A5010692870- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Pesticide and Herbicide Environmental Studies
- Hemoglobin structure and function
- Weed Control and Herbicide Applications
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Peroxisome Proliferator-Activated Receptors
- Forensic Toxicology and Drug Analysis
- RNA Interference and Gene Delivery
- Alcohol Consumption and Health Effects
- Lipid Membrane Structure and Behavior
- Virus-based gene therapy research
- Ion-surface interactions and analysis
- Protein Interaction Studies and Fluorescence Analysis
- Synthetic Organic Chemistry Methods
- Stellar, planetary, and galactic studies
- Chemical Synthesis and Analysis
- thermodynamics and calorimetric analyses
- Invertebrate Immune Response Mechanisms
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Cellular transport and secretion
Carleton University
2014-2025
Carleton College
2025
University of Ottawa
2008
York University
2004
Trent University
2001
Parkinson’s disease (PD) is a progressive neurodegenerative affecting the nigrostriatal pathway, where patients do not manifest motor symptoms until >50% of neurons are lost. Thus, it great importance to determine early neuronal changes that may contribute progression. Recent attention has focused on lipids and their role in pro- anti-apoptotic processes. However, information regarding lipid alterations animal models PD lacking. In this study, we utilized high performance liquid...
A novel mass spectrometry (MS)-based lipidomics strategy that exposes glycerophospholipids to an ethereal solution of diazomethane and acid, derivatizing them contain a net fixed, permanent positive charge, is described. The sensitivity modified lipids MS detection enhanced via improved ionization characteristics as well consolidation ion dissociation form one or two strong, characteristic polar headgroup fragments. Our has been optimized enable priori prediction fragmentation patterns for...
Salt cluster ions of alkali metal chlorides ACl (A = Li(+), Na(+), K(+), Rb(+) and Cs(+)) sodium salts NaB (B I(-), HCOO(-), CH(3)COO(-), NO(2)(-), NO(3)(-)), formed by electrospray ionization, were studied systematically mass spectrometry. The influences on the total positive ion negative currents variation solvent, solution concentration, desolvation temperature, flow-rate, capillary voltage cone investigated. Only was found to influence dramatically distribution salt in spectra observed....
Glyphosate (GLY), a synthetic, nonselective systemic herbicide that is particularly effective against perennial weeds, the most used weedkiller in world. There are growing concerns over GLY accumulation environment and attendant human health-associated risks, despite increased attention media, its breakdown product aminomethylphosphonic acid (AMPA) remain elusive to many analytical strategies. Chemical derivatization coupled with high-performance liquid chromatography-mass spectrometry...
Significant sensitivity enhancements in the tandem mass spectrometry-based analysis of complex mixtures several phospholipid classes has been achieved via (13)C-TrEnDi. (13)C-TrEnDi-modified phosphatidylethanolamine (PE), phosphatidylserine (PS), and phosphatidylcholine (PC) lipids extracted from HeLa cells demonstrated greater precursor ion scans (PISs) than their unmodified counterparts. Sphingomyelin (SM) species exhibited neither an increased nor decreased following modification. The use...
Abstract The use of engineered viral strains such as gene therapy vectors and oncolytic viruses (OV) to selectively destroy cancer cells is poised make a major impact in the clinic revolutionize therapy. In particular, several studies have shown that OV safe well tolerated humans can infect broad range cancers. Yet clinical has highly variable response rates. heterogeneous nature tumors widely accepted be obstacle for therapeutics highlights need strategies improve replication efficacy....
Substances of misuse are becoming increasingly difficult to analyze as unique methods smuggling adopted and due the rapid emergence new psychoactive substances, increasing pool compounds characterize identify. Technologies such gas chromatography liquid coupled mass spectrometry (MS) represent gold standard for accurate robust analysis, with on-site ambient- portable-MS systems providing drug screening testing. For many samples containing residual analyte quantities, improve sensitivity...
The first clean, high-yield route is presented to methylidenes RuCl2(L)(PCy3)(CH2) (L = H2IMes or IMes), key vectors for catalysis and deactivation in many olefin metathesis reactions. (H2IMes N,N′-bis(mesityl)imidazolin-2-ylidene; IMes N,N′-bis(mesityl)imidazol-2-ylidene).
Defining cellular processes relies heavily on elucidating the temporal dynamics of proteins. To this end, mass spectrometry (MS) is an extremely valuable tool; different MS-based quantitative proteomics strategies have emerged to map protein over course stimuli. Herein, we disclose our novel strategy with unique analytical characteristics. By passing ethereal diazomethane peptides strong cation exchange resin within a microfluidic device, react contain fixed, permanent positive charges....
Tagging events with the coincident detection of a barium ion would greatly reduce background for neutrino-less double beta decay search in xenon. This paper describes progress towards realizing this goal. It outlines source that can produce large quantities Ba++ gas, shows be extracted to vacuum, and demonstrates mechanism by which efficiently converted Ba+ as required laser identification.
Slight changes in the abundance of certain lipid species brain may drastically alter normal neurodevelopment via membrane stability, cell signalling, and survival. Previous findings have demonstrated that postnatal exposure to di (2-ethylhexyl) phthalate (DEHP) disrupts axonal neural development hippocampus. The goal current study was determine whether DEHP alters profile hippocampus during development. Systemic treatment with 10 mg/kg led elevated levels phosphatidylcholine sphingomyelin...
Trimethylation enhancement using diazomethane (TrEnDi) is a derivatization technique that significantly enhances the signal intensity of glycerophospholipid species in mass spectrometry (MS) and tandem (MS/MS) analyses. Here, we describe novel apparatus able to conduct situ TrEnDi (iTrEnDi) by generating immediately reacting small amounts gaseous diazoalkane with analyte molecules. iTrEnDi allows complete rapid methylation phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic...
The identification and quantitation of plasmalogen glycerophospholipids is challenging due to their isobaric overlap with plasmanyl ether-linked glycerophospholipids, susceptibility acid degradation, typically low abundance in biological samples. Trimethylation enhancement using diazomethane (TrEnDi) can be used significantly enhance the signal through creation quaternary ammonium groups producing fixed positive charges 13C-diazomethane complex lipid extracts. Although TrEnDi requires a...
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the skeletal muscle of euthermic and torpid Ictidomys tridecemlineatus was purified to electrophoretic homogeneity using a novel method involving Blue-agarose Phenyl-agarose chromatography. Kinetic analysis enzymes isolated two conditions suggested existence structurally distinct proteins, with GAPDH V max being 40–60% less for enzyme condition (in both glycolytic gluconeogenic directions) as compared form. Thermal denaturation, in part...
Methylation of phospholipids (PL) leads to increased uniformity in positive electrospray ionization (ESI) efficiencies across the various PL subclasses. This effect is realized approach referred as "trimethylation enhancement using 13C-diazomethane" (13C-TrEnDi), which results methyl esterification all acidic sites and conversion amines quaternary ammonium sites. Collision-induced dissociation (CID) these cationic modified lipids enables class identification by forming distinctive headgroup...
Lipidomics is a well-established field, enabled by modern liquid chromatography mass spectrometry (LCMS) technology, rapidly generating large amounts of data. Lipid extracts derived from biological samples are complex and most spectral features in LCMS lipidomics datasets remain unidentified, colloquially termed “dark matter”. In-depth analyses triacylglycerol, diacylglycerol, cholesterol ester species revealed the expected ammoniated sodiated ions as well 5 additional higher dark matter...
Lentiviral Vectors (LV) are emerging tools for genetic therapies and novel cancer treatments. While effective, LV-based have extremely large costs associated with their manufacturing delivery. LV technology descends from human immunodeficiency virus (HIV), whose lipid envelope has been previously measured shown to a direct impact on its transduction efficiency. We developed rapid, robust, sensitive untargeted lipidomics pipeline analyze biotherapeutic products demonstrate utility HEK 293T...
The investigation of protein quaternary structure, protein-cofactor, and protein—ligand interactions by mass spectrometry is often limited the fragility such under experimental conditions. To develop more gentle conditions perhaps general use, we used as a model for study oxygenase domain murine inducible nitric oxide synthase (iNOS), which homodimeric, binds heme tetrahydrobiopterin H4B cofactors, substrate L-arginine. energetics collisions in q2 lens region spectrometer were manipulated...
Shotgun lipidomics provides sensitive and fast lipid identification without the need for chromatographic separation. Challenges faced by shotgun analysis of glycerophospholipids (GPs) include lack signal uniformity across GP classes inability to determine carbon–carbon double bond (C═C) location within fatty acyl chains an unsaturated species. Two distinct derivatization strategies were employed both enhance ionization GPs, via trimethylation enhancement using 13C-diazomethane (13C-TrEnDi),...
Context: At elevated temperatures, studies have shown that serum albumin undergoes irreversible changes to its secondary structure. Anionic fatty acids and/or anionic surfactants been stabilize human (HSA) against thermal denaturation through bridging hydrophobic domains and cationic amino residues of the protein.
ABSTRACT Seasonal modifications in the structure of cellular membranes occur as an adaptive measure to withstand exposure prolonged environmental change. Little is known about whether such changes independently external cues, photoperiod or temperature, how they may impact central nervous system. We compared membrane properties neurons isolated from retina goldfish (Carassius auratus), organism well adapted extreme change, during summer and winter months. Goldfish were maintained a facility...