A5017534042- Cell Adhesion Molecules Research
- Platelet Disorders and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Acute Lymphoblastic Leukemia research
- Wnt/β-catenin signaling in development and cancer
- Blood properties and coagulation
- Immunotherapy and Immune Responses
- Cancer-related gene regulation
- Chronic Lymphocytic Leukemia Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Acute Myeloid Leukemia Research
- Extracellular vesicles in disease
- Caveolin-1 and cellular processes
- Glycosylation and Glycoproteins Research
- Chronic Myeloid Leukemia Treatments
- RNA Interference and Gene Delivery
- Microbial Community Ecology and Physiology
- Cellular Mechanics and Interactions
- Malaria Research and Control
- Hepatitis B Virus Studies
- Protist diversity and phylogeny
- Lipid Membrane Structure and Behavior
- Kruppel-like factors research
- Protein purification and stability
Inserm
2014-2023
Université Paris-Saclay
2008-2023
Hôpital Paul-Brousse
2005-2022
Université Paris-Sud
2012-2021
Hôpital Necker-Enfants Malades
2019
Université Paris Cité
2003-2018
Centre National de la Recherche Scientifique
1987-2018
Assistance Publique – Hôpitaux de Paris
2006-2018
Délégation Paris 5
2018
Weatherford College
2012
CD9 is a widely expressed cell surface molecule that belongs to the tetraspanin superfamily of proteins. The tetraspanins CD9, KAI-1/CD82, and CD63 are involved in metastasis suppression, an effect may be related their association with β1 integrins. Knockout mice lacking were created evaluate physiological importance CD9. −/− females displayed severe reduction fertility. Oocytes ovulated but not successfully fertilized because sperm did fuse oocytes from females. Thus, appears essential for...
We analyzed the benefits of a risk-adapted postremission strategy in adult lymphoblastic leukemia (ALL), and re-evaluated stem-cell transplantation (SCT) for high-risk ALL.A total 922 patients entered onto trial according to risk groups: standard-risk ALL (group 1), 2), Philadelphia chromosome-positive 3), CNS-positive 4). All received standard four-drug/4-week induction course. Patients from group 1 who achieved complete remission (CR) after one course therapy were randomly assigned between...
Abstract CD9, CD63, CD81, and CD82 are glycoproteins of unknown function which belong to the tetraspan superfamily. These molecules have short cytoplasmic sequences, four transmembrane domains two unequal extracellular regions. Here, we show that these associated with each other on cell surface such as very late antigen (VLA) integrins HLA‐DR antigens. Moreover, VLA antigens were also found be associated. The interactions analyzed by transfection experiments. It is demonstrated...
The monoclonal antibody ALB 6 directed against the leukocyte differentiation antigen CD9 (p24) increases calcium incorporation into isolated platelet membrane vesicles enriched in internal membranes. similarities of effects both and catalytic subunit cAMP‐dependent protein kinase (C. subunit), which phosphorylates a an apparent molecular mass 23 kDa, led us to investigate relationship between 23‐kDa phosphoprotein (p23). IgG does not inhibit C.subunit‐induced phosphorylation p23...
By associating with specific partner molecules and each other, the tetraspanins are thought to assemble multimolecular complexes that may be especially relevant respect metastasis. We have previously identified a 135-kDa molecule (CD9P-1) as major molecular of CD9 in cancer cell lines. This was identified, after immunoaffinity purification mass spectrometry analysis, protein encoded by the<i>KIAA1436</i> gene human ortholog rat known FPRP. Cross-linking experiments detected complex size plus...
The tetraspanins associate with various surface molecules and each other to build a network of molecular interactions, the tetraspanin web. interaction seems be central for assembly All studied, CD9, CD37, CD53, CD63, CD81, CD82 CD151, were found incorporate [ 3 H]palmitate. By site‐directed mutagenesis, CD9 was palmitoylated at any four internal juxtamembrane regions. palmitoylation did not influence partition in detergent‐resistant membranes but contributed CD81 CD53. In particular,...
The tetraspans are molecules with four transmembrane domains which engaged in multimolecular complexes (the tetraspan web) containing a subset of beta1 integrins (in particular alpha3beta1, alpha4beta1 and alpha6beta1), MHC antigens several unidentified molecules. associated readily detected after immunoprecipitation performed mild detergents such as Brij 97 or CHAPS. In this study we show that another classical detergent, digitonin, dissociated most these molecules, including integrins,...
Abstract By interacting with each others, the tetraspanins are thought to assemble a network of molecular interactions, tetraspanin web. These tetraspanin/tetraspanin interactions involve in part palmitoylation proteins. We show that interact cholesterol as indicated by precipitation complexes digitonin, cholesterol‐precipitating reagent, and labeling CD9, CD81 CD82 photoactivatable vivo . Cholesterol may participate interaction other since digitonin‐precipitation was correlated their mutual...
The CD9 antigen was described originally as a 24-kDa surface protein of non-T acute lymphoblastic leukemia cells and developing B-lymphocytes. It is also strongly expressed on platelets, among other cells, where it shows the property mediating platelet activation aggregation upon binding with mAbs. primary structure has been elucidated by cloning cDNA from lambda gt11 expression vector library constructed megakaryocytic mRNA. Monoclonal antibodies were used probes an APAAP amplification...
In a prospective multicenter study, we analyzed the benefits of allogeneic bone marrow transplantation (BMT) in nonselected group adult patients with acute lymphoblastic leukemia (ALL) and, by randomized trial, evaluated effectiveness autologous BMT over chemotherapy as postremission therapy younger than 50 years who were not candidates for BMT.After induction that to receive one two anthracycline-containing regimens, either daunorubicin (DNR) or zorubicin (ZRB), assigned treatment according...
The metalloprotease ADAM10/Kuzbanian catalyzes the ligand-dependent ectodomain shedding of Notch receptors and activates Notch. Here, we show that human tetraspanins evolutionary conserved TspanC8 subfamily (Tspan5, Tspan10, Tspan14, Tspan15, Tspan17, Tspan33) directly interact with ADAM10, regulate its exit from endoplasmic reticulum, four them ADAM10 surface expression levels. In an independent RNAi screen in Drosophila, two genes were identified as regulators. Functional analysis three...
Abstract Several cytokines and growth factors are released by proteolytic cleavage of a membrane-anchored precursor, through the action ADAM (a disintegrin metalloprotease) metalloproteases. The activity these proteases is regulated largely unknown mechanisms. In this study we show that Ab engagement several tetraspanins (CD9, CD81, CD82) increases epidermal factor and/or TNF-α secretion mechanism dependent on ADAM10. effect anti-tetraspanin mAb release rapid, not relayed intercellular...
Tetraspanins regulate cell migration, sperm–egg fusion, and viral infection. Through interactions with one another other surface proteins, tetraspanins form a network of molecular called the tetraspanin web. In this study, we use single-molecule fluorescence microscopy to dissect dynamics partitioning CD9. We show that lateral mobility CD9 in plasma membrane is regulated by at least two modes interaction each exhibit specific dynamics. The majority molecules display Brownian behavior but can...
The metalloprotease ADAM10 mediates the shedding of ectodomain various cell membrane proteins, including APP, precursor amyloid peptide Aβ, and Notch receptors following ligand binding. associates with members an evolutionary conserved subgroup tetraspanins, referred to as TspanC8, which regulate its exit from endoplasmic reticulum. Here we show that 4 these TspanC8 (Tspan5, Tspan14, Tspan15 Tspan33) positively surface expression levels differentially impact ADAM10-dependent activation...
The tetraspans are molecules with four transmembrane domains which engaged in multimolecular complexes (the tetraspan web) containing a subset of β1 integrins (in particular α3β1, α4β1 and α6β1), MHC antigens several unidentified molecules. associated readily detected after immunoprecipitation performed mild detergents such as Brij 97 or CHAPS. In this study we show that another classical detergent, digitonin, dissociated most these molecules, including integrins, from the CD9, CD37, CD53,...