A5025616083- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- COVID-19 diagnosis using AI
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Parasites and Host Interactions
- Parasitic infections in humans and animals
- Parasite Biology and Host Interactions
- COVID-19 and healthcare impacts
- Chronic Lymphocytic Leukemia Research
- T-cell and Retrovirus Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- RNA and protein synthesis mechanisms
- Neonatal and Maternal Infections
- Genomics and Chromatin Dynamics
- Streptococcal Infections and Treatments
- Infectious Disease Case Reports and Treatments
- Urologic and reproductive health conditions
- Biosensors and Analytical Detection
- Sarcoidosis and Beryllium Toxicity Research
- RNA Interference and Gene Delivery
University of Cambridge
2004-2023
Cambridge University Hospitals NHS Foundation Trust
2020-2023
Addenbrooke's Hospital
2004-2021
There is an urgent need for rapid SARS-CoV-2 testing in hospitals to limit nosocomial spread. We report evaluation of point care (POC) nucleic acid amplification (NAAT) 149 participants with parallel combined nasal and throat swabbing POC versus standard lab RT-PCR testing. Median time result 2.6 (IQR 2.3–4.8) 26.4 h 21.4–31.4, p < 0.001), 32 (21.5%) positive 117 (78.5%) negative. Cohen's κ correlation between tests 0.96 (95% CI 0.91–1.00). When comparing nearly 1,000 pre-...
The Gag polyprotein is the major structural protein of human immunodeficiency virus‐1 (HIV‐1) constituting viral core. Between translation on cytoplasmic polysomes and assembly into particles at plasma membrane, it specifically captures RNA genome virus through binding motifs (packaging signals –Ψ) in RNA. believed to be a facilitator assembly. Using combined approach immunofluorescence detection situ hybridisation genomic RNA, we demonstrate that colocalises early after expression with Ψ+...
Abstract Background Sparganosis is an infection with a larval Diphyllobothriidea tapeworm. From rare cerebral case presented at clinic in the UK, DNA was recovered from biopsy sample and used to determine causative species as Spirometra erinaceieuropaei through sequencing of cox1 gene. same DNA, we have produced draft genome, first its kind for this species, it perform comparative genomics analysis investigate known potential tapeworm drug targets Results The 1.26 Gb genome S. currently...
Abstract Cure of Human Immunodeficiency Virus (HIV) infection remains elusive due to the persistence HIV in a latent reservoir. Strategies eradicate can only be evaluated with robust, sensitive and specific assays quantitate reactivatable virus. We have taken standard peripheral blood mononuclear cell (PBMC) based viral outgrowth methodology from it created logistically simpler more highly reproducible assay quantify replication-competent resting CD4 + T cells, both increasing accuracy...
Immune reconstitution inflammatory syndrome is a recognized complication after the initiation of combination antiretroviral therapy (cART). We report patient who developed life-threatening pulmonary immune (IRIS) three days cART. reviewed published cases IRIS Pneumocystis pneumonia (PCP), in particular time from cART to event. The median duration onset was 15 33 patients reviewed. This alerts clinicians rapidity development following PCP
Transcriptionally silent HIV proviruses form the major obstacle to eradicating HIV. Many studies of latency have focused on cellular mechanisms that maintain silencing proviral DNA. Here we show viral sequence variation affecting replicative ability leads variable rates and reactivate. We studied naturally occurring engineered polymorphisms in a recently identified exonic splice enhancer (ESEtat) regulates tat mRNA splicing constructed viruses with increased (strain M1), reduced M2), or...
Abstract Background There is urgent need for safe and efficient triage protocols hospitalized COVID-19 suspects to appropriate isolation wards. A major barrier timely discharge of patients from the emergency room hospital turnaround time many SARS-CoV-2 nucleic acid tests. We validated a point care amplification based platform SAMBA II diagnosis performed an implementation study assess its impact on patient disposition at academic hospital. Methods prospectively recruited admitted (...
Abstract The persistence of infected T cells harbouring intact HIV proviruses is the barrier to eradication HIV. This reservoir stable over long periods time despite antiretroviral therapy. There has been controversy on whether low level viral replication occurring at sanctuary sites periodically reseeding into latent account its durability. To study evolution in a physiologically relevant population viruses, we repeatedly performed virus outgrowth assays stably treated positive patient two...
Sparganosis is an infection with a larval Diphyllobothriidea tapeworm. From rare cerebral case presented at clinic in the UK, DNA was recovered from biopsy sample and used to determine causative species as Spirometra erinaceieuropaei through sequencing of cox1 gene. same DNA, we have produced draft genome, first its kind for this species, it perform comparative genomics analysis investigate known potential tapeworm drug targets The 1.26 Gb genome S. currently largest reported any flatworm....
Abstract Understanding the mechanisms involved in HIV infection and latency, development of a cure, rely on availability sensitive research tools such as indicator cells, which allow rigorous quantification viral activity. Here we describe construction validation novel dual-indicator cell line, Sup-GGR, offers two different readouts to quantify replication. A construct expressing both Gaussia luciferase hrGFP Tat- Rev-dependent manner was engineered into SupT1-CCR5 create Sup-GGR cells. This...
Without universal access to point-of-care SARS-CoV-2 testing, many hospitals rely on clinical judgement alone for identifying cases of COVID-19 early.
Proviral gene expression is a critical step in the retroviral life cycle and an important determinant efficiency of retrovirus based therapy vectors. There as yet no method described that can assess proviral while vigorously excluding contribution from unstable species such passively transferred plasmid LTR circles. Here, we present achieve this. was detected by activity puromycin resistance encoded viral vector, quantified comparing growth curve sample under selection to series calibration...
An 80-year-old diabetic female was electively admitted for an anterior resection of adenocarcinoma the rectum. Two weeks after operation she complained lower abdominal pain, malaise, and feeling generally unwell, having made good initial progress. She had a pulse 100 bpm, temperature 38.5 °C, stable blood pressure. Examination revealed very tender abdomen but no evidence peritonitis. looked unwell transferred to intensive care unit closer observation. A pelvic computed tomography (CT) showed...
Abstract Background Poststreptococcal glomerulonephritis (PSGN) is caused by prior infection with specific strains of group A streptococcus (GAS). PSGN commonly characterised significant complement C3 depression and maintained C4 levels, especially in children. The 2022-23 GAS outbreak has been associated childhood morbidity mortality the UK due to invasive infections but no described. Methods We reviewed laboratory requests for measurements received main East England immunology 48 months...