embryogenesis, waves of hematopoietic progenitors develop from hemogenic endothelium (HE) prior to the emergence self-renewing stem cells (HSC). Although previous studies have shown that yolk sac-derived erythromyeloid and HSC emerge distinct populations HE, it remains unknown whether earliest lymphoid-competent progenitors, multipotent originate common HE. Here we demonstrate by clonal assays single cell transcriptomics rare HE with functional potential in early murine embryo are...