A5087071162- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Carcinogens and Genotoxicity Assessment
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Nanoparticles: synthesis and applications
- Ferroptosis and cancer prognosis
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- Acute Myeloid Leukemia Research
- RNA modifications and cancer
- Medicinal Plants and Neuroprotection
- Cellular Mechanics and Interactions
- Hemoglobinopathies and Related Disorders
- Mycobacterium research and diagnosis
- Virus-based gene therapy research
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- CAR-T cell therapy research
- Machine Learning in Bioinformatics
- Protein Tyrosine Phosphatases
- Cancer-related Molecular Pathways
- Cancer, Lipids, and Metabolism
Universiti Sains Malaysia
2016-2025
University Kebangsaan Malaysia Medical Centre
2021
The University of Tokyo
2013-2014
Using quantitative PCR-based miRNA arrays, we comprehensively analyzed the expression profiles of miRNAs in human and mouse embryonic stem (ES), induced pluripotent (iPS), somatic cells. Immature cells were purified using SSEA-1 or SSEA-4 used for profiling. Hierarchical clustering consensus by nonnegative matrix factorization showed two major clusters, ES/iPS other cell groups, as previously reported. Principal components analysis (PCA) to identify that segregate these groups identified...
Hematopoietic stem/progenitor cells (HSPCs) are susceptible to benzene-induced genotoxicity. However, little is known about the mechanism of DNA damage response affecting lineage-committed progenitors for myeloid, erythroid, and lymphoid. Here, we investigated genotoxicity a benzene metabolite, 1,4-benzoquinone (1,4-BQ), in HSPCs using oxidative stress lineage-directed approaches. Mouse bone marrow (BMCs) were exposed 1,4-BQ (1.25–12 μM) 24 h, followed by assessments. Then, was evaluated...
Abstract The oncogene xCT plays an indispensable role in tumor growth by protecting cancer cells from oxidative stress and ferroptosis. Emerging evidence indicated function is tightly controlled posttranslational modifications, especially ubiquitination. However, it still remains unclear what specific regulatory mechanism of ubiquitin ligases human cancers. Here, we reported that NEDD4L, E3 ligases, inhibited esophageal squamous cell carcinoma (ESCC) facilitated ferroptosis ubiquitination...
MicroRNAs are differentially expressed in cells and regulate multiple biological processes. We have been analyzing comprehensive expression patterns of microRNA human mouse embryonic stem induced pluripotent cells. determined microRNAs specifically these cells, miR-142-3p is one such microRNAs. at higher levels relative to fibroblasts mice. Level miR142-3p decreased during embryoid body formation from Loss-of-function analyses suggested that plays roles the proliferation differentiation CpG...
MicroRNAs (miRNAs) are short stranded noncoding RNA that play important roles in apoptosis, cell survival, development and proliferation. However, gene expression control via small regulatory RNA, particularly miRNA breast cancer is still less explored. Therefore, this project aims to develop an approach target microRNA-130a using the Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9 system MCF7, line. The 20 bp sequences at stem loop, 3ʹ 5ʹ end of miR130a were cloned...
Background HSPCs are targets for benzene-induced hematotoxicity and leukemogenesis. However, benzene toxicity targeting microRNAs (miRNAs) transcription factors (TF) that involve in regulating self-renewing differentiation of comprising different hematopoietic lineages remains poorly understood. In this study, the effect a metabolite, 1,4-benzoquinone (1,4-BQ) exposure, focusing on (miRNAs: miR-196b miR-29a; TF: HoxB4, Bmi-1) miR-181a, GATA3) pathways were investigated. Methods Freshly...
BACKGROUND: Molecular alterations of microRNA130a (miR130a) are observed in many types cancers, including non-small cell lung cancer (NSCLC). However, the role miR130a NSCLC has been poorly studied.METHODS: In this study, clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 was utilised to knockdown miR130a. The gRNA designed target stem loop, 3’ and 5’ sites stably expressed A549 cells. Post-treatment, mature levels miR130a-3p 5p were quantified, proliferation migration...
Green synthesis is preferable for the of silver nanoparticles (AgNPs) due to its rapid and reliable process. Previous studies have demonstrated that active compounds present in Vernonia cinerea (V. cinerea) are responsible bioreduction during AgNPs. Hence, this study, aqueous extract V. was used synthesise The added AgNO3 solution (25 mL, 50 mL 100 mL) produce mixture with ratio 1:5, 1:10 1:20. formation cinerea-AgNPs monitored colour changes, characterised by UV-Vis spectroscopy,...
Abstract Background and objectives: Haemoglobin E β-thalassaemia has a variable clinical presentation. This study describes the spectrum of these patients in two thalassaemia centers Malaysia addition to determining prevalence selected primary secondary genetic modifiers which may influence its phenotype. Methods: A total 99 were recruited this cross-sectional study. Clinical parameters severity scoring determined. Molecular analysis was performed: Sanger sequencing MLPA for β globin...
Clustered Regularly Interspaced Palindromic Repeats (CRISPR) technology is an innovative gene-editing technique that has emerged as a result of the development genetic engineering.This expanded scope oncology-related medical research and clinical applications.CRISPR used in molecular techniques to decode genes pathways, alter expression specific for therapeutic purposes, comprehend pathophysiology cancer.If pre-clinical with this successful, it could lead trials eventually be therapy.To...
Event Abstract Back to Mechanism of 1,4-benzoquinone-induced genotoxicity in haematopoietic stem/ progenitor cells: linking benzene haematological disorders Zariyantey Abdul Hamid1*, Paik Wah Chow1, Ramya Dewi Mathialagan1, Nor Fadilah Rajab1, Salwati Shuib2 and Siti Razila Razak3 1 Faculty Health Sciences, National University Malaysia, Malaysia 2 Medical Center, 3 Cluster Oncological Radiological Science, Advanced Dental Institute, Background Haematopoietic cells (HSPCs) are sensitive...