A5025298255- Genetic Mapping and Diversity in Plants and Animals
- Lung Cancer Treatments and Mutations
- Gut microbiota and health
- RNA modifications and cancer
- Genetic and phenotypic traits in livestock
- Diet and metabolism studies
- HER2/EGFR in Cancer Research
- Colorectal Cancer Treatments and Studies
- Animal Genetics and Reproduction
- Salmonella and Campylobacter epidemiology
- Adipose Tissue and Metabolism
- Genomics and Chromatin Dynamics
- Gene expression and cancer classification
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Probiotics and Fermented Foods
- Bioinformatics and Genomic Networks
- Carcinogens and Genotoxicity Assessment
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Genetic factors in colorectal cancer
- Genetic diversity and population structure
- Pharmacogenetics and Drug Metabolism
- CRISPR and Genetic Engineering
Texas A&M University
2016-2025
Mitchell Institute
2015-2025
College Station Medical Center
2024
North Carolina State University
2009-2023
University of North Carolina at Chapel Hill
2005-2023
UNC Lineberger Comprehensive Cancer Center
2003-2023
Texas A&M Health Science Center
2014-2023
Lawrence Berkeley National Laboratory
2023
Texas A&M University System
2023
Pediatrics and Genetics
2003-2023
Gene targeting was used to create a null allele at the epidermal growth factor receptor locus ( Egfr ). The phenotype dependent on genetic background. EGFR deficiency CF-1 background resulted in peri-implantation death due degeneration of inner cell mass. On 129/Sv background, homozygous mutants died mid-gestation placental defects; CD-1 lived for up 3 weeks and showed abnormalities skin, kidney, brain, liver, gastrointestinal tract. multiple associated with indicate that is involved wide...
Whether dietary fiber protects against colorectal cancer is controversial because of conflicting results from human epidemiologic studies. However, these studies and mouse models have not controlled the composition gut microbiota, which ferment into short-chain fatty acids such as butyrate. Butyrate noteworthy it has energetic epigenetic functions in colonocytes tumor-suppressive properties cell lines. We used gnotobiotic colonized with wild-type or mutant strains a butyrate-producing...
The Collaborative Cross (CC) is a mouse recombinant inbred strain panel that being developed as resource for mammalian systems genetics. Here we describe an experiment uses partially CC lines to evaluate the genetic properties and utility of this emerging resource. Genome-wide analysis incipient strains reveals high diversity, balanced allele frequencies, dense, evenly distributed recombination sites—all ideal qualities genetics We map discrete, complex, biomolecular traits contrast two...
Abstract Background The expression of carcino-embryonic antigen by colorectal cancer is an example oncogenic activation embryonic gene expression. Hypothesizing that oncogenesis-recapitulating-ontogenesis may represent a broad programmatic commitment, we compared patterns human cancers (CRCs) and mouse colon tumor models to those development days 13.5-18.5. Results We report here 39 tumors from four independent 100 CRCs encompassing all clinical stages shared striking recapitulation Compared...
In the preovulatory ovarian follicle, mammalian oocytes are maintained in prophase meiotic arrest until luteinizing hormone (LH) surge induces reentry into first division.Dramatic changes somatic cells surrounding and follicular wall also induced by LH necessary for ovulation.Here, we provide genetic evidence that LH-dependent transactivation of epidermal growth factor receptor (EGFR) is indispensable oocyte cell cycle, synthesis extracellular matrix causes cumulus expansion, follicle...
The mechanisms by which angiotensin II (Ang II) promotes renal fibrosis remain incompletely understood. Ang both stimulates TGFβ signaling and activates the EGF receptor (EGFR), but relative contribution of these pathways to fibrogenesis is unknown. Using a murine model with EGFR-deficient proximal tubules, we demonstrate that upstream activation EGFR-dependent ERK critical for mediating sustained expression in fibrosis. Persistent stimulated ROS-dependent phosphorylation Src, leading...
The genomic arrangement of the major bovine milk protein genes has been determined using a combination physical mapping techniques. proteins consist four caseins, α s1 (CASAS1), s2 (CASAS2), β (CASB), and ϰ (CASK), as well two whey proteins, α-lactalbumin (LALBA) β-lactoglobulin (LGB). A panel X hamster hybrid somatic cells analyzed for presence or absence specific restriction fragments revealed coding to reside on three chromosomes. caseins were assigned syntenic group U15 localized...
Complex traits and disease comorbidity in humans model organisms are the result of naturally occurring polymorphisms that interact with each other environment. To ensure availability resources needed to investigate biomolecular networks systems-level phenotypes underlying complex traits, we have initiated breeding a new genetic reference population mice, Collaborative Cross. This has been designed optimally support systems genetics analysis. Its novel important features include high level...
We used the hypomorphic Egfr wa2 allele to genetically examine impact of impaired epidermal growth factor receptor (Egfr) signaling on Apc Min mouse model familial adenomatous polyposis. Transfer onto a homozygous background results in 90% reduction intestinal polyp number relative mice carrying wild-type allele. This effect is potentially synergistic with actions modifier-of-min ( Mom1 ) locus. Surprisingly, size, expansion, and pathological progression polyps appear Egfr-independent....
Mouse genetic resources include inbred strains, recombinant lines, chromosome substitution heterogeneous stocks, and the Collaborative Cross (CC). These were generated through various breeding designs that potentially produce different architectures, including level of diversity represented, spatial distribution variation, allele frequencies within resource. By combining sequencing data for 16 strains recorded history related architecture variation in mouse was determined. The most commonly...
ABSTRACT Iron affects the physiology of bacteria in two different ways: as a micronutrient for bacterial growth and catalyst formation hydroxyl radicals. In this study, we used DNA microarrays to identify C. jejuni genes that have their transcript abundance affected by iron availability. The levels 647 were after addition iron-limited cells. Several classes revealed within 15 min, including immediate-early response well those specific acquisition metabolism. contrast, only 208 differentially...
Genetic variation contributes to host responses and outcomes following infection by influenza A virus or other viral infections. Yet narrow windows of disease symptoms confounding environmental factors have made it difficult identify polymorphic genes that contribute differential in human populations. Therefore, control for these variables a system models the levels genetic diversity found outbred populations such as humans, we used incipient lines highly genetically diverse Collaborative...
Emerging evidence has implicated reactive oxygen species (ROS) in the pathogenesis of inflammatory bowel disease (IBD). Although intestinal epithelial cells produce ROS-neutralizing enzyme superoxide dismutase (SOD), protein and activity levels copper/zinc (Cu/Zn) manganese (Mn) SOD are perturbed inflamed tissues IBD patients. Thus we investigated ability MnSOD from Streptococcus thermophilus to reduce colitis symptoms interleukin (IL) 10-deficient mice using Lactobacillus gasseri as a...