A5017584434- Effects and risks of endocrine disrupting chemicals
- Birth, Development, and Health
- Pancreatic function and diabetes
- Toxic Organic Pollutants Impact
- Endoplasmic Reticulum Stress and Disease
- Thyroid Disorders and Treatments
- Environmental Toxicology and Ecotoxicology
- Carcinogens and Genotoxicity Assessment
- Estrogen and related hormone effects
- HIV Research and Treatment
- Adipose Tissue and Metabolism
- CRISPR and Genetic Engineering
- Developmental Biology and Gene Regulation
- Genomics and Chromatin Dynamics
- CAR-T cell therapy research
- Animal testing and alternatives
- Electrochemical sensors and biosensors
- Pesticide Exposure and Toxicity
- HIV/AIDS Research and Interventions
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Biomarkers in Disease Mechanisms
- Nerve injury and regeneration
- Reproductive biology and impacts on aquatic species
- Wnt/β-catenin signaling in development and cancer
- RNA Interference and Gene Delivery
Biogem
2012-2023
Wellcome Sanger Institute
2018-2023
University of Bari Aldo Moro
2022
Stazione Zoologica Anton Dohrn
2012-2015
University of Naples Federico II
2009-2012
This study explored the role of irisin as a new pancreatic β-cell secretagogue and survival factor its potential in communication between skeletal muscle β-cells under lipotoxic conditions. Recombinant stimulated insulin biosynthesis glucose-stimulated secretion (GSIS) PKA-dependent manner prevented saturated fatty acid-induced apoptosis human rat β-cells, well murine islets, via AKT/BCL2 signaling. Treatment myotubes with 0.5 mmol/L palmitate for 4 h, but not oleate, promoted an increase...
Loss of IL-10 signaling in macrophages (Mφs) leads to inflammatory bowel disease (IBD). Induced pluripotent stem cells (iPSCs) were generated from an infantile-onset IBD patient lacking a functional IL10RB gene. Mφs differentiated IL-10RB−/− iPSCs lacked IL-10RB mRNA expression, unable phosphorylate STAT3, and failed reduce LPS induced cytokines the presence exogenous IL-10. exhibited striking defect their ability kill Salmonella enterica serovar Typhimurium, which was rescuable after...
Abstract Epidemiologic and experimental studies have associated changes of blood glucose homeostasis to Bisphenol A (BPA) exposure. We took a toxicogenomic approach investigate the mechanisms low-dose (1 × 10 −9 M) BPA toxicity in ex vivo cultures primary murine pancreatic islets hepatocytes. Twenty-nine inhibited genes were identified none exposed Although their expression was slightly altered, impaired cellular level, as whole, resulted specific phenotypic changes. Damage mitochondrial...
Abstract The brain‐derived neurotrophic factor ( BDNF ) gene is expressed in differentiating and post‐mitotic neurons of the zebrafish embryo, where it has been implicated Huntington's disease. Little known, however, about full complement neuronal cell types that express this important vertebrate model. Here, we further explored transcriptional profiles during first week development using real‐time quantitative polymerase chain reaction (RT‐ qPCR whole‐mount situ hybridization (WISH). RT‐...
Epidemiological and experimental data highlighted the thyroid-disrupting activity of bisphenol A (BPA). Although pivotal to identify mechanisms toxicity, direct low-dose BPA effects on thyrocytes have not been assessed. Here, we report results microarray experiments revealing that transcriptome reacts dynamically exposure, adapting changes in gene expression exposure duration. The response involves many genes, enriching specific pathways biological functions mainly cell death/proliferation...
The progressive and physiological decline in ovarian function depends on the rate of follicular loss by atresia, contributing to reduction reserve. Genetics environmental factors play important roles senescence onset dysfunctions such as diminished A better understanding mechanisms underlying aging their regulation genetic is needed evaluate reserve predict fertility potential identification more accurate less invasive markers. We report transcriptomic data (i) implicating novel (e.g. EIF2...
Abstract In vitro Omics analysis (i.e. transcriptome) is suggested to predict in vivo toxicity and adverse effects humans, although the causal link between high-throughput data not easily established. Indeed, chemical-organism interaction can involve processes, such as adaptation, established cell cultures. Starting from this consideration we investigate transcriptomic response of immortalized thyrocytes ethylenthiourea chlorpyrifos. revealed specific common genes/mechanisms toxicity,...
chlorpyrifos (CPF) is an organophosphate insecticide used to control pests on a variety of food and feed crops. In mammals, maternal exposure CPF has been reported induce cerebral cortex thinning, alteration long-term brain cognitive function, Parkinson-like symptoms, but the mechanisms these processes are not fully understood. this study, we aimed gain deeper understanding alterations induced in brains mice chronically exposed by dietary intake. For our purpose, analysed F1 offspring...
Gene editing technologies hold promise for enabling the next generation of adoptive cellular therapies. In conventional gene platforms that rely on nuclease activity, such as clustered regularly interspaced short palindromic repeats CRISPR-associated protein 9 (CRISPR-Cas9), allow efficient introduction genetic modifications; however, these modifications occur via DNA double-strand breaks (DSBs) and can lead to unwanted genomic alterations genotoxicity. Here, we apply a novel modular RNA...
Thyroid hormones (THs) regulate many biological processes in vertebrates, including reproduction. Testicular somatic and germ cells are equipped with the arrays of enzymes (deiodinases), transporters, receptors necessary to locally maintain optimal level THs their signalling, needed for functions spermatogenesis. Pesticides, as chlorpyrifos (CPF) ethylene thiourea (ETU), impair function thyroid testis, affecting male fertility. However, ability disarrange testicular T3 (t-T3) metabolism...
A deranged differentiation is often a landmark of transformed cells. We used thyroid cell line expressing an inducible Ras oncoprotein in order to study the hierarchy molecular events leading suppression thyroid-specific gene expression. find that, upon activation, there immediate global down-regulation differentiation, which associated with inhibition cAMP signaling pathway. demonstrate that unusual negative cross talk between oncogene and pathway induces inactivation transcription factor...
The paired-type homeodomain transcription factor Uncx is involved in multiple processes of embryogenesis vertebrates. Reasoning that zebrafish genes uncx4.1 and uncx are orthologs mouse , we studied their genomic environment developmental expression. Evolutionary analyses indicate the as being paralogs deriving from teleost-specific whole-genome duplication. Whole-mount situ mRNA hybridization transcripts embryos reveals novel expression domains, confirms those previously known, suggests...
We evaluated the role of p66Shc redox adaptor protein in pancreatic β-cell insulin resistance that develops under lipotoxic conditions and with excess body fat. Prolonged exposure to palmitate vitro or presence overweight/obesity augmented expression levels caused an impaired ability exogenous increase cellular content secreted C-peptide INS-1E cells human murine islets. In cells, knockdown resulted enhanced insulin-induced augmentation secretion prevented impair these effects insulin....
Abstract Genetic and environmental factors contribute to thyroid diseases. Although still debated, 2,3,7,8-tetrachlorodibenzo- p -dioxin (TCDD) is thought induce dysfunction in humans rodents. The data here reported point out the contribution of exposure window genetic background mediating low-dose TCDD effects on thyroid. Indeed, early (from E0.5 PND30) (0,001 μg/kg/day) reduced circulating fT4 altered expression specific transcripts. role components was estimated monitoring same markers...
Epidemiological and experimental studies emphasize the link between environmental chemicals exposure thyroid cancer. However, this association is strongly debated mechanisms of action carcinogens still need to be identified. The analysis in vitro transcriptomic data developed investigate effects chlorpyrifos on immortalized thyrocytes highlighted impaired expression genes involved endodermal carcinogenesis. This carcinogenic gene-network (ECGN, including Zfp36l2, Dmbt1, Ddit4), was validated...
Comparison of toxicogenomic data facilitates the identification deregulated gene patterns and maximizes health risk prediction in human. Here, we performed phenotypic anchoring on effects acute exposure to low-grade polluted groundwater using mouse zebrafish. Also, evaluated two windows chronic mouse, starting utero at end lactation. Bioinformatic analysis livers microarray showed that number biofunctions pathways is higher after exposure, compared one. It also revealed specific profiles...
Early life exposure to Endocrine Disruptor Chemicals (EDCs), such as the organophosphate pesticide Chlorpyrifos (CPF), affects thyroid activity and dependent process, including glucose metabolism. The damage of hormones (THs) a mechanism action CPF is underestimated because studies rarely consider that TH levels signaling are customized peripherally. Here, we investigated impairment metabolism/signaling THs lipid/glucose metabolism in livers 6-month-old mice, developmentally lifelong exposed...